Diagnosis and treatment of refractory infectious diseases using nanopore sequencing technology:Three case reports

BACKGROUND Infectious diseases are still one of the greatest threats to human health,and the etiology of 20%of cases of clinical fever is unknown;therefore,rapid identification of pathogens is highly important.Traditional culture methods are only able to detect a limited number of pathogens and are time-consuming;serologic detection has window periods,false-positive and false-negative problems;and nucleic acid molecular detection methods can detect several known pathogens only once.Three-generation nanopore sequencing technology provides new options for identifying pathogens.CASE SUMMARY Case 1:The patient was admitted to the hospital with abdominal pain for three days and cessation of defecation for five days,accompanied by cough and sputum.Nanopore sequencing of the drainage fluid revealed the presence of orallike bacteria,leading to a clinical diagnosis of bronchopleural fistula.Cefoperazone sodium sulbactam treatment was effective.Case 2:The patient was admitted to the hospital with fever and headache,and CT revealed lung inflammation.Antibiotic treatment for Streptococcus pneumoniae,identified through nanopore sequencing of cerebrospinal fluid,was effective.Case 3:The patient was admitted to our hospital with intermittent fever and an enlarged neck mass that had persisted for more than six months.Despite antibacterial treatment,her symptoms worsened.The nanopore sequencing results indicate that voriconazole treatment is effective for Aspergillus brookii.The patient was diagnosed with mixed cell type classical Hodgkin's lymphoma with infection.CONCLUSION Three-generation nanopore sequencing technology allows for rapid and accurate detection of pathogens in human infectious diseases.

Curriculum Reform Practices in “Cosmetic Product Formulation Design and Preparation Technology” Under the Perspective of Integration of Industry, Education, and Research

With the upgrading of industries,the cosmetics industry has posed new requirements for technical talents.As a professional core course in cosmetic technology,“Cosmetic Product Formulation Design and Preparation Technology”serves as the foundation for cultivating students’abilities in cosmetic development and preparation.To foster high-quality skilled talents capable of adapting to the rapid growth of color cosmetics and to better promote the deep integration of scientific and technological industries with curriculum teaching,the teacher team embarked on active explorations and practical teaching research for curriculum teaching reform from four dimensions:strengthening top-level design,enriching teaching content,optimizing teaching design,and reforming assessment methods.These efforts have enhanced students’comprehensive vocational qualities and innovative consciousness,contributing to the teaching reform in higher vocational colleges under the integration of industry,education,and research.

A frequency servo SoC with output power stabilization loop technology for miniaturized atomic clocks

A frequency servo system-on-chip(FS-SoC)featuring output power stabilization technology is introduced in this study for high-precision and miniaturized cesium(Cs)atomic clocks.The proposed power stabilization loop(PSL)technique,incorporating an off-chip power detector(PD),ensures that the output power of the FS-SoC remains stable,mitigating the impact of power fluctuations on the atomic clock's stability.Additionally,a one-pulse-per-second(1PPS)is employed to syn-chronize the clock with GPS.Fabricated using 65 nm CMOS technology,the measured phase noise of the FS-SoC stands at-69.5 dBc/Hz@100 Hz offset and-83.9 dBc/Hz@1 kHz offset,accompanied by a power dissipation of 19.7 mW.The Cs atomic clock employing the proposed FS-SoC and PSL obtains an Allan deviation of 1.7×10^(-11) with 1-s averaging time.

Development and technology status of energy storage in depleted gas reservoirs

Utilizing energy storage in depleted oil and gas reservoirs can improve productivity while reducing power costs and is one of the best ways to achieve synergistic development of"Carbon Peak–Carbon Neutral"and"Underground Resource Utiliza-tion".Starting from the development of Compressed Air Energy Storage(CAES)technology,the site selection of CAES in depleted gas and oil reservoirs,the evolution mechanism of reservoir dynamic sealing,and the high-flow CAES and injection technology are summarized.It focuses on analyzing the characteristics,key equipment,reservoir construction,application scenarios and cost analysis of CAES projects,and sorting out the technical key points and existing difficulties.The devel-opment trend of CAES technology is proposed,and the future development path is scrutinized to provide reference for the research of CAES projects in depleted oil and gas reservoirs.

Development status and prospect of underground thermal energy storage technology

Underground Thermal Energy Storage(UTES)store unstable and non-continuous energy underground,releasing stable heat energy on demand.This effectively improve energy utilization and optimize energy allocation.As UTES technology advances,accommodating greater depth,higher temperature and multi-energy complementarity,new research challenges emerge.This paper comprehensively provides a systematic summary of the current research status of UTES.It categorized different types of UTES systems,analyzes the applicability of key technologies of UTES,and evaluate their economic and environmental benefits.Moreover,this paper identifies existing issues with UTES,such as injection blockage,wellbore scaling and corrosion,seepage and heat transfer in cracks,etc.It suggests deepening the research on blockage formation mechanism and plugging prevention technology,improving the study of anticorrosive materials and water treatment technology,and enhancing the investigation of reservoir fracture network characterization technology and seepage heat transfer.These recommendations serve as valuable references for promoting the high-quality development of UTES.

