Medication Rules of Traditional Chinese Medicine for Treating Threatened Abortion in the Early Stage of Pregnancy with Subchorionic Haematoma Based on Data Mining

Objective:Using data mining technology to explore the rules of traditional Chinese medicine(TCM)in the treatment of threatened abortion in the early stage of pregnancy with sub-chorionic haematoma(SCH).Methods:Literature of TCM in the treatment of threatened abortion in the early stage of pregnancy with SCH were retrieved from CNKI,VIP,WANFANG and Pubmed,EMBASE.The literature information database was established to be used for descriptive analysis,association rule analysis and cluster analysis of relevant data.Results:A total of 100 literatures were included,involving 114 Chinese herbs.The efficacy of Chinese herbs were mainly tonic drugs,hemostatic drugs,heat-clearing drugs,dissolving blood stasis and hemostatic drugs.The medicinal properties were mostly mild and warm,and the taste of the drug was mainly sweet,bitter and pungent.The liver meridian,spleen meridian and kidney meridian were frequently used.The commonly used drug pair combination was"Xu duan(Radix dipsaci,续断)-Tusizi(Semen Cuscutae,菟丝子)",and the core combination was"Tusizi-Xu duan-Ejiao(Donkeyhide gelatin,阿胶)-Baizhu(Atractylodes macrocephala,白术)-Dangshen(Codonopsis pilosula,党参)".Commonly used drugs for removing blood stasis and hemostasis were with Sanqi(Panax notoginseng,三七),Puhuang(cattail pollen,蒲黄),and Qiancao(Radix Rubiae,茜草).Conclusion:Data mining traditional Chinese medicine for the treatment of threatened abortion in the early stage of pregnancy with SCH clinically commonly used drug efficacy,taste,meridian,commonly used drug pairs,core combination and commonly used blood stasis hemostatic drugs,has important reference significance for the treatment of threatened abortion in the early stage of pregnancy combined with SCH.

Effect of synoviectomy combined with postoperative radiotherapy on pigmented villonodular synovitis in knee:A meta-analysis

Objective:To evaluate the efficacy of synovectomy combined with postoperative radiotherapy in the treatment of pigmented villonodular synovitis compared with only synovectomy in knee. Methods:The related references were searched from SinoMed,CNKI,Wanfang,VIP,PubMed,Embase,Cochrane Library and Web of Science from date of databases establishment to October 2020,and the studies of synovectomy combined with postoperative radiotherapy were screened. According to the type of research,the corresponding evaluation methods were selected for quality evaluation of the literature. RevMan 5.3 software was used for meta-analysis. The primary outcome was recurrence,while the secondary outcomes included complications,excellent and good rate of function of knee,range of motion of knee,Lysholm score,and IKDC score of knee etcetera. Results:A total of 11 articles were included in meta-analysis,with 510 cases,280 in the combined group and 230 in the control group. The results of meta-analysis showed that:(1)The recurrence rate in the combination group was significantly lower than that in the control group,[OR=0.33,95%CI(0.20,0.55),P<0.000 1];(2)The complications in the combined group were higher than that in the control group[OR=2.70,95%CI(1.16,6.31),P=0.02];(3)The excellent and good rate of the combined group was significantly better than that of the control group[OR=2.86,95%CI(1.23,6.64),P=0.01],and it also may be the result of different evaluation criteria of each study;(4)There was no significant difference range of motion[MD=2.96,95%CI(-0.54,6.47),P=0.10],Lysholm score[MD=3.22,95%CI(-0.51,6.96),P=0.09],or IKDC score[MD=2.88,95%CI(-1.07,6.82),P=0.15]in knee between the two groups. Conclusion:The meta-analysis shows that synovectomy combined with radiotherapy can reduce the recurrence rate of pigmented villonodular synovitis in knee,but compared with only operation,there are higher complications. However,the above conclusions need to be verified by long-term follow-up of more high-quality clinical studies.

