A revision of the genus Chrysotus Meigen from Sichuan,China is presented.Sixteen species are recorded.One species is described as new to science:Chrysotus shuensis Liu&Yang sp.nov.
Background and Aims:Drug-resistant DNA mutations of the hepatitis B virus(HBV)affect treatment response in chronic hepatitis B patients.We have established a new,sensitive,specific,accurate and convenient real-time PC...Background and Aims:Drug-resistant DNA mutations of the hepatitis B virus(HBV)affect treatment response in chronic hepatitis B patients.We have established a new,sensitive,specific,accurate and convenient real-time PCR method to detect HBV mutations quantitatively.Methods:Blood samples were collected from patients showing viral breakthrough,primary nonresponse,or poor response during treatment,and mutations were detected via direct sequencing to assess our method.A plasmid containing the M204V mutation was synthesized and standard curves plotted.Results:The determination coefficient for linear correlation between Ct and log plasmid copy numbers was 0.996,where Ct value was−3.723log(DNA concentration)+48.647.Coefficients of variation indicated good reproducibility.Correctness was within tolerable bias.Limit of detection was 103 copies/mL.Specificity,accuracy,positive predictive value and negative predictive value were 92.86%,100%,96.88%,100%and 94.74%,respectively.Conclusions:These results show that our method can be used to detect HBV M204V mutations with the advantages of sensitivity,specificity and efficiency,providing a new choice for monitoring drug resistance.展开更多
基金supported by the Guangxi Natural Science Foundation (2018GXNSFAA294016)。
文摘A revision of the genus Chrysotus Meigen from Sichuan,China is presented.Sixteen species are recorded.One species is described as new to science:Chrysotus shuensis Liu&Yang sp.nov.
基金This work was supported by funds from the Beijing Municipal Science&Technology Commission(No.Z171100001017037)the Chinese National Science and Technology Major Project(No.2017ZX10105015002).
文摘Background and Aims:Drug-resistant DNA mutations of the hepatitis B virus(HBV)affect treatment response in chronic hepatitis B patients.We have established a new,sensitive,specific,accurate and convenient real-time PCR method to detect HBV mutations quantitatively.Methods:Blood samples were collected from patients showing viral breakthrough,primary nonresponse,or poor response during treatment,and mutations were detected via direct sequencing to assess our method.A plasmid containing the M204V mutation was synthesized and standard curves plotted.Results:The determination coefficient for linear correlation between Ct and log plasmid copy numbers was 0.996,where Ct value was−3.723log(DNA concentration)+48.647.Coefficients of variation indicated good reproducibility.Correctness was within tolerable bias.Limit of detection was 103 copies/mL.Specificity,accuracy,positive predictive value and negative predictive value were 92.86%,100%,96.88%,100%and 94.74%,respectively.Conclusions:These results show that our method can be used to detect HBV M204V mutations with the advantages of sensitivity,specificity and efficiency,providing a new choice for monitoring drug resistance.
