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A Case Study on the Effectiveness of Pre-Class Activities in Higher Vocational English Classes: Taking Pingliang Vocational and Technical College as an Example
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作者 Liuna Yang 《Journal of Contemporary Educational Research》 2024年第8期233-237,共5页
Based on Bloom’s“effective teaching theory,”this paper proposes pre-class activities to improve students’enthusiasm and autonomy in English learning.These include sentence construction,situational dubbing,famous q... Based on Bloom’s“effective teaching theory,”this paper proposes pre-class activities to improve students’enthusiasm and autonomy in English learning.These include sentence construction,situational dubbing,famous quotes,English songs,English games,Chinese stories,regional cultures,and theme debates,which combine life,interest,and inspiration as the main characteristics.The activities can enhance the sense of participation and achievement of all students to achieve the purpose of effective teaching. 展开更多
关键词 Higher vocational college English class Pre-class activity EFFECTIVENESS
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Connotation and Construction of "1+X" Certificate System in Higher Vocational Colleges : Taking Hetian Vocational and Technical College as an Example 被引量:1
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作者 Tuerhong AIZEMAITI Huiqin LIU +2 位作者 Hao LIU Mingming WANG Lanke HUANG 《Asian Agricultural Research》 2023年第9期56-59,共4页
This paper emphatically sums up the problems existing in the implementation of"1+X"certificate system by taking horticulture major in higher vocational colleges as an example.From the point of view of the im... This paper emphatically sums up the problems existing in the implementation of"1+X"certificate system by taking horticulture major in higher vocational colleges as an example.From the point of view of the improvement of"1+X"certificate system,the reform of talent training program,the improvement of teachers ability and summing up experience,this paper puts forward the updated working mode and ideas to provide strategies for accelerating the development of vocational education in southern Xinjiang. 展开更多
关键词 "1+X"certificate system Pilot situation Problems Strategies
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Innovation and Practice of Internal Quality Assurance System in Higher Education Institutions -- Taking Jingzhou Vocational and Technical College as an Example 被引量:1
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作者 Zhang Qing Fang Fengbo +2 位作者 Tang Qinlin Yao Zhiheng Chen Chen 《教育技术与创新》 2023年第3期1-10,共10页
Continuously improving the internal quality assurance system and promoting the innovative development of higher vocational education is a challenge that every higher vocational institution must solve on the road of di... Continuously improving the internal quality assurance system and promoting the innovative development of higher vocational education is a challenge that every higher vocational institution must solve on the road of diagnosis and improvement.To address the current dilemma of building internal quality assurance systems in higher education institutions,this study utilizes a case study approach.This study takes Jingzhou Vocational and Technical College as the research object for research.Conclusion:The excellent diagnosis and reform model and the effectiveness of diagnosis and reform in this school can provide new strategic ideas for the practice of diagnosis and reform for the high-quality development of higher education in Hubei. 展开更多
关键词 diagnosis and improvement internal quality assurance system higher education research
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Campus Construction Featuring Plant Landscapes: A Case Study of Guang'an Vocational & Technical College
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作者 YAO Dequan 《Journal of Landscape Research》 2019年第2期4-7,共4页
SStarting from the analysis of the significance of characteristic plant landscapes in the campus, this paper took Guang'an Vocational & Technical College for example, combined with the greening practice of the... SStarting from the analysis of the significance of characteristic plant landscapes in the campus, this paper took Guang'an Vocational & Technical College for example, combined with the greening practice of the college, designed the planning layout of "one axis, two phases, three rings, and five areas" of the college, and put forward suggestions for campus construction featuring plant landscapes. 展开更多
关键词 PLANT landscapes Characteristics CAMPUS
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Environmental Cultural Landscape Characteristics of Agricultural Vocational Colleges: A Case Study of Bioengineering Branch of Yangling Vocation and Technical College and Doucun Farm
