Rats(Sprague Dawley,200-300g)wereoperated under anesthesia with nembuta1.Afterthe laminectomy,20-25%aqueous solution ofHRP(Sigma 6)was injected unilatera11y intothe spinal cord at cervical or lumbar leve1.Theanimals w...Rats(Sprague Dawley,200-300g)wereoperated under anesthesia with nembuta1.Afterthe laminectomy,20-25%aqueous solution ofHRP(Sigma 6)was injected unilatera11y intothe spinal cord at cervical or lumbar leve1.Theanimals were allowed to survive for 36-48hours and transcardically perfused with 0.9%NaCl,3.5%paraforma1dehyde,and 10%sucr-ose in 0.1mol/L PB.The brain was removed andimmersed in 10%sucrose at 4℃.展开更多
Ju Gong reported in 1981 that HRP could be anterogradely transported for a long distance by neurons. Based on this fact the rate of anterograde transport was investigated and at the same time the rate of retrograde tr...Ju Gong reported in 1981 that HRP could be anterogradely transported for a long distance by neurons. Based on this fact the rate of anterograde transport was investigated and at the same time the rate of retrograde transport and the time of disappearance of HRP in the labeled sites were observed. Twenty-two rabbits weighing 2—2.2 kg were used, HRP (30%, 1.2μ1) was injected unilaterally into the L6 spinal cord. The animals were decapitated 12 hr to 7 days after the injection and their brains were processed with o-dionisidine. Labeling in the brain stem was then searched for. The ante-展开更多
In the hypothalamus there are many cells known to terminate in the posterior pituitary gland, which are mainly vasopressinergic and oxytocinergic neurons. Immunohistochemical studies have shown that they are concentra...In the hypothalamus there are many cells known to terminate in the posterior pituitary gland, which are mainly vasopressinergic and oxytocinergic neurons. Immunohistochemical studies have shown that they are concentrated in the supraoptic nucleus (SON) and the paraventricular nucleus (PVN) also found to be scattered in展开更多
The centrifugal control of exteroceptors, particularly the efferent influence of sympathetic nerves on receptors has been well documented. Loewenstein and Chernetski observed that the activity of tactile receptors of ...The centrifugal control of exteroceptors, particularly the efferent influence of sympathetic nerves on receptors has been well documented. Loewenstein and Chernetski observed that the activity of tactile receptors of the skin could be facilitated by sympathetic nerves. Santini et al. recently confirmed that the sympathetic nerves were distributed to the vicinity of sensory nerve endings, and the afferent展开更多
With the progress of the horseradish peroxidase (HRP) technique, some new connections between the spinal cord and the brain have been discovered. After injecting HRP into the spinal cord of the cat, we have also found...With the progress of the horseradish peroxidase (HRP) technique, some new connections between the spinal cord and the brain have been discovered. After injecting HRP into the spinal cord of the cat, we have also found some connections hitherto undescribed or not fully described. The HRP is generally used as a retrograde tracer. Some short-distance HRP anterograde tracing studies have also been published.展开更多
Most authors believed that the spinal cord was not directly connected with the hypothalamus. However, direct hypothalamo-spinal projection has been discovered recently in several species by means of the horseradish pe...Most authors believed that the spinal cord was not directly connected with the hypothalamus. However, direct hypothalamo-spinal projection has been discovered recently in several species by means of the horseradish peroxidase tracing technique. No unequivoeal evidence of direct spino-hypothalamic projection is available. Seven rabbits were used in the present study. 30％ aqueous horseradish peroxidase (Sigma Ⅵ)展开更多
Most of the reports on human placental villi endocrinology focus on the study of neuropeptides, pituitary-or gonadal-like hormones, growth factors and cytokines, and only a few of them concern the function of biogenic...Most of the reports on human placental villi endocrinology focus on the study of neuropeptides, pituitary-or gonadal-like hormones, growth factors and cytokines, and only a few of them concern the function of biogenic amine. We first discovered that trophoblast of placental villi can produce 5-hydroxytryptamine (5-HT) and that its展开更多