Relationship between peripheral blood neutrophil/lymphocyte ratio and the prognosis of patients with brittle femoral neck fracture

Objective:To explore the relationship between peripheral blood neutrophil/lymphocyte ratio(NLR)and osteoporotic femoral neck fractures and to evaluate the prognosis.Methods:The subjects were 102 patients who received osteoporotic femoral neck fracture in the First Affiliated Hospital of Guangzhou University of Chinese Medicine from 2008 to 2017.Patients were divided into three groups according to the BMD values in the diagnostic criteria of primary osteoporosis:normal bone mass group(T≥-1.0),reduced bone mass group(-2.5<T<-1.0)and osteoporosis group(T≤-2.5).In addition,during postoperative follow-up,patients receiving internal fixation were divided into the high NLR group(NLR>2.33)and the low NLR group(NLR≤2.33)according to their NLR values.The clinical data of patients with osteoporotic femoral neck fracture who underwent peripheral blood routine examination were prospectively analyzed.The statistical indicators included age,gender,fracture cause and other general conditions,fracture type,peripheral blood neutrophil/lymphocyte ratio,bone mineral density(BMD)level and blood lipid level,and prognosis of 102 patients who were followed up for more than 2 years after surgery.The relationship between peripheral blood NLR and the above indexes was observed.Statistical methods including normal data analysis of variance between groups,SNK-q test is compared between two groups,the partial correlation analysis between the two factors,the relationship between multiple factors using multi-factor linear regression analysis,and normal data comparison between the two groups using t test,two sets of independent data frequency distribution of binary classification variables compared by chi-square test.Moreover,NLR is also a major factor affecting the prognosis and rehabilitation of osteoporotic femoral neck fractures.Results:The analysis of variance between the two groups(SNK-q test of the comparison between the two groups),single-factor partial correlation analysis,multi-factor linear regression analysis showed that compared with the normal group and the reduced group,the levels of age,triglyceride and NLR in the osteoporosis group were increased,while BMD was significantly decreased.BMD in patients with osteoporotic femoral neck fracture was negatively correlated with NLR and triglyceride(P<0.05).In multi-factor linear regression analysis,NLR and triglyceride were the main influencing factors of BMD in patients with osteoporotic femoral neck(P<0.05).In addition,increased peripheral blood NLR in patients treated with internal fixation may increase the risk of postoperative femoral head necrosis,nonunion of the broken end of the fracture,and prolong the time of postoperative functional recovery.Conclusion:The level of peripheral blood NLR in patients with osteoporotic femoral neck fracture is increased and correlated with bone mineral density,which suggested that peripheral blood NLR can be used as one of the clinical indicators for early screening and prevention of osteoporotic femoral neck fracture.In addition,peripheral blood NLR is also a major factor affecting the prognosis and rehabilitation of osteoporotic femoral neck fractures.

Bibliometrics of stem cell effects on aging in the last decade based on the Web of Science database

Objective:To summarize the recent 10 years of related literature on stem cell effect on aging and explore the research results and hot topics.Methods:Based on the core databaseof Web of Science,all relevant literature from January 1,2012,to December 31,2021,was screened,and CiteSpace 6.1.R2 software was used to show the intrinsic value of literature.The number of publications,research institutions,research countries,co-cited literature,and keywords was analyzed and visualized.Results:In the past 10 years,the total number of articles published showed an upward trend.The number of articles published by relevant countries was 3,911,and the United States had the most significant number of articles published and the highest centrality.The research institutions were mainly universities,supplemented by research centers,and the quality of articles published by famous foreign universities in this field was high.The most co-cited literature is“Naturally occurring p16(Ink4a)-positive cells shorten healthy lifespan”by Darren J Baker,which clarifies the feasibility of cell therapy for aging.The high-frequency keywords are stem cell,senescence,expression,etc.,and the most central ones are cancer cell,migration and DNA repair,etc.In addition,emergent words and clustering show that research hotspots are mainly in the aging mechanism.There are many research entry points,and the pathogenesis and development of senescence,therapeutic targets of ion channels in senescent cells,and their effects on exosomes are the main focus of current research.Conclusion:Literature through the analysis of 10 years of research literature summary suggests that stem cell function in aging still needs further study;the United States is in the leading position in this regard,and the research results also show that research institutions dominate in the developed countries,North America and Europe in the increasing number of papers at the same time,and China should pay attention to the quality of the post,in order to make more progress.The literature of some high-quality scholars can be used as a reference to expand our thinking.The aging mechanism still needs to be many follow-upstudies to clarify the pathway and target in the treatment of more refined Alzheimer’s disease and other age-related diseases need to delve into.Subsequent scholars should study the specific role of stem cells in treating aging;in recent years,hot words,such as exosomes,oxidative stress,etc.,can be used as the thinking of the follow-up study and contribute to the development of the field.

