Large sample sampling was adopted in this paper for the statistical analysis of leaves of four populations of Vetiveria zizanioides ( introduced, local wild, domesticated wild populations of different years). The re...Large sample sampling was adopted in this paper for the statistical analysis of leaves of four populations of Vetiveria zizanioides ( introduced, local wild, domesticated wild populations of different years). The results showed that four populations varied in different degrees in terms of tiller height, leaf biomass, and leaf biomass ratio. Leaf length, leaf width, and ratio of leaf width to length of different populations also varied, but had similar power-function change regularity, and showed converging leaf biomass growth pattern and leaf growth process.展开更多
This study was conducted to investigate the gene expression in fructose-fed rat skeletal muscle by cDNA chip which could provide support to elucidate the molecular mechanisms underlying insulin resistance. The rats we...This study was conducted to investigate the gene expression in fructose-fed rat skeletal muscle by cDNA chip which could provide support to elucidate the molecular mechanisms underlying insulin resistance. The rats were divided into two groups, one of which was normal control and the other was fed with fructose-rich diet. The mRNA was isolated and purified from the skeletal muscle of two groups. The mRNA from two kinds of tissue was reverse transcribed to cDNA with Cy3-dUTP and Cy5-dUTP separately to prepare hybridization probes. The mixed probes were hybridized to cDNA microarray. The microarray was scanned, analyzed and repeated for two times. Among the total 4 096 tested genes, 140 genes were differently expressed, 62 up-regulated,78 down-regulated, the expression of Ptprd and Gilz and multiple genes of oxidative metabolism is associated in insulin resistance. The differential expression of gene may be related to the pathogenesis of insulin resistance.展开更多
基金Sponsored by National Spark Program(2012GA780024)Program of Marginal Tropical Characteristic Plant Resources Development Center of Guangdong Universities and Colleges(GCZX-B1002)
文摘Large sample sampling was adopted in this paper for the statistical analysis of leaves of four populations of Vetiveria zizanioides ( introduced, local wild, domesticated wild populations of different years). The results showed that four populations varied in different degrees in terms of tiller height, leaf biomass, and leaf biomass ratio. Leaf length, leaf width, and ratio of leaf width to length of different populations also varied, but had similar power-function change regularity, and showed converging leaf biomass growth pattern and leaf growth process.
基金Supported by"125"Major Scientific and Technological Project of Education Department of Guizhou Province[QJHZDZXZ(2012)013]Guiyang Science and Technology Bureau Project[2012204]
文摘This study was conducted to investigate the gene expression in fructose-fed rat skeletal muscle by cDNA chip which could provide support to elucidate the molecular mechanisms underlying insulin resistance. The rats were divided into two groups, one of which was normal control and the other was fed with fructose-rich diet. The mRNA was isolated and purified from the skeletal muscle of two groups. The mRNA from two kinds of tissue was reverse transcribed to cDNA with Cy3-dUTP and Cy5-dUTP separately to prepare hybridization probes. The mixed probes were hybridized to cDNA microarray. The microarray was scanned, analyzed and repeated for two times. Among the total 4 096 tested genes, 140 genes were differently expressed, 62 up-regulated,78 down-regulated, the expression of Ptprd and Gilz and multiple genes of oxidative metabolism is associated in insulin resistance. The differential expression of gene may be related to the pathogenesis of insulin resistance.