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作者 CHEN Qi WANG Zhousuo ZHU Zhanbin 《Journal of Landscape Research》 2015年第4期25-28,共4页
Based on the overall planning of environmental cultural construction of Yangling Vocational & Technical College and the college–enterprise co-construction of Doucun Farm, this paper combined environmental cultura... Based on the overall planning of environmental cultural construction of Yangling Vocational & Technical College and the college–enterprise co-construction of Doucun Farm, this paper combined environmental cultural reformation inside and outside the college and reform of off-campus teaching experiment farm in view of outstanding agricultural characteristics of the bioengineering branch, with environmental health preservation as the fundamental goal and "health-preserving agriculture" as the link. In addition, four major characteristic landscape frameworks of "point, line, surface, and body" were established, and the effective construction approaches of health-preserving environmental cultural landscapes in vocational colleges were explored. 展开更多
关键词 Bioengineering Branch Health-preserving agriculture Environmental cultural construction
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An Empirical Study on the Interlanguage Errors of Students English Writing in Vocational and Technical Colleges
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作者 He Yihui 《International Journal of Technology Management》 2013年第12期9-11,共3页
This article develops an empirical an investigation on the errors in compositions written by students who are studying in Luzhou Vocational and Technical College (LVTC) and Luzhou Medical College (LMC) and contras... This article develops an empirical an investigation on the errors in compositions written by students who are studying in Luzhou Vocational and Technical College (LVTC) and Luzhou Medical College (LMC) and contrasts them in order to trace the source of the errors. It finally turns out to be that there exists strong correlation between students' academic quality and the number of errors of various types. Compared with ordinary university students, vocational and technical college (VTC) students' interlanguage (IL) errors seem to be more rudimental, and more negative-transferring, most of which are influenced by their mother tongue (MT)-Chinese. VTC students' general error in writing is misspelling, while university students ' major error lies in collocation. In this thesis, some practical enlightenment about learning and teaching English writing are also proposed. 展开更多
关键词 empirical study interlanguage errors analysis English writing VTC students
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The Relationship between Self-Esteem and Social Phobia among College Students:The Mediating Effect of Fear of Negative Evaluation and the Moderating Effect of Perfectionism
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作者 Shuai Lv Zhaojun Chen +3 位作者 Jian Mao Hui Wang Peibo Wu Yujuan Hai 《International Journal of Mental Health Promotion》 2024年第6期491-498,共8页
Objectives:To explore the relationship between college students’self-esteem(SE)and their social phobia(SP),as well as the mediating role of fear of negative evaluation(FNE)and the moderating effect of perfectionism.M... Objectives:To explore the relationship between college students’self-esteem(SE)and their social phobia(SP),as well as the mediating role of fear of negative evaluation(FNE)and the moderating effect of perfectionism.Methods:A convenience sampling survey was carried out for 1020 college students from Shandong Province of China,utilizing measures of college students’self-esteem,fear of negative evaluation,perfectionism,and social phobia.Data analysis was performed using the SPSS PROCESS macro.Results:(1)college students’self-esteem significantly and negatively predicts their social phobia(β=−0.31,t=−10.10,p<0.001);(2)fear of negative evaluation partially mediates the relation between self-esteem and social phobia among college students,with the mediating effect accounting for 48.97%of the total effect(TE);(3)the mediating role of fear of negative evaluation is moderated by perfectionism(β=0.18,t=7.75,p<0.001),where higher levels of perfectionism strengthen the mediating effect of fear of negative evaluation.Conclusions:Perfectionism moderates the mediating effect that fear of negative evaluation plays,establishing a moderated mediating model. 展开更多
关键词 SELF-ESTEEM fear of negative evaluation PERFECTIONISM social phobia
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Study on How to Improve Tourism English Major Students’English Communicative Ability in Higher Vocational College