Application and prospects of proteomic technology in inflammation:a review

Proteomics is a new technology that has been widely applied in the field of life and health science.It effectively addresses issues related to the impact of dietary structure on organs,tissues,and cells,as well as the changes in proteins in various organs,tissues,and cells under disease conditions.The differential proteins identified through proteomics can serve as disease biomarkers and target proteins affecting health and can be used for disease diagnosis and health regulation.In this paper,the application of proteomics in the field of infl ammation in recent years was summarized,especially in the therapeutic target and mechanism of action,which opens up a new way for more effective prevention,diagnosis,and treatment of inflammation,and provides medical protection for human life and health.

Passive IoT Localization Technology Based on SD-PDOA in NLOS and Multi-Path Environments

Addressing the challenges of passive Radio Frequency Identification(RFID)indoor localization technology in Non-Line-of-Sight(NLoS)and multipath environments,this paper presents an innovative approach by introducing a combined technology integrating an improved Kalman Filter with Space Domain Phase Difference of Arrival(SD-PDOA)and Received Signal Strength Indicator(RSSI).This methodology utilizes the distinct channel characteristics in multipath and NLoS contexts to effectively filter out interference and accurately extract localization information,thereby facilitating high precision and stability in passive RFID localization.The efficacy of this approach is demonstrated through detailed simulations and empirical tests conducted on a custom-built experimental platform consisting of passive RFID tags and an R420 reader.The findings are significant:in NLoS conditions,the four-antenna localization system achieved a notable localization accuracy of 0.25 m at a distance of 5 m.In complex multipath environments,this system achieved a localization accuracy of approximately 0.5 m at a distance of 5 m.When compared to conventional passive localization methods,our proposed solution exhibits a substantial improvement in indoor localization accuracy under NLoS and multipath conditions.This research provides a robust and effective technical solution for high-precision passive indoor localization in the Internet of Things(IoT)system,marking a significant advancement in the field.

Prospects for green steelmaking technology with low carbon emissions in China

The steel industry is a major source of CO_(2) emissions,and thus,the mitigation of carbon emissions is the most pressing challenge in this sector.In this paper,international environmental governance in the steel industry is reviewed,and the current state of development of low-carbon technologies is discussed.Additionally,low-carbon pathways for the steel industry at the current time are proposed,emphasizing prevention and treatment strategies.Furthermore,the prospects of low-carbon technologies are explored from the perspective of transitioning the energy structure to a“carbon-electricity-hydrogen”relationship.Overall,steel enterprises should adopt hydrogen-rich metallurgical technologies that are compatible with current needs and process flows in the short term,based on the carbon substitution with hydrogen(prevention)and the CCU(CO_(2) capture and utilization)concepts(treatment).Additionally,the capture and utilization of CO_(2) for steelmaking,which can assist in achieving short-term emission reduction targets but is not a long-term solution,is discussed.In conclusion,in the long term,the carbon metallurgical process should be gradually supplanted by a hydrogen-electric synergistic approach,thus transforming the energy structure of existing steelmaking processes and attaining near-zero carbon emission steelmaking technology.

Theory,technology and application of grouted bolting in soft rock roadways of deep coal mines

The grouted bolt,combining rock bolting with grouting techniques,provides an effective solution for controlling the surrounding rock in deep soft rock and fractured roadways.It has been extensively applied in numerous deep mining areas characterized by soft rock roadways,where it has demonstrated remarkable control results.This article systematically explores the evolution of grouted bolting,covering its theoretical foundations,design methods,materials,construction processes,monitoring measures,and methods for assessing its effectiveness.The overview encompassed several key elements,delving into anchoring theory and grouting reinforcement theory.The new principle of high pretensioned high-pressure splitting grouted bolting collaborative active control is introduced.A fresh method for dynamic information design is also highlighted.The discussion touches on both conventional grouting rock bolts and cable bolts,as well as innovative grouted rock bolts and cables characterized by their high pretension,strength,and sealing hole pressure.An examination of the merits and demerits of standard inorganic and organic grouting materials versus the new inorganic–organic composite materials,including their specific application conditions,was conducted.Additionally,the article presents various methods and instruments to assess the support effect of grouting rock bolts,cable bolts,and grouting reinforcement.Furthermore,it provides a foundation for understanding the factors influencing decisions on grouted bolting timing,the sequence of grouting,the pressure applied,the volume of grout used,and the strategic arrangement of grouted rock bolts and cable bolts.The application of the high pretensioned high-pressure splitting grouted bolting collaborative control technology in a typical kilometer-deep soft rock mine in China—the soft coal seam and soft rock roadway in the Kouzidong coal mine,Huainan coal mining area,was introduced.Finally,the existing problems in grouted bolting control technology for deep soft rock roadways are analyzed,and the future development trend of grouted bolting control technology is anticipated.

