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Molecular cloning of rice cDNAs related to pollen development using the subtraction hybridization technique 被引量:1
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作者 Zee S.Y Yau C.P. 《Chinese Science Bulletin》 SCIE EI CAS 1999年第20期1898-1903,共6页
The meiotic stage of pollen mother cell is a very important stage in controlling the development and formation of pollen. In order to clone the rice cDNA(s) of this stage, a normal rice, Annong N and its thermosensiti... The meiotic stage of pollen mother cell is a very important stage in controlling the development and formation of pollen. In order to clone the rice cDNA(s) of this stage, a normal rice, Annong N and its thermosensitive mutant, Annong S-1 were used as the plant material. The mRNA has been extracted from the young panicle at the meiotic stage. By using the cDNA subtraction hybridization technique, three cDNA fragments, RP-1, RP-2 and RP-3 have 展开更多
关键词 cDNA SUBTRACTION HYBRIDIZATION POLLEN development rice.
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