Comparative Analysis on Chemical Components and Sensory Quality of Aging Flue-Cured Tobacco from Four Main Tobacco Areas of China

Complicated changes are happening during the aging process of flue-cured tobacco （FCT） and understanding of the changing components is of great significance in improving the quality,shortening aging time,and enhancing production and economic efficiency in tobacco industry.The aging samples of FCT from four main producing areas of China,i.e.,Yunnan,Sichuan,Hubei,and Dongbei,were used to study the changing rule of the components such as alkali,acid,and carbohydrate as well as the aroma and their relationship with sensory quality;and based on the correlation among the components and the sensory quality index,multivariable models were established to predict the aging quality.The results showed that the sensory quality of FCT increased steadily during the aging time,and at the same time,the acidic components （total organic acids,volatile acids） increased gradually,while the alkaline substances （nicotine,volatile alkali）,carbohydrate （total sugars,reducing sugar） and pH values showed a downward trend.Correlation analysis found that the sensory quality and pH values were negatively correlated （P0.05）,while the sensory quality with total organic acids and aroma were positively correlated.The optimal model for predicting the quality of FCT was y=56.75-0.63x12＋50.09x2-13.27x22,（y：sensory quality;x1：pH;x2：total organic acids）.The average predicating error was 1.04% with maximum predicating error of 2.89% and the accuracy rate of above 97%.

Canonical Correlation between the Leaf Quality Indicators of "Moderate Aroma" Flue-cured Tobacco

In order to find out the correlation between tobacco quality evaluation indicators in China's traditional " moderate aroma" tobaccoproducing areas and simplify the tobacco quality evaluation indicators,we evaluate the appearance quality and smoking quality of 143 flue-cured tobacco leaf samples in China's " moderate aroma" tobacco-producing areas,test the physical traits and chemical component,and analyze the canonical correlation between four quality evaluation indicators. The results show that there is significant or extremely significant canonical correlation between four evaluation indicators( tobacco smoking quality,chemical component,appearance quality and physical trait quality); the cumulative variance contribution rate of evaluation indicators is in the order of chemical component( 69. 17%) > appearance quality( 68. 76%) > physical traits( 64. 13%); appearance quality is most closely related to physical traits( 93. 84%). The individual indicators for tobacco quality evaluation make different contribution to the correlation between quality evaluation indicators. The chemical component evaluation indicators mainly include total sugar and ratio of total sugar to betaine; sensory taste indicators mainly include aroma volume,smoke concentration,irritation and softness degree; physical trait evaluation indicators mainly include leaf weight,leaf length and leaf density; appearance quality indicators mainly include leaf organizational structure,color,maturity and identity. Studies have shown that in the large-scale ecoregion,using canonical correlation analysis to simplify tobacco quality evaluation indicators is feasible.

Application of Tobacco Leaves from Shandong in " Taishan" Brand

In view of the fact that the proportion of tobacco leaves from Shandong in the stocks of a company is large,based on the premise of not affecting the quality of cigarettes,through the scientific evaluation,selection and matching test,the proportion of tobacco leaves from Shandong in the formula of " Taishan" cigarette A has been increased,and the sensory quality of " Taishan" cigarette A before and after the test is basically stable.Moreover,the stocks of tobacco leaves from Shandong can be decreased in time.

Study on Extraction of Effective Aroma Components from Formula Tobacco by the Supercritical CO2 Extraction Method and Its Aroma Composition

[Objectives]This study was conducted to improve the sensory quality and industrial availability of tobacco extracts.[Methods]The L9(34)design was adopted to carried out an extraction experiment,in which formula tobacco was extracted using supercritical CO2,and the extract was concentrated by vacuum distillation.Through sensory evaluation and chemical analysis,the function determination and chemical composition analysis of the tobacco extracts were carried out,and the optimal supercritical fluid extraction process was finally determined.[Results]The obtained optimal supercritical fluid extraction conditions were as follows:extraction temperature 55℃,extraction pressure 25 MPa,CO2 flow rate of 20 L/h,and entrainer of 95%ethanol.The tobacco extract obtained under the optimal conditions endowed the cigarettes with full and delicate aroma,less irritation and clean aftertaste and made the flavor of the cigarettes overall coordinated and softer,so the sensory quality was significantly improved.[Conclusions]The tobacco extract obtained by the supercritical CO2 extraction method from formula tobacco can effectively improve the quality of cigarettes.

