Long noncoding RNAs(lncRNAs)have been confirmed to play a crucial role in various biological processes across several species.Though many efforts have been devoted to the expansion of the lncRNAs landscape,much about ...Long noncoding RNAs(lncRNAs)have been confirmed to play a crucial role in various biological processes across several species.Though many efforts have been devoted to the expansion of the lncRNAs landscape,much about lncRNAs is still unknown due to their great complexity.The development of high-throughput technologies and the constantly improved bioinformatic methods have resulted in a rapid expansion of lncRNA research and relevant databases.In this review,we introduced genome-wide research of lncRNAs in three parts:(i)novel lncRNA identification by high-throughput sequencing and computational pipelines;(ii)functional characterization of lncRNAs by expression atlas profiling,genome-scale screening,and the research of cancer-related lncRNAs;(iii)mechanism research by large-scale experimental technologies and computational analysis.Besides,primary experimental methods and bioinformatic pipelines related to these three parts are summarized.This review aimed to provide a comprehensive and systemic overview of lncRNA genome-wide research strategies and indicate a genome-wide lncRNA research system.展开更多
CD28 serves as a co-stimulator for T-cell activation and survival,and is expressed on all naive T cells in newborns.1 However,as T cells experience antigenmediated activation and differentiation,they gradually lose CD...CD28 serves as a co-stimulator for T-cell activation and survival,and is expressed on all naive T cells in newborns.1 However,as T cells experience antigenmediated activation and differentiation,they gradually lose CD28 expression.2 The age-related increase of CD28−T cells in human peripheral blood means that immune function declines in the elderly.1 Among the populations of CD28-T cells,CD8+CD28-T cells show the greatest age-related increase.1 These cells,which were first described in aged humans,exhibit weak immuneresponsiveness and shortened telomeres and are often considered to exhibit ageassociated replicative senescence.展开更多
基金supported by grants from the National Natural Science Foundation of China(No.82273158,81974436,81900559,and 31701116)the Natural Science Foundation of Guangdong Province,China(No.2022A1515010704,2022A1515011413,and 2021A1515010477)+2 种基金the Program for Guangdong Introducing Innovative and Entrepreneurial Teams(China)(No.2019ZT08Y485)Guangzhou Science and Technology Plan Projects(China)(No.202102020104)the Guangdong Province Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation(China)(No.2020B1212060018).
文摘Long noncoding RNAs(lncRNAs)have been confirmed to play a crucial role in various biological processes across several species.Though many efforts have been devoted to the expansion of the lncRNAs landscape,much about lncRNAs is still unknown due to their great complexity.The development of high-throughput technologies and the constantly improved bioinformatic methods have resulted in a rapid expansion of lncRNA research and relevant databases.In this review,we introduced genome-wide research of lncRNAs in three parts:(i)novel lncRNA identification by high-throughput sequencing and computational pipelines;(ii)functional characterization of lncRNAs by expression atlas profiling,genome-scale screening,and the research of cancer-related lncRNAs;(iii)mechanism research by large-scale experimental technologies and computational analysis.Besides,primary experimental methods and bioinformatic pipelines related to these three parts are summarized.This review aimed to provide a comprehensive and systemic overview of lncRNA genome-wide research strategies and indicate a genome-wide lncRNA research system.
基金supported by grant from the National Key Research and Development Program of China,Stem Cell and Translational Research(2017YFA0103403,2017YFA0105504)the National Natural Science Foundation of China(81425016,81600102,81601381,31771616,81730005)+3 种基金the Natural Science Foundation of Guangdong Province(S2013030013305,2016A030310131)the Key Scientific and Technological Projects of Guangdong Province(2014B020226002,2015B020228001,2015B020229001,2016A020214003,2016B030229002)Key Scientific and Technological Program of Guangzhou City(201508020262,201704020223)Guangdong Province Universities and Colleges Pearl River Scholar Funded Scheme(GDUPS,2013).
文摘CD28 serves as a co-stimulator for T-cell activation and survival,and is expressed on all naive T cells in newborns.1 However,as T cells experience antigenmediated activation and differentiation,they gradually lose CD28 expression.2 The age-related increase of CD28−T cells in human peripheral blood means that immune function declines in the elderly.1 Among the populations of CD28-T cells,CD8+CD28-T cells show the greatest age-related increase.1 These cells,which were first described in aged humans,exhibit weak immuneresponsiveness and shortened telomeres and are often considered to exhibit ageassociated replicative senescence.