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作者 SHEN Hua-ying 《Journal of Literature and Art Studies》 2024年第8期726-729,共4页
Tourism English Major students’English communicative ability is very important for students’to promote professional skills.It is necessary for students to improve the commnunicative ability and It is one of the teac... Tourism English Major students’English communicative ability is very important for students’to promote professional skills.It is necessary for students to improve the commnunicative ability and It is one of the teachers’main tasks to cultivate students’communicative ability. 展开更多
关键词 communicative ability professional skills CULTIVATE
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Study of The Technical Index of Online Learning Behavior Analysis of Nursing Majors on The Superstar Platform Based on The Kirkpatrick Evaluation Model
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作者 Yi Zhang Xiaohua Zhao Jie Li 《Journal of Clinical and Nursing Research》 2024年第4期284-291,共8页
Objective:To analyze the technical indexes of students’online learning behavior analysis based on Kirkman’s evaluation model,sort out the basic indexes of online learning behavior,and extract scientific and efficien... Objective:To analyze the technical indexes of students’online learning behavior analysis based on Kirkman’s evaluation model,sort out the basic indexes of online learning behavior,and extract scientific and efficient evaluation indexes of online learning effect through statistical analysis.Methods:The online learning behavior data of Physiology of nursing students from 2021-2023 and the first semester of 22 nursing classes(3 and 4)were collected and analyzed.The preset learning behavior indexes were analyzed by multi-dimensional analysis and a correlation analysis was conducted between the indexes and the final examination scores to screen for the dominant important indexes for online learning effect evaluation.Results:The study found that the demand for online learning of nursing students from 2021-2023 increased and the effect was statistically significant.Compared with the stage assessment results,the online learning effect was statistically significant.Conclusion:The main indicators for evaluating and classifying online learning behaviors were summarized.These two indicators can help teachers predict which part of students need learning intervention,optimize the teaching process,and help students improve their learning behavior and academic performance. 展开更多
关键词 Kirkpatrick assessment model Superstar platform Online learning behavior Analyzing technical indicators Research
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A Study on the Work Process-Based Practical Training Model for Basic Nursing Skills in Vocational Colleges
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作者 Dan Li Huan Wei 《Journal of Clinical and Nursing Research》 2024年第9期128-132,共5页
Objective:To explore and analyze the work process-based practical training teaching model for basic nursing skills in vocational colleges and its implementation effects.Methods:A total of 82 nursing students from our ... Objective:To explore and analyze the work process-based practical training teaching model for basic nursing skills in vocational colleges and its implementation effects.Methods:A total of 82 nursing students from our school were selected for the study,which was conducted from April 2023 to April 2024.Using a random number table method,the students were divided into an observation group and a control group,each with 41 students.The control group received conventional practical training teaching,while the observation group followed the work process-based practical training model for basic nursing skills.The assessment scores and teaching satisfaction of the two groups were compared.Results:The comparison of assessment scores showed that the observation group performed significantly better than the control group(P<0.05).The comparison of teaching satisfaction also indicated that the observation group had significantly higher satisfaction than the control group(P<0.05).Conclusion:The work process-based practical training teaching model for basic nursing skills in vocational colleges can improve students’assessment scores and enhance teaching satisfaction,demonstrating its value for wider application. 展开更多
关键词 Work processes Vocational colleges Basic nursing skills Practical training teaching model
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Current Situation and Countermeasures of the Construction of “Double-Qualified” Teachers in Higher Vocational Colleges
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作者 Hong Ye Yuan Zhong Yanping Chen 《Journal of Contemporary Educational Research》 2024年第8期215-219,共5页