A possible mechanism of mineral responses to elevated atmospheric CO_2 in rice grains

Increasing attentions have been paid to mineral concentration decrease in milled rice grains caused by CO2 enrichment, but the mechanisms still remain unclear. Therefore, mineral （Ca, Mg, Fe, Zn and Mn） translocation in plant-soil system with a FACE （Free-air CO2 enrichment） experiment were investigated in Eastern China after 4-yr operation. Results mainly showed that： （1） elevated CO2 significantly increased the biomass of stem and panicle by 21.9 and 24.0%, respectively, but did not affect the leaf biomass. （2） Elevated CO2 significantly increased the contents of Ca, Mg, Fe, Zn, and Mn in panicle by 61.2, 28.9, 87.0, 36.7, and 66.0%, respectively, and in stem by 13.2, 21.3, 47.2, 91.8, and 25.2%, respectively, but did not affect them in leaf. （3） Elevated CO2 had positive effects on the weight ratio of mineral/biomass in stem and panicle. Our results suggest that elevated CO2 can favor the translocation of Ca, Mg, Fe, Zn, and Mn from soil to stem and panicle. The CO2-led mineral decline in milled rice grains may mainly attribute to the CO2-1ed unbalanced stimulations on the translocations of minerals and carbohydrates from vegetative parts （e.g., leaf, stem, branch and husk） to the grains.

Research on the Types of Cigarette Products Preferred by Consumers

Cigarette design and improvement according to consumer needs is an important basis for market-oriented management.The preferences of cigarette consumption such as cigarette product types,packaging styles,packaging colors and packaging materials were investigated and the real needs of cigarette consumers were figured out,in order to provide specific work guidance for the development of new cigarette products.

An improved CFD model of gas flow and particle interception in a fiber material

An improved CFD model of gas flow and particle interception in a fiber material which fiber size is Y-shape was developed in this work. The porous medium model was used to build the model of the whole size of fiber filter medium. Mixture model was adopted. The algorithm of particle interception in the whole size of fiber filter medium was derived and UDF(User Defined Function) that described kinds of particle filtering mechanisms in filter fibrous media was added to the Fluent default conservation equation as source term for simulation. The inertial resistance of the filter was taken into consideration, which provided a more precise measurement of the smoke flow and the particle interception in the filter under higher smoke speed conditions. The commercial software, Fluent 6.3, was used to simulate the smoke flow and particle interception in the filter in a single suction. The velocity and pressure profiles of smoke or nicotine particle in the filter, as well as nicotine particle volume fraction profile were well simulated. Finally, the comparisons of nicotine particle filtration efficiency between Fluent simulation results in this work and experimental results, as well as the model prediction in the literature were made to validate the simulation model. The comparisons showed that the particle entrapment model from simulation results was in good agreement with that from the experimental results. In addition, the Fluent simulation results are closer to reality both at the beginning and the end of the smoke process comparing with the model predicted results in the literature.

Degradation Dynamics of Pyrethroids in Cigarette Production Environment

[ Objective] In order to provide a reference for safe and reasonable use of pyrethroids in cigarette production, the degradation behaviors of three kinds of pyrethroids in cigarette production environment were studied. [ Method ] Three kinds of pyrethroids （permethrin, cyfluthrin and c/s-cypermethrin） were adminis- trated to two media （cut tobacco and iron sheet surface） in cigarette production environment at MRLs concentration, and the first sampling was conducted at 24 d post administration. Afterwards, sampling was performed once every 7 d, with a total of six times. The degradation rates of insecticides in different media were cal- culated, and degradation models were established. [ Result ] The contents of three kinds of insecticides in two media gradually declined with the prolongation of time, and degradation rates presented the trend of fast-slow-fast ; the degradation rates of permethrin, cyfluthrin and c/s-cypermethrin in cut tobacco at 36 d post ad- ministration were 97.14%, 88.89% and 95.38%, respectively; the degradation rates on iron sheet surface were 29.95%, 48.99% and 40. 24%, respectively. The half lives of three kinds of insecticides in cut tobacco were 1 -2 d, while those on iron sheet surface were 6 - 10 d. [ Conclusion] The contents of three kinds of insecticides in two media gradually declined with the prolongation of time, and their degradation regularity accorded with first order kinetic equation.

The behaviors of ultra-low-gold-loaded catalysts （Au/ Ce02） for CO oxidation in the presence of water on the catalysts

The catalytic behavior of ultra-low-gold-loaded Au/CeO2 during CO oxidation was studied using HRTEM, TGA, in-situ FTIR, CO--TPD, O2-TPD, and O2-TPO. Herein, water, which facilitates CO oxidation, is present on the catalyst rather than obtained from the reactant gas. The experimental results show that the presence of water on the catalyst enhances the adsorption and activation of 02 to produce Oact on the catalyst surface, resulting in excellent catalytic activity. Different reaction pathways for CO oxidation over Au/CeO2 may be present; however, the dominant pathway is influenced by the species on the catalyst surface.

