Objective:To screen the effect of 28 medicinal plants on inhibition of pancreatic lipase and evaluate the phytochemical contents of extracts.Methods:The ethanolic extracts of 28 traditional Thai herbal medicines were ...Objective:To screen the effect of 28 medicinal plants on inhibition of pancreatic lipase and evaluate the phytochemical contents of extracts.Methods:The ethanolic extracts of 28 traditional Thai herbal medicines were assayed for their in vitro activities against porcine pancreatic lipase using p-nitrophenyl butyrate as a substrate.Quantitative estimation of flavonoids,phenolics,and alkaloids was done.Results:Extracts from four herbs,Memecylon edule Roxb.,Garcinia vilersiana Pierre,Cryptolepis elegans Wall.and Phyllanthus chamaepeuce Ridl.,at a concentration of100 mg/m L,strongly inhibited porcine pancreatic lipase by 90.97%,92.04%,94.64%and95.38%,respectively.There was a significant positive correlation between phenolic content and inhibition activity.Inhibition activity was significantly correlated with flavonoid and with alkaloid contents.Conclusions:From this result,it could be concluded that herbs represent a rich of antipancreatic lipase compounds,in particular,Cryptolepis elegans Wall.and Phyllanthus chamaepeuce Ridl.It is suggested that the phytochemical compounds from these plants may be applied for the prevention and treatment of obesity or hyperlipidemia.展开更多
In this study,we investigated how Oroxylum indicum leaf and fruit extracts affect the viability and migration of MCF-7 breast cancer cells and the mechanisms of action responsible for these effects.MCF-7 cells treated...In this study,we investigated how Oroxylum indicum leaf and fruit extracts affect the viability and migration of MCF-7 breast cancer cells and the mechanisms of action responsible for these effects.MCF-7 cells treated with the extracts were examined using the sulforhodamine B,colony formation and caspase 3 activity assays,and by Western blotting.O.indicum extracts were found to inhibit MCF-7 cell growth in a concentration-and time-dependent manner,with 48 h IC50 values of 57.02±2.85μg/mL and 131.3±19.2μg/mL for leaf and fruit extracts,respectively.Further,the O.indicum leaf extract caused a reduction in MCF-7 cell viability,induction of MCF-7 cell apoptosis and ROS formation,and an increase in caspase 3 activity.Also,the two extracts inhibited MCF-7 cell migration and reduced both MMP 9 and ICAMP1 gene expression and MMP9 protein expression.Additionally,O.indicum extracts greatly reduced expression of the cell cycle regulatory protein Rac1 in the mevalonate pathway.In summary,O.indicum leaf and fruit extracts reduce breast cancer cell growth,cell viability and cell migration.O.indicum constituents could,therefore,be useful for augmenting the activity of chemotherapeutic drugs employed to treat breast cancer.展开更多
基金Supported by Ubon Ratchathani Rajabhat University through Plant Genetic Conservation Project Under The Royal Initiative of Princess Maha Chakri Sirindhorn Program(Grant No.UBRU_RSPG_2556)
文摘Objective:To screen the effect of 28 medicinal plants on inhibition of pancreatic lipase and evaluate the phytochemical contents of extracts.Methods:The ethanolic extracts of 28 traditional Thai herbal medicines were assayed for their in vitro activities against porcine pancreatic lipase using p-nitrophenyl butyrate as a substrate.Quantitative estimation of flavonoids,phenolics,and alkaloids was done.Results:Extracts from four herbs,Memecylon edule Roxb.,Garcinia vilersiana Pierre,Cryptolepis elegans Wall.and Phyllanthus chamaepeuce Ridl.,at a concentration of100 mg/m L,strongly inhibited porcine pancreatic lipase by 90.97%,92.04%,94.64%and95.38%,respectively.There was a significant positive correlation between phenolic content and inhibition activity.Inhibition activity was significantly correlated with flavonoid and with alkaloid contents.Conclusions:From this result,it could be concluded that herbs represent a rich of antipancreatic lipase compounds,in particular,Cryptolepis elegans Wall.and Phyllanthus chamaepeuce Ridl.It is suggested that the phytochemical compounds from these plants may be applied for the prevention and treatment of obesity or hyperlipidemia.
基金financially supported by Mahasarakham University(Fast Track 2019)。
文摘In this study,we investigated how Oroxylum indicum leaf and fruit extracts affect the viability and migration of MCF-7 breast cancer cells and the mechanisms of action responsible for these effects.MCF-7 cells treated with the extracts were examined using the sulforhodamine B,colony formation and caspase 3 activity assays,and by Western blotting.O.indicum extracts were found to inhibit MCF-7 cell growth in a concentration-and time-dependent manner,with 48 h IC50 values of 57.02±2.85μg/mL and 131.3±19.2μg/mL for leaf and fruit extracts,respectively.Further,the O.indicum leaf extract caused a reduction in MCF-7 cell viability,induction of MCF-7 cell apoptosis and ROS formation,and an increase in caspase 3 activity.Also,the two extracts inhibited MCF-7 cell migration and reduced both MMP 9 and ICAMP1 gene expression and MMP9 protein expression.Additionally,O.indicum extracts greatly reduced expression of the cell cycle regulatory protein Rac1 in the mevalonate pathway.In summary,O.indicum leaf and fruit extracts reduce breast cancer cell growth,cell viability and cell migration.O.indicum constituents could,therefore,be useful for augmenting the activity of chemotherapeutic drugs employed to treat breast cancer.