Mechanism of "Pinelliae rhizoma-Arisaematis rhizoma" in treatment of lung cancer based on network pharmacology

Objective:The study was designed to explore the mechanism of“Pinelliae RhizomaArisaematis Rhizoma”(PR-AP)in treatment of lung cancer based on network pharmacology.Methods:The active components and their targets of“Pinelliae Rhizoma-Arisaematis Rhizoma”were screened out from TCMSP database.The cytoscape3.7.0 software was used to construct the drug-active component-targets network.Searched the DisGeNet database to obtain lung cancer related targets.Bisogenet was used to construct"PPI network of active component target"and"PPI network of lung cancer target",and the intersection of the two networks was taken and the target was screened.AutoDockTools software was used to dock the key active ingredients with the key targets.David database was used to perform Go biological process enrichment analysis and KEGG pathway enrichment analysis.Results:A total of 15 active components were collected,including beta-sitosterol,baicalein,Stigmasterol,Cavidine,coniferin,etc.These active components mainly act on 95 key targets such as ESR1 and CDK2,which enriched in multiple lung cancer related signaling pathways,such as PI3KAkt、MAPK、HIF-1、FoxO、TGF-β、Hippo、TNF、Notch、VEGF、cAMP,etc.The results of molecular docking showed that the active components could regulate the core targets(ESR1 and CDK2).Conclusion:The effect of“Pinelliae Rhizoma-Arisaematis Rhizoma”in the treatment of lung cancer embodies the characteristics of multi-component,multi-target and multi-pathway of traditional Chinese medicine.Its mechanism of action may be to play a role in the treatment of lung cancer by regulating cell cycle,angiogenesis,tumor stem cells,etc.This research can provide ideas and references for further research.

Professor Zhang Yu-zhen's medication rule in treating metrorrhagia and bleeding based on data mining

Objective:To explore the medication rules of Professor Zhang Yuzhen in treating the bleeding period of metrorrhagia and metrostaxisthe,and to explore the treatment of the disease.Methods:Collected 520 prescriptions of Professor Zhang Yuzhen in the treatment of patients with the bleeding period of metrorrhagia and metrostaxis,and analyzed the regularity of medication by using"TCM inheritance assist platform(V2.5)".Results:According to the analysis,the top 30 most frequently used herbs for treating the bleeding period of metrorrhagia and metrostaxisthe were Codonopsis pilosula,Trogopterus dung,Cattail pollen,Motherwort,Cornus,Ophiopogon japonicas,Schisandra,Tortoiseshell,Panax notoginseng,Radix Paeoniae Alba,etc.Core combination 35 In the group,the core prescription has 1 hemostasis,followed by 2 hemostasis,and evolves to get 3 new prescriptions.Conclusion:Professor Zhang Yuzhen's medication for the treatment of metrorrhagia and metrostaxisthe is mostly Gan Wen's product.The treatment is based on the three organs of the liver,spleen,and kidney,and the disease is treated with"tonifying the kidney and benefiting qi,reducing blood stasis and stopping bleeding".

Fu-Zheng-Yi-Liu Formula inhibits the stem cells and metastasis of prostate cancer via tumor-associated macrophages/C-C motif chemokine ligand 5 pathway in tumor microenvironment

Prostate cancer(PCa)is the second most common malignancy among men globally.The Fu-Zheng-Yi-Liu(FZYL)Formula has been widely utilized in the treatment of PCa.This study investigates whether the FZYL Formula can inhibit PCa by tar-geting the TAMs/CCL5 pathway.We conducted in vitro co-cultures and in vivo co-injections of PCa cells and TAMs to mimic their in-teraction.Results showed that the FZYL Formula significantly reduced the proliferation,colony formation,subpopulations of PCSCs,and sphere-formation efficacy of PCa cells,even in the presence of TAM co-culture.Additionally,the Formula markedly decreased the migration,invasion,and epithelial-mesenchymal transition(EMT)of PCa cells induced by TAMs.The FZYL Formula also reversed M2 phenotype polarization in TAMs and dose-dependently reduced their CCL5 expression and secretion,with minimal cytotoxicity observed.Mechanistic studies confirmed that the TAMs/CCL5 axis is a critical target of the FZYL Formula,as the addition of exogen-ous CCL5 partially reversed the formula’s inhibitory effects on PCSCs self-renewal in the co-culture system.Importantly,the Formula also significantly inhibited the growth of PCa xenografts,bone metastasis,and PCSCs activity in vivo by targeting the TAMs/CCL5 pathway.Overall,this study not only elucidates the immunomodulatory mechanism of the FZYL Formula in PCa therapy but also highlights the TAMs/CCL5 axis as a promising therapeutic target.