The construction of“double-qualified”teachers is the basic premise for the high-quality development of higher vocational education,and it is also the key force for higher vocational colleges to cultivate high-qualit... The construction of“double-qualified”teachers is the basic premise for the high-quality development of higher vocational education,and it is also the key force for higher vocational colleges to cultivate high-quality skilled talents.This paper probes into the positioning of the construction of“double-qualified”teachers in higher vocational colleges,deeply analyzes the current situation of the construction of“double-qualified”teachers in higher vocational colleges,and provides the countermeasures of the construction of“double-qualified”teachers. 展开更多
关键词 Higher vocational colleges “Double-qualified”teachers Current situation COUNTERMEASURE
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Investigation of the Current Situation of Innovation Ability Education of Vocational College Students Under the Guidance of Ideological and Political Education
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作者 Xiaolin Fan Nurjulidezi Baheti Yonghong Gu 《Journal of Contemporary Educational Research》 2024年第3期73-77,共5页
Innovation ability is the ability to continuously provide new ideas,theories,methods,and inventions with economic,social,and ecological values in the fields of technology and various practical activities.The ideologic... Innovation ability is the ability to continuously provide new ideas,theories,methods,and inventions with economic,social,and ecological values in the fields of technology and various practical activities.The ideological and political education of vocational school students should be student-oriented and close to reality and life.It is necessary to improve the pertinence,effectiveness,attractiveness,and infectiousness of ideological and political education,and cultivate socialist builders and successors with all-round development of morality,intelligence,physique,and beauty.In this paper,we investigate and understand the practice of innovation ability education in colleges and universities at home and abroad,especially the current situation of professional practice teaching in Xinjiang Vocational Colleges.The construction of a professional practice base in vocational colleges is systematically studied. 展开更多
关键词 Ideological and political education Innovation ability education Vocational college students
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Discussion on Talent Training Mode of Engineering Cost Major in Higher Vocational Colleges
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作者 Zhe Yang Yuan Zhong Hong Ye 《Journal of Contemporary Educational Research》 2024年第9期181-185,共5页
With the rapid development of higher vocational education,the number of schools and enrollment has increased rapidly,and each specialty has been continuously refined.This has led to new challenges in the talent traini... With the rapid development of higher vocational education,the number of schools and enrollment has increased rapidly,and each specialty has been continuously refined.This has led to new challenges in the talent training of new specialties in higher vocational colleges in recent years.Based on the post ability standard of cost staff and the skill requirements of related posts,according to the characteristics of this major and guided by the idea of systematization of the work process,this paper discusses the problems existing in the talent training mode of higher vocational engineering cost major and provides some suggestions for the talent training and construction of higher vocational engineering cost major.It aims to promote continuous teaching reform in the future and the development of engineering cost majors in higher vocational colleges. 展开更多
关键词 Higher vocational college Engineering cost major Talent training mode
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Discussions on the Construction of“Double-Qualified”Teachers in Higher Vocational Colleges
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作者 Hong Ye Yuan Zhong Yanping Chen 《Journal of Contemporary Educational Research》 2024年第7期234-239,共6页
Creating a“double-qualified”teacher team is an important content in the construction of a higher vocational college teacher team.This paper probes into the significance of the construction of“double-qualified”teac... Creating a“double-qualified”teacher team is an important content in the construction of a higher vocational college teacher team.This paper probes into the significance of the construction of“double-qualified”teachers in higher vocational colleges,and deeply analyzes the problems existing in the construction of“double-qualified”teachers’training base,the institutionalization of“double-qualified”teacher rotation training,the regularization of cooperation with enterprises,and the rationalization of incentive mechanism.Therefore,it is of great practical significance to strengthen the theoretical research and practical exploration of the construction of“double-qualified”teachers. 展开更多
