Implantation Instrument on the Expression of Collagen-I and Cell Proliferation Markers in Pathological Scar

Objective To explore the efficacyof Scar Infusion Instrument on expression of Type I Collagen and Cell Proliferation Markers in Pathological Scars.Methods 70 cases patients with pathologic scar admitted in our hospital from Augst 2016 to July 2017 were randomly divided into study group and control group.The control group was treated with scar ointment.The treatment group was treated with scar ointment combined with scar The expression of COL-I in scar tissue was determined by enzyme-linked immunosorbent assay(ELISA).The expression of ki-67 nuclear protein was determined by immunohistochemical staining(sp).Results The efficacy of the study group was significantly better than the control group,the total efficiency of the study group was significantly higher than the control group after 6 months treatment(P<0.05);The average COL-I and Ki67 nuclear protein expression in the single slice of the study group was significantly lower than that of the control group at the 3th months,6th months after treatment(P<0.001).Conclusions The scarring device is more effective in the treatment of pathological scar,and its mechanism may be related to the better inhibition of COL-I and ki-67 nucleoprotein expression.

Optimized liver resection range and perioperative safety in patients with high levels of indocyanine green R15

The indocyanine green(ICG)clearance test is an objective measurement of functional liver reserve(FLR)[1].The liver can preserve normal functions with a 70%−80%functional liver volume resection[2]and the remnant is capable of regeneration.However,liver regenerative properties are affected by hepatitis,fatty liver,cirrhosis and damage caused by chemotherapy[3].ICG combined with the Child-Pugh score are main criteria for evaluating FLR.A safe hepatectomy procedure requires the remnant liver to be 25%−30%of the normal volume.For patients with an indocyanine green retention rate at 15 min(ICG-R15)>20%,segment liver resection,limited hepatectomy or tumor enucleation is recommended,but there has been no clear clarification of the essential remnant liver volume[4].In China,the experts’consensus of preoperative evaluation of liver reserve function incorporates ICGR15 tests.

Ginsenoside Rd improves behavioral impairment of rats with acute plateau status by modulating synaptic plasticity

Objective:To investigate the protective effect of ginsenoside Rd on the improvement of the behavior and synaptic plasticity in rats with acute plateau status.Methods:A total of 60 Wistar rats were randomly divided into the control group,the model group,and the intervention group,with 20 rats in each group.The model was established in low-pressure oxygen chamber simulating the plateau,and the intervention group was administered with ginsenoside.Electron microscope was used to observe synaptic ultrastructure of hippocampal CA1 area,and analyze the structural parameters on the Gray I synaptic interface.Morris water maze and Y electric maze experiment were used for behavioral detection.Results:Compared with the control group,the number of electrical stimulation required for rat to avoid was increased in the model group,the latency in the Morris water maze was prolonged,the swimming distance was increased,and the frequency of crossing the platform was decreased.Under the electron microscope,the synaptic cleft was increased,the length of the synaptic active area was shorter,the postsynaptic density(PSD)was thinner,the flat synapse was increased,and the concave and perforated types were significantly reduced.Compared with the model group,the number of electrical stimulation required for rat to avoid was decreased in the intervention group,the latency in the Morris water maze was shortened,the swimming distance was decreased,and the frequency of crossing the platform was increased.Under the electron microscope,the synaptic cleft was decreased,PSD was thicker,the flat synapse was decreased,and the concave and perforated types were increased.Conclusion:Low pressure and low oxygen environment of plateau damages the plasticity changes of the synaptic structure and function.And to a certain extent,ginsenoside Rd reverses Gray I synaptic interface structure parameters,so as to improve the behavior performance of model rats at high altitude condition.