Thymosin Alpha-1 Inhibits Complete Freund’s Adjuvant-Induced Pain and Production of Microglia-Mediated Pro-inflammatory Cytokines in Spinal Cord

Activation of inflammatory responses regulates the transmission of pain pathways through an integrated network in the peripheral and central nervous systems.The immunopotentiator thymosin alpha-1(Tal)has recently been reported to have anti-inflammatory and neuroprotective functions in rodents.However,how Tα1 affects inflammatory pain remains unclear.In the present study,intraperitoneal injection of Tal attenuated complete Freund's adjuvant(CFA)-induced pain hypersensitivity,and decreased the up-regulation of pro-inflammatory cytokines(TNF-α,IL-1β,and IL-6)in inflamed skin and the spinal cord.We found that CFA-induced peripheral inflammation evoked strong microglial activation,but the effect was reversed by Tα1.Notably,Tα1 reversed the CFA-induced up-regulation of vesicular glutamate transporter(VGLUT)and down-regulated the vesicular γ-aminobutyric acid transporter(VGAT)in the spinal cord.Taken together,these results suggest that Tα1 plays a therapeutic role in inflammatory pain and in the modulation of microgliainduced pro-inflammatory cytokine production in addition to mediation of VGLUT and VGAT expression in the spinal cord.

Adjunctive melatonin for tardive dyskinesia in patients with schizophrenia： a meta-analysis

背景:迟发性运动障碍(TD)的临床特征是异常不自主运动。TD具有严重的不可逆的致残性和社会功能损害。目的 :此荟萃分析基于随机对照试验(RCTs)文献系统评估褪黑素对精神分裂症患者迟发性运动障碍的临床疗效和安全性。方法 :两位独立评估者从以下数据库对相关的临床随机对照试验(RCT)文献进行检索(万方数据、中国知网(CNKI)、中国生物医学文摘数据库和PubM ed、PsycI NFO、Embase、Cochrane Library数据库),检索时间截止于2017年6月8日。以TD症状严重程度为主要结局指标,采用Rev Man 5.3版本进行统计分析,对RCTs的质量评估采用Cochrane风险评估偏倚和Jadad量表来评估各种偏倚的风险性。采用GRADE(Grades of Recommendation,Assessment,Development,and Evaluation)系统推荐分级方法对meta-分析结果的整体证据质量水平进行分级评价。结果 :最终筛选确定4个RCTs(n=130)。3个RCTs采用双盲法,1个RCT单盲,根据Cochrane风险评估偏倚和Jadad量表显示3个RCTs的疗效评估指标的证据质量被评定为"高质量"。与对照组相比,根据不自主运动量表(AIMS)评定褪黑素可改善TD严重程度(4个RCTs,n=130,加权平均差值(WMD):-1.52(95%CI:-3.24,0.20),p=0.08;I2=0%),但尚没有达到显著差异。根据等级方法,改善TD症状的meta分析结果的整体证据质量被评为"低",而关于不良反应和认知损害方面则数据太少。结论 :荟萃分析表明,褪黑素或可改善精神分裂症TD症状。但仍有待今后更高质量和更大样本的RCTs验证。

Acute Cerebellar Infarction Complicated with Multiple Intracerebral Hemorrhage Treated by An Integrated Chinese and Western Medicine Approach:A Case Report

Cerebellar infarction accounts for almost 3%of all ischemic strokes,with 27,400 new cases each year in the USA alone.（1）Since the computerized tomography（CT）scan is often negative in the early hours after onset,it is difficult to diagnose,