关键词 “Double-qualified”teachers Team construction Higher vocational colleges
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Effect of comprehensive nursing on the quality of life and swallowing function in individuals diagnosed with ischemic stroke 被引量:1
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作者 Heng-Fen Hu Yu-Fei Sang Yan-Qing Xiao 《World Journal of Clinical Cases》 SCIE 2024年第8期1406-1415,共10页
BACKGROUND Ischemic stroke(IS)is a widely recognized disease characterized by high preva-lence,mortality,morbidity,disability,and recurrence rates.It ranks prominently in terms of mortality,constituting 60%-80%of stro... BACKGROUND Ischemic stroke(IS)is a widely recognized disease characterized by high preva-lence,mortality,morbidity,disability,and recurrence rates.It ranks prominently in terms of mortality,constituting 60%-80%of stroke cases.AIM To explore the impact of comprehensive nursing care on the quality of life and swallowing function in individuals diagnosed with IS.METHODS This study comprised 172 patients with IS admitted to our hospital between February 2018 to March 2021.The participants were divided into two groups,namely the control group(n=80)receiving routine care and the research group(n=92)receiving comprehensive care.Various assessment scales,including the standard swallowing function assessment scale(SSA),National Institutes of Health Stroke scale(NIHSS),European stroke scale(ESS),self-rating anxiety scale(SAS),self-rating depression scale(SDS),Barthel index(BI),and the motor func-tion assessment scale(MAS),were employed to evaluate the improvement in swallowing function,neurological deficits,clinical outcomes,anxiety,depression,daily living activities,and motor function before and after care.Furthermore,the study compared the occurrence of adverse reactions during the nursing period,life quality before and after the intervention,rehabilitation compliance,and nursing satisfaction between the two groups.RESULTS After the nursing intervention,the research group exhibited significantly improved SSA and NIHSS scores compared to the control group(P<0.05).Moreover,both groups demonstrated significant reductions in SAS and SDS scores(P<0.05),with the research group showing more obvious advantages(P<0.05).Compared to the control group,the research group displayed significantly better ESS,BI,and MAS scores(P<0.05),coupled with a lower incidence of adverse reactions(P<0.05).Additionally,the research group demonstrated markedly higher levels of life quality,rehabilitation compliance,and nursing satisfaction compared to the control group(P<0.05).CONCLUSION Comprehensive nursing effectively improved swallowing function,quality of life,and patient satisfaction,high-lighting its clinical significance. 展开更多
关键词 Comprehensive nursing STROKE Swallowing function Quality of life Patient satisfaction
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Microstructure,interfacial reaction behavior,and mechanical properties of Ti_(3)AlC_(2)reinforced Al6061 composites
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作者 Zhi-bin LIU Jia-bao BAO +1 位作者 Wen-jie HU Hong YAN 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2024年第9期2756-2771,共16页
The interfacial reaction behavior of Al and Ti_(3)AlC_(2)at different pouring temperatures and its effect on the microstructure and mechanical properties of the composites were investigated.The results show that the a... The interfacial reaction behavior of Al and Ti_(3)AlC_(2)at different pouring temperatures and its effect on the microstructure and mechanical properties of the composites were investigated.The results show that the addition of3.0 wt.%Ti_(3)AlC_(2)refines the average grain size ofα(Al)in the composite by 50.1%compared to Al6061 alloy.Morphological analyses indicate that an in-situ Al_(3Ti)transition layer of-180 nm in thickness is generated around the edge of Ti_(3)AlC_(2)at 720℃,forming a well-bonded Al-Al_(3Ti)interface.At this processing temperature,the ultimate tensile strength of A16061-3.0 wt.%Ti_(3)AlC_(2)composite is 199.2 MPa,an improvement of 41.5%over the Al6061 matrix.Mechanism analyses further elucidate that 720℃is favourable for forming the nano-sized transition layer at the Ti_(3)AlC_(2)edges.And,the thermal mismatch strengthening plays a dominant role in this state,with a strengthening contribution of about 74.8%. 展开更多