A selective coumarin-based “turn-on” fluorescent sensor for the detection of cysteine and its applications for bioimaging

A coumarin-based compound（1） was designed and synthesized as a new turn-on fluorescent probe for the detection of cysteine. The probe exhibited higher selectivity towards the target molecule over other thiol and amino acids at pH 7.2 in aqueous media CH_3CN-HEPES（0.02 mol/L, pH 7.2, 1：9, v/v）. The reaction mechanism is attributed to the cysteine-induced S_NAr substitution-rearrangement reaction. Remarkable enhancement of up to 20-fold in fluorescence intensity was achieved in the detection of cysteine. When applied for the fluorescence imaging of cysteine, the compound 1 emitted a green fluorescence in Hi5 cell cytoplasm. The in vivo imaging of Caenorhabditis elegans had further confirmed the cysteine detection by compound 1.

Homogeneous transparent device and its layered realization

Arbitrarily shaped electromagnetic transparent devices with homogeneous, non-negative, anisotropic and generic constitutive parameters are proposed based on linear transformation optics, which provides the flexibility for device design that is applicable for the practical fabrication. To remove the anisotropic property, a layered structure is developed based on effective medium theory. Simulation results show that with sufficient layers, the performance of the layered transparent device is nearly as perfect as an ideal device, and it is able to protect an antenna without sacrificing its performance. The feasibility of designing a transparent device by using natural isotropic materials instead of metamaterials would dramatically reduce the difficulty of fabrication and further promote the practicality of the device.

A rhodamine derivative for Hg^(2+) -selective colorimetric and fluorescent sensing and its application to in vivo imaging

A rhodamine-based sensor has been developed for the detection of mercuric ions. The colorimetric and fluorescence responses, allowing naked-eye detections, are based on Hg^2＋-induced opening of the rhodamine spirocycle. Among all the testes ions, only Hg^2＋generated a significant fluorescence enhancement of up to 300-fold, with a bright yellow–green emission. This sensor was a low toxic compound, and was successfully applied in the in vivo imaging of Hg^2＋in Spill 2 cells and C. elegans. This approach provides a sensitive and accurate method for the estimation of Hg^2＋in environmental, tobacco and biological applications.

Elevated CO_2 facilitates C and N accumulation in a rice paddy ecosystem

Elevated CO2 can stimulate wetland carbon （C） and nitrogen （N） exports through gaseous and dissolved pathways, however, the consequent influences on the C and N pools are still not fully known. Therefore, we set up a free-air CO2 enrichment experiment in a paddy field in Eastern China. After five year fumigation, we studied C and N in the plant-water-soil system. The results showed： （1） elevated CO2 stimulated rice aboveground biomass and N accumulations by 19.1% and 12.5%, respectively. （2） Elevated CO2 significantly increased paddy soil TOC and TN contents by 12.5% and 15.5%, respectively in the 0-15 crn layer, and 22.7% and 26.0% in the 15-30 cm soil layer. （3） Averaged across the rice growing period, elevated CO2 greatly increased TOC and TN contents in the surface water by 7.6% and 11.4%, respectively. （4） The TOC/TN ratio and natural 5ISN value in the surface soil showed a decreasing trend under elevated CO2. The above results indicate that elevated CO2 can benefit C and N accumulation in paddy fields. Given the similarity between the paddies and natural wetlands, our results also suggest a great potential for long-term C and N accumulation in natural wetlands under future climate patterns.

Influencing Factors and Product Toxicity of Anthracene Oxidation by Fungal Laccase

The transformation profiles of polycyclic aromatic hydrocarbons （PAHs） by pure laccases from Trametes versicolor and Pycnoporus sanguineus, and the optimal reaction conditions （acetonitrile concentration, pH, temperature and incubation time） were determined. Anthracene was the most transformable PAH by both laccases, followed by benzo[a]pyrene, and benzo[a]anthracene. Laccase-mediator system （LMS） could not only improve the PAH oxidation but also extend the substrate types compared to laccase alone. 5e/0 or 10~ （v/v） of acetonitrile concentration, pH 4, temperature of 40 ~C, and incubation time of 24 h were most favorable for anthracene oxidation by laccase from T. versicolor or P. sanguineus. The gas chromatography-mass spectrometry analysis indicated that 9,10- anthraquinone was the main product of anthracene transformed by laccase from T. versicolor. Microtox test results showed that both anthracene and its laccase-transformation products were not acute toxic compounds, suggesting that laccase-treatment of anthracene would not increase the acute toxicity of contaminated site.

A Rhodamine-based fluorescent sensor for chromium ions and its application in bioimaging

A rhodamine-based sensor（1） has been developed for the detection of chromium ions.Cr-（3＋）-induced opening of the rhodamine spirocycle in sensor（1） led to the distinct colorimetric and fluorescence responses.Among all the tested ions,only Cr-（3＋） generated a significant fluorescence enhancement of up to13-fold,which indicated the high selectivity of 1.Sensor（1） was successfully applied in the in vivo fluorescence imaging of Cr-（3＋） in C.elegans.The results provided solid evidences for the future estimation of Cr-（3＋） in environmental applications and tobacco samples.