对精神分裂症患者血脂异常的瑞舒伐他汀辅助治疗的疗效与安全性的的Meta分析（英文）

背景:精神分裂症患者的代谢综合征是一个重要的健康问题。瑞舒伐他汀对血脂异常的辅助性治疗的有效性和安全性存在争议。目的:评价瑞舒伐他汀对精神分裂症患者血脂异常的辅助性治疗的有效性和安全性。方法:我们从以下数据库中系统地检索了2017年9月28日以前相关的临床对照试验:Pub Med、Psyc INFO、Cochrane图书馆、中国知网、万方数据库、中国生物医学文献数据库。我们计算了标准平均差(SMD)、风险比(RR)及其95%的可信区间(CIs)。使用偏移评估工具中的Cochrane风险评估来评价所纳入研究的质量。并采用GRADE系统推荐的等级方法(推荐、评估、发展、评价的等级)作为参照标准。结果:确认和分析了4项比较瑞舒伐他汀组(n=138)和对照组(n=136)的研究(n=274)。瑞舒伐他汀辅助治疗显示对低密度脂蛋白胆固醇(LDL-C)[4项试验,n=272,SMD:-1.31(95%CI:-1.93,-0.70),I^2=81%]、总胆固醇(2项试验,n=164,SMD:-2.00(95%CI:-2.79,-1.21);I^2=76%)、和甘油三酯(2项试验,n=164,SMD:-1.05(95%CI:-1.38,-0.72);I^2=0%)的疗效比对照组更有效,但对高密度脂蛋白胆固醇(2项试验,n=164,SMD:0.14(95%CI:-0.16,0.45);I^2=0%)的疗效没有显著差异。去除一项没有随机试验的LDL-C研究之后,显著差异仍然存在[3项试验,n=172,SMD:-1.07(95%CI:-1.60,-0.53);I^2=63%]。体重(3项试验,n=208,SMD:-0.40(95%CI:-1.29,0.49);I^2=89%)、身体质量指数(2项试验,n=164,SMD:-0.34(95%CI:-1.23,0.56);I^2=87%)、腰围(3项试验,n=208,SMD:-0.43(95%CI:-1.31,0.46);I^2=89%)、空腹血糖(4项试验,n=272,SMD:-0.25(95%CI:-0.65,0.15);I^2=62%)方面组间没有显著的差异。两组之间的不良反应及停药率相近。根据GRADE分级方法,主要结果的证据水平低被评为"非常低"(35.3%)到"低"(64.7%)。其中,主要结果(LDL-C)被评为"非常低"。结论:对精神分裂症患者血脂异常的瑞舒伐他汀辅助性治疗的现有数据尚不足以对其有效性和安全性做出明确的解释。需进一步针对高质量的延长治疗时间的随机对照试验来验证该结论。

Leech-Centipede Granules Suppress EndMT to Improve Erectile Dysfunction in Rats with Diabetes Mellitus via TGF-β/Smad Pathway

Objective:To investigate whether Leech-Centipede(LC) Granules can improve erectile function in rats with diabetes mellitus-associated erectile dysfunction(DMED) through endothelial-to-mesenchymal transition(EndMT) inhibition.Methods:Components of LC Granules were identified via ultra-high-performance liquid chromatography.Thirty male Sprague Dawley rats were injected with streptozotocin and fed continuously for 8weeks to establish the DMED rat model.Rats with erectile dysfunction symptoms diagnosed using apomorphine were divided into DMED and low-,medium-,and high-doses LC groups(n=6 in each).The negative control(NC, n=6) and DMED groups were given 5 mL of deionized water via intragastric gavage,and the low-,mediumand the high-doses LC groups were administered LC at 1.6,3.2,and 6.4 g/kg,respectively,via intragastric gavage for 4 weeks.The intracavernous pressure(ICP),mean arterial pressure(MAP),and nitric oxide(NO) levels in cavernous tissue were measured for each group.Quantitative reverse transcription-polymerase chain reaction and Western blot were used to detect mRNA and protein expressions of endothelial and mesenchymal markers.Immunofluorescence staining was used to observe α-SMA, and Masson’s trichrome staining was performed to determine the myofiber/collagen ratio.Results:A total of 474 active components were identified.After treatment,the ICP/MAP value and NO level were significantly higher in the medium-and high-dose LC groups than in the DMED group(P<0.05).Compared with the DMED groups,the medium-and high-dose groups LC significantly increased and decreased endothelial and mesenchymal markers expression,respectively(P<0.05).Tumor growth factor(TGF) β RⅡ,p-Smad2, and p-Smad3 levels were considerably higher following diabetes onset but reduced following LC intervention(P<0.05),except for TGF β 1(P>0.05).α-SMA expression was significantly higher in the DMED group and was reduced in all LC intervention groups(P>0.05).The myofiber/collagen ratio in the LC groups was higher than that in the DMED group but lower than that in the NC group(all P<0.05).Conclusions:LC Granules may improve the erectile function of DMED rats by suppressing TGF-β/Smad pathway to reverse EndMT.