Structural insights on anti-biofilm mechanism of heated slightly acidic electrolyzed water technology against multi-resistant Staphylococcus aureus biofilm on food contact surface

Slightly acidic electrolyzed water(SAEW)has proven to be an efficient and novel sanitizer in food and agriculture field.This study assessed the efficacy of SAEW(30 mg/L)at 40℃on the inactivation of foodbome pathogens and detachment of multi-resistant Staphylococcus aureus(MRSA)biofilm.Furthermore.the underlying mechanism of MRS A biofilm under heated SAEW at 40℃treatment on metabolic profiles was investigated.The results showed that the heated SAEW at 40℃significantly effectively against foodbome pathogens of 1.96-7.56(lg(CFU/g))reduction in pork,chicken,spinach,and lettuce.The heated SAEW at 40℃treatment significantly reduced MRS A biofilm cells by 2.41(lg(CFU/cm^(2))).The synergistic effect of SAEW treatment showed intense anti-biofilm activity in decreasing cell density and impairing biofilm cell membranes.Global metabolic response of MRSA biofilms,treated by SAEW at 40℃,revealed the alterations of intracellular metabolites,including amino acids,organic acid,fatty acid,and lipid.Moreover,signaling pathways involved in amino acid metabolism,energy metabolism,nucleotide synthesis,carbohydrate metabolites,and lipid biosynthesis were functionally disrupted by the SAEW at 40℃treatment.As per our knowledge,this is the first research to uncover the potential mechanism of heated SAEW treatment against MRSA biofilm on food contact surface.

English-Chinese Translation of Science and Technology Texts Based on the Differences Between Chinese and Western Modes of Thinking

As a tool for human communication,the use of language is affected by the way of thinking in cross-cultural communication.Due to the differences between Chinese and Western cultures,the way of thinking varies under the influence of such cultural differences.In the process of translation,the influence of the different thinking mode on translation activities cannot be ignored.At present,a lot of studies and researches have focused on the influence of Chinese and Western thinking mode on translation.However,it is still worthwhile to study what specific influence the difference between Chinese and Western thinking mode will exert on the translation process of science texts and what kind of measure should be taken to solve the negative influence brought by the differences when we are translating.This paper aims to discuss the influence of thinking differences between Chinese and Western countries on the process of English-Chinese translation of science and technology from the perspectives of long sentences,passive voice and nominalization,and how the translators should appropriately adjust their thinking pattern and use reasonable skills to eliminate the potential negative interference caused by the difference and achieve a better transformation between English and Chinese.

Research Progress of Atrial Fibrillation Detection Technology

Atrial fibrillation (AF) is the most common chronic arrhythmia in clinical practice, which can cause high disability and mortality with the progress of the disease. Many studies at home and abroad have shown that the incidence of atrial fibrillation gradually increases with age. Clinically, the onset of most AF patients is insidious, which is difficult to capture by routine electrocardiogram, and there is some difficulty in the diagnosis. In order to make the early diagnosis of atrial fibrillation more efficient and accurate, this paper reviews the current status and research progress of detection technology for atrial fibrillation at home and abroad, in order to provide a scientific basis for the early diagnosis of atrial fibrillation.

A new horizon for neuroscience:terahertz biotechnology in brain research

Terahertz biotechnology has been increasingly applied in various biomedical fields and has especially shown great potential for application in brain sciences.In this article,we review the development of terahertz biotechnology and its applications in the field of neuropsychiatry.Available evidence indicates promising prospects for the use of terahertz spectroscopy and terahertz imaging techniques in the diagnosis of amyloid disease,cerebrovascular disease,glioma,psychiatric disease,traumatic brain injury,and myelin deficit.In vitro and animal experiments have also demonstrated the potential therapeutic value of terahertz technology in some neuropsychiatric diseases.Although the precise underlying mechanism of the interactions between terahertz electromagnetic waves and the biosystem is not yet fully understood,the research progress in this field shows great potential for biomedical noninvasive diagnostic and therapeutic applications.However,the biosafety of terahertz radiation requires further exploration regarding its two-sided efficacy in practical applications.This review demonstrates that terahertz biotechnology has the potential to be a promising method in the field of neuropsychiatry based on its unique advantages.