A new time-resolved fluorimetric method for the measurement of natural killer (NK) cell cytotoxicity has been developed by labelling the target cell K562 with a new synthesized fluorescence marker KLUK. The method has...A new time-resolved fluorimetric method for the measurement of natural killer (NK) cell cytotoxicity has been developed by labelling the target cell K562 with a new synthesized fluorescence marker KLUK. The method has advantages of higher sensitivity, time-saving, good reproducibility and has no radioactivity problems. A satisfactory result is obtained by comparing it with 51Cr release method. It demonstrates that the new marker provides an alternative to currently used radioactive markers for the assessment of in vitro cellular cytotoxicity.展开更多
文摘Rats(Sprague Dawley,200-300g)wereoperated under anesthesia with nembuta1.Afterthe laminectomy,20-25%aqueous solution ofHRP(Sigma 6)was injected unilatera11y intothe spinal cord at cervical or lumbar leve1.Theanimals were allowed to survive for 36-48hours and transcardically perfused with 0.9%NaCl,3.5%paraforma1dehyde,and 10%sucr-ose in 0.1mol/L PB.The brain was removed andimmersed in 10%sucrose at 4℃.
文摘Ju Gong reported in 1981 that HRP could be anterogradely transported for a long distance by neurons. Based on this fact the rate of anterograde transport was investigated and at the same time the rate of retrograde transport and the time of disappearance of HRP in the labeled sites were observed. Twenty-two rabbits weighing 2—2.2 kg were used, HRP (30%, 1.2μ1) was injected unilaterally into the L6 spinal cord. The animals were decapitated 12 hr to 7 days after the injection and their brains were processed with o-dionisidine. Labeling in the brain stem was then searched for. The ante-
文摘In the hypothalamus there are many cells known to terminate in the posterior pituitary gland, which are mainly vasopressinergic and oxytocinergic neurons. Immunohistochemical studies have shown that they are concentrated in the supraoptic nucleus (SON) and the paraventricular nucleus (PVN) also found to be scattered in
文摘The centrifugal control of exteroceptors, particularly the efferent influence of sympathetic nerves on receptors has been well documented. Loewenstein and Chernetski observed that the activity of tactile receptors of the skin could be facilitated by sympathetic nerves. Santini et al. recently confirmed that the sympathetic nerves were distributed to the vicinity of sensory nerve endings, and the afferent
文摘With the progress of the horseradish peroxidase (HRP) technique, some new connections between the spinal cord and the brain have been discovered. After injecting HRP into the spinal cord of the cat, we have also found some connections hitherto undescribed or not fully described. The HRP is generally used as a retrograde tracer. Some short-distance HRP anterograde tracing studies have also been published.
文摘Most authors believed that the spinal cord was not directly connected with the hypothalamus. However, direct hypothalamo-spinal projection has been discovered recently in several species by means of the horseradish peroxidase tracing technique. No unequivoeal evidence of direct spino-hypothalamic projection is available. Seven rabbits were used in the present study. 30％ aqueous horseradish peroxidase (Sigma Ⅵ)
文摘Most of the reports on human placental villi endocrinology focus on the study of neuropeptides, pituitary-or gonadal-like hormones, growth factors and cytokines, and only a few of them concern the function of biogenic amine. We first discovered that trophoblast of placental villi can produce 5-hydroxytryptamine (5-HT) and that its
基金Project supported by the National Natural Science Foundation of China.
文摘A new time-resolved fluorimetric method for the measurement of natural killer (NK) cell cytotoxicity has been developed by labelling the target cell K562 with a new synthesized fluorescence marker KLUK. The method has advantages of higher sensitivity, time-saving, good reproducibility and has no radioactivity problems. A satisfactory result is obtained by comparing it with 51Cr release method. It demonstrates that the new marker provides an alternative to currently used radioactive markers for the assessment of in vitro cellular cytotoxicity.
