A REPORT ON FOUR CASES OF LIVER CARCINOMA TREATED BY TOPICAL ADHESIVE METHOD

Liver carcinoma has been one of themalignant tumors of common occurrenceand high mortality in China,and cases ofmiddle and late period are very difficult tobe treated.However,the present authorshave improved the condition of patientswith liver carcinoma and prolonged theirsurvival period by a topical adhesive methodas the chief therapeutic means.The

A rat pup model of cerebral palsy induced by prenatal inflammation and hypoxia

Animal models of cerebral palsy established by simple infection or the hypoxia/ischemia method cannot effectively simulate the brain injury of a premature infant. Healthy 17-day-pregnant Wistar rats were intraperitoneally injected with lipopolysaccharide then subjected to hypoxia. The pups were used for this study at 4 weeks of age. Simultaneously, a hypoxia/ischemia group and a control group were used for comparison. The results of the footprint test, the balance beam test, the water maze test, neuroelectrophysiological examination and neuropathological examination demonstrated that, at 4 weeks after birth, footprint repeat space became larger between the forelimbs and hindlimbs of the rats, the latency period on the balance beam and in the Morris water maze was longer, place navigation and ability were poorer, and the stimulus intensity that induced the maximal wave amplitude of the compound muscle action potential was greater in the lipopolysaccharide/hypoxia and hypoxia/ischemia groups than in the control group. We observed irregular cells around the periventricular area, periventricular leukomalacia and breakage of the nuclear membrane in the lipopolysacchadde/hypexia and hypoxia/ischemia groups. These results indicate that we successfully established a Wistar rat pup model of cerebral palsy by intraperitoneal injection of lipopolysaccharide and hypoxia.

Clinical significance of costimulatory molecule B7-H3 expression on CD3＋T cells in colorectal carcinoma

Background B7-H3 has been widely studied in the context of tumor progression in recent years,and behaves as a tumor cell marker in a variety of tumors including colorectal carcinoma.The mechanism of B7-H3 in tumor progression is complicated and not clear yet.Studies have revealed that B7 family molecules are expressed on infiltrated lymphocytes as well as tumor cells in tumor microenvironment,which indicates that different expression pattern may lead to different clinical outcomes.Methods The expression of B7-H3 was detected in tissues of 98 colorectal carcinoma patients by using immunohistochemistry.Then the expression of B7-H3 on CD3＋ T lymphocytes isolated from fresh cancer tissues of 12 colorectal carcinoma patients was analyzed by flow cytometry assay.The relationship between the expression of B7-H3 on CD3＋ T lymphocytes and patients＇ clinical pathological parameters was demonstrated with statistical analysis.Results Patients with more CD3＋ T cell infiltration survived much longer than patients with less CD3＋ T cell infiltration （P ＜0.05）; B7-H3 was highly expressed by infiltrating CD3＋ T lymphocytes in colorectal carcinoma tissues.The expression of B7-H3 was found to be significantly related with lymph node metastasis status （P ＜0.05）,but not with the patient＇s gender,age,tumor size,differentiation degree,depth of tumor invasion,Dukes＇ stage,distant metastasis and whether or not mucinous adenocarcinoma was present （P ＞0.05）.Moreover,the survival time of patients with low expression of B7-H3 was obviously longer than those of high B7-H3 expression patients,but the seven-year survival rate showed no difference between the high and low B7-H3 expression patients （P ＞0.05）.Conclusion The negative costimulatory molecule B7-H3 on infiltrating CD3＋ T lymphocytes in colorectal carcinoma bears importance in the clinical pathological progress and prognosis of colorectal carcinoma.