Research of Blockchain Technology in the Traceability of Characteristic Agricultural Products

With the increasingly prominent problem of food safety,the quality traceability of characteristic agricultural products has become a pressing issue.This study focuses on the application of blockchain technology in the traceability of characteristic agricultural products,aiming to explore its potential and practical value in improving the efficiency and transparency of the traceability system of agricultural products.Through the combination of case analysis and model construction,a blockchain-based traceability system for characteristic agricultural products was established.The results showed that the traceability system could effectively record the whole process information of agricultural products from production and processing to sales,and greatly improve the immutability and traceability of data.Lastly,this paper also points out that the use of blockchain technology can improve the market trust in characteristic agricultural products,provide consumers with authentic and reliable product information,and provide new technical means for the quality management of agricultural products.

Application of Digital Technology in Road and Bridge Design

With the development and progress of science and technology,road and bridge design has experienced rapid development,from the initial manual drawing design to the popularity of Computer-Aided Design(CAD),and then to today’s digital software design era.Early designers relied on hand-drawn paper design forms which was time-consuming and error-prone.Digital support for road and bridge design not only saves the design time but the design quality has also achieved a qualitative leap.This paper engages in the application of digital technology in road and bridge design,to provide technical reference for China’s road and bridge engineering design units,to promote the popularity of Civil3D and other advanced design software in the field of engineering design and development,ultimately contributing to the sustainable development of China’s road and bridge engineering.

Research on the Design of Innovative Play Blind Box Based on AR Digital Technology Empowering Traditional Intangible Cultural Heritage

The development of science and technology,especially the rise of augmented reality(AR)technology provides a new way for the inheritance and innovation of traditional intangible cultural heritage.Based on the design and research of the tide play blind box of AR digital technology empowering traditional intangible cultural heritage,this paper discusses the application and feasibility of AR technology in the traditional intangible cultural heritage,analyzes the strategy of implementing AR technology empowering intangible cultural heritage,and puts forward corresponding countermeasures for the challenges that may be faced,aiming at providing new ideas and methods for traditional intangible cultural heritage and modern science and technology.

Research on the Impact of Digital Technology and Servitization on the Performance of Manufacturing Enterprises

This article mainly discusses the impact of digital technology and service-oriented on the performance of manufacturing enterprises.It first elaborates on the background of the trend of service-oriented manufacturing and the significance of its enterprises.Then,based on the impact of digital technology on the performance of manufacturing enterprises,several practical and feasible measures are proposed,mainly focusing on the long-term layout of service-oriented,shortening the low period,enhancing the competitive awareness of state-owned manufacturing enterprises,improving the driving force transformation,achieving simultaneous progress digitally,configuring digital and service-oriented elements,and eliminating the monopoly of digital resources.Followed by lowering the threshold for private enterprise resource acquisition,promoting financial digital development,breaking the financial dilemma of service-oriented manufacturing,continuously improving the performance level of manufacturing enterprises,and promoting the development of manufacturing enterprises.

Research on the Application of Blockchain Technology in Cold Chain Logistics for Agricultural Products

With the continuous advancement of science and technology and the advent of the digital era,blockchain technology is increasingly demonstrating its great potential across various industries due to its unique features of decentralization,transparency,and immutability.In particular,in the cold chain logistics of agricultural products,the application of blockchain technology can not only enhance logistics efficiency but also ensure food safety and boost consumer trust[1].This paper first outlines the advantages of blockchain technology in cold chain logistics for agricultural products,and then clarifies the application pathways of blockchain technology in this field,providing a reference for relevant researchers.

Analysis of Municipal Water Supply and Drainage Pipe Design Technology

The quality and safety of residents’water rely heavily on the design of municipal water supply and drainage pipes.Therefore,this paper aims to enhance the optimization of municipal water supply and drainage pipe design by focusing on design requirements,principles,and key elements.Drawing from relevant design optimization experiences,technology advancements,and optimization measures,the research will analyze and consolidate the essential aspects of municipal water supply and drainage pipe design.The goal is to fundamentally elevate the quality standards of these designs,ensuring they meet the criteria for engineering project excellence.Through this comprehensive approach,we aim to contribute to the improvement and sustainability of water supply and drainage systems,safeguarding the well-being of residents.

Application Strategy of BIM Technology in the Forward Design of Bridges

Bridge engineering is highly specialized and has spatial characteristics,which puts forward higher requirements for design work.The advancement of information technology has provided ample tools to facilitate bridge design work,with building information modeling(BIM)technology being one of them.BIM technology ensures the efficiency and quality of the forward design of bridges,while also reducing construction costs.This article starts with defining the concept of BIM technology,followed by a discussion on its advantages in bridge design and application process,which serves as a reference for other bridge designers.