关键词 Al6061 composites Ti_(3)AlC_(2) MICROSTRUCTURE interfacial reaction tensile mechanical properties
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Long non-coding RNA FPFSC promotes immature porcine Sertoli cell growth through modulating the miR-326/EHMT2 axis
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作者 Dan Chu Bin Chen +4 位作者 Bo Weng Saina Yan Yanfei Yin Xiangwei Tang Maoliang Ran 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2024年第11期3830-3842,共13页
Sertoli cells are indispensable for guaranteeing normal spermatogenesis and male fertility.Although a huge number of long non-coding RNAs(lncRNAs)are identified from developing porcine testicular tissues and have been... Sertoli cells are indispensable for guaranteeing normal spermatogenesis and male fertility.Although a huge number of long non-coding RNAs(lncRNAs)are identified from developing porcine testicular tissues and have been predicted with crucial regulatory roles in spermatogenesis,their functions and regulatory mechanisms are still in infancy.Herein,we mainly explored the regulatory and functional roles of lncFPFSC in proliferation and apoptosis of immature porcine Sertoli cells.The results demonstrated that lncFPFSC was predominantly located in the cytoplasm of immature porcine Sertoli cells.lncFPFSC overexpression promoted cell cycle progression and cell proliferation,as well as inhibited cell apoptosis,whereas siRNA-induced lncFPFSC knockdown resulted in the opposite effects.Mechanistically,lncFPFSC acted as a sponge for miR-326.Overexpression of miR-326 inhibited cell proliferation and induced cell apoptosis,which further abolished the effects of lncFPFSC overexpression.The euchromatic histone-lysine N-methyltransferase 2(EHMT2)gene was directly targeted by miR-326,and its mRNA and protein expressions were both negatively regulated by miR-326 in immature porcine Sertoli cells.Then,siRNA-induced EHMT2 knockdown resulted a similar effect of miR-326 inhibition.Collectively,lncFPFSC promoted proliferation and inhibited apoptosis in immature porcine Sertoli cells through modulating the miR-326/EHMT2 axis.This study expanded our understanding of non-coding RNAs in participating porcine spermatogenesis through deciding the fate of Sertoli cells,and the competing endogenous RNA(ceRNA)network,and provided new molecular markers to treat Sertoli cell disorder inducing male infertility. 展开更多
关键词 porcine Sertoli cells PROLIFERATION apoptosis lncRNA FPFSC miR-326 EHMT2 gene
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Examining the Use of Scott’s Formula and Link Expiration Time Metric for Vehicular Clustering
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作者 Fady Samann Shavan Askar 《Computer Modeling in Engineering & Sciences》 SCIE EI 2024年第3期2421-2444,共24页
Implementing machine learning algorithms in the non-conducive environment of the vehicular network requires some adaptations due to the high computational complexity of these algorithms.K-clustering algorithms are sim... Implementing machine learning algorithms in the non-conducive environment of the vehicular network requires some adaptations due to the high computational complexity of these algorithms.K-clustering algorithms are simplistic,with fast performance and relative accuracy.However,their implementation depends on the initial selection of clusters number(K),the initial clusters’centers,and the clustering metric.This paper investigated using Scott’s histogram formula to estimate the K number and the Link Expiration Time(LET)as a clustering metric.Realistic traffic flows were considered for three maps,namely Highway,Traffic Light junction,and Roundabout junction,to study the effect of road layout on estimating the K number.A fast version of the PAM algorithm was used for clustering with a modification to reduce time complexity.The Affinity propagation algorithm sets the baseline for the estimated K number,and the Medoid Silhouette method is used to quantify the clustering.OMNET++,Veins,and SUMO were used to simulate the traffic,while the related algorithms were implemented in Python.The Scott’s formula estimation of the K number only matched the baseline when the road layout was simple.Moreover,the clustering algorithm required one iteration on average to converge when used with LET. 展开更多
关键词 CLUSTERING vehicular network Scott’s formula FastPAM
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Experimental and numerical study of effecting core configurations on the static and dynamic behavior of honeycomb plate with aluminum material
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作者 Nehad Abid Allah Hamza Fatima Mohammed K.AL-Fatlwe Muna Ali Talib 《Defence Technology(防务技术)》 SCIE EI CAS CSCD 2024年第9期177-192,共16页
