Melanin involves in various biological functions, widely used in cosmetic, pharmacology medicine and other fields. However, melanin application is limited due to low productivity and high cost. In this work, melanin e...Melanin involves in various biological functions, widely used in cosmetic, pharmacology medicine and other fields. However, melanin application is limited due to low productivity and high cost. In this work, melanin extraction method from rapeseed meal(RSM) was explored. Effects of hydrochloric acid(HCl) concentration, ethanol concentration, extraction temperature and time, and solid-liquid ratio were evaluated systematically. According to results of single factor experiment and Box-Behnken experiment, the optimum extraction conditions of melanin from RSM were as follows: HCl concentration, 0.5 mol/L; ethanol concentration, 70%; extraction temperature, 40 o C; extraction time, 1 h; solid liquid ratio, 1/4 g/mL. Under these conditions, extraction yields of crude melanin(CM) and purified melanin(PM) were 9.00% and 1.44%, respectively. Compared with synthetic melanin(SM) from Sigma, the relative purities of CM and PM were 7.82% and 29.57%, respectively. Moreover, feedstuff value of RSM residue was also improved after melanin isolation by significantly reducing glucosinolate content.展开更多
Dear Editor When membrane proteins and secretory proteins are incom- pletely folded or mis-folded, they are retained in the endo- plasmic reticulum （ER） for further folding or degradation. Two major degradation syst...Dear Editor When membrane proteins and secretory proteins are incom- pletely folded or mis-folded, they are retained in the endo- plasmic reticulum （ER） for further folding or degradation. Two major degradation systems involved in removing the mis-folded or unfolded proteins retained in the ER have been identified： ER-associated degradation （ERAD）, which targets misfolded proteins for ubiquitination and subsequent degradattion through the proteasome pathway （McCracken and Brodsky, 1996）.展开更多
基金supported by Ministry of Science and Technology of the People’s Republic of China (2016YFD0501209)Chinese Academy of Agricultural Sciences (CAAS-ASTIP-2013-OCRI)
文摘Melanin involves in various biological functions, widely used in cosmetic, pharmacology medicine and other fields. However, melanin application is limited due to low productivity and high cost. In this work, melanin extraction method from rapeseed meal(RSM) was explored. Effects of hydrochloric acid(HCl) concentration, ethanol concentration, extraction temperature and time, and solid-liquid ratio were evaluated systematically. According to results of single factor experiment and Box-Behnken experiment, the optimum extraction conditions of melanin from RSM were as follows: HCl concentration, 0.5 mol/L; ethanol concentration, 70%; extraction temperature, 40 o C; extraction time, 1 h; solid liquid ratio, 1/4 g/mL. Under these conditions, extraction yields of crude melanin(CM) and purified melanin(PM) were 9.00% and 1.44%, respectively. Compared with synthetic melanin(SM) from Sigma, the relative purities of CM and PM were 7.82% and 29.57%, respectively. Moreover, feedstuff value of RSM residue was also improved after melanin isolation by significantly reducing glucosinolate content.
文摘Dear Editor When membrane proteins and secretory proteins are incom- pletely folded or mis-folded, they are retained in the endo- plasmic reticulum （ER） for further folding or degradation. Two major degradation systems involved in removing the mis-folded or unfolded proteins retained in the ER have been identified： ER-associated degradation （ERAD）, which targets misfolded proteins for ubiquitination and subsequent degradattion through the proteasome pathway （McCracken and Brodsky, 1996）.