The sandwich panel incorporated a honeycomb core,a widely utilized composite structure recognized as a fundamental classification of composite materials.Comprised a core resembling a honeycomb,possessing thickness and... The sandwich panel incorporated a honeycomb core,a widely utilized composite structure recognized as a fundamental classification of composite materials.Comprised a core resembling a honeycomb,possessing thickness and softness,and is flank by rigid face sheets that sandwich various shapes and materials.This paper presents an examination of the static and dynamic analysis of lightweight plates made of aluminum honeycomb sandwich composites.Honeycomb sandwich plate samples are 300 mm long,and 300 mm wide,the heights of the core have been varied at four values ranging from 10 to 25 mm.The honeycomb core is manufactured from Aluminum material by using a novel technique namely resistance spot welding(RSW)instead of using adhesive material,which is often used when an industrial flaw is detected.Numerical optimization based on response surface methodology(RSM)and design of experiment software(DOE)was used to verify the current work.A theoretical examination of the crashworthiness behavior(maximum bending load,maximum deflection)and vibration attributes(natural frequency,damping ratio,transient temporal response)of honeycomb sandwich panels with different design parameters was also carried out.In addition,the finite element method-based ANSYS software was used to confirm the theoretical conclusions.The findings of the present work showed that the relationship between the natural frequency,core height,and cell size is direct.In contrast,the relationship between the natural frequency and the thickness of the cell wall is inverse.Conversely,the damping ratio is inversely proportional to the core height and cell size but directly proportional to the thickness of the cell wall.The study indicates that altering the core height within 10-25 mm leads to a significant increase of 82%in the natural frequency and a notable decrease of 49%in the damping ratio.These findings are based on a specific cell size value of 0.01 m and a cell wall thickness of 0.001 m.Also,the results indicate that for a given set of cell wall thickness and size values,an increase in core height from(0.01-0.025)m,leads to a reduction of the percentage of maximum response approX imately 76%.Conversely,the increasing thickness of the wall of cell wall,ranging 0.3-0.7 mm with a constant core height equal to 0.015 m,resulted in a de crease of maximum transient response by 7.8%. 展开更多
关键词 HONEYCOMB Load Transient response Free vibration Natural frequency
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Genome wide association analysis identifies candidate genes for fruit quality and yield in Actinidia eriantha
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作者 Yingzhen Wang Ying Wu +7 位作者 Xinlei Wang Wangmei Ren Qinyao Chen Sijia Zhang Feng Zhang Yunzhi Lin Junyang Yue Yongsheng Liu 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2024年第6期1929-1939,共11页
Quality and yield are the primary concerns in kiwifruit breeding,but research on the genetic mechanisms of fruit size,shape,and ascorbic acid(ASA)content is currently very limited,which restricts the development of ki... Quality and yield are the primary concerns in kiwifruit breeding,but research on the genetic mechanisms of fruit size,shape,and ascorbic acid(ASA)content is currently very limited,which restricts the development of kiwifruit molecular breeding.In this study,we obtained a total of 8.88 million highly reliable single nucleotide polymorphism(SNP)markers from 140 individuals from the natural hybrid offspring of Actinidia eriantha cv.‘White’using whole genome resequencing technology.A genome-wide association study was conducted on eight key agronomic traits,including single fruit weight,fruit shape,ASA content,and the number of inflorescences per branch.A total of 59 genetic loci containing potential functional genes were located,and candidate genes related to single fruit weight,fruit length,ASA content,number of inflorescences per branch and other traits were identified within the candidate interval,such as AeWUSCHEL,AeCDK1(cell cycle dependent kinase),AeAO1(ascorbic oxidase)and AeCO1(CONSTANS-like 4).After constructing an RNAi vector for AeAO1 and injecting it into the fruit of cv.‘Midao 31’to interfere with the expression of the AeAO1 gene,the results showed that the activity of ascorbic oxidase in the fruit of‘Midao 31’significantly decreased,while the content of ASA significantly increased.This study provides valuable insights into the genetic basis of variation in A.eriantha fruit traits,which may benefit molecular marker-assisted breeding efforts. 展开更多
关键词 Actinidia eriantha GWAS SNP QTLS fruit quality
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