Background:The aim of this study was to investigate the influence of marking meth-ods on the outcomes of body composition analysis and provide guidance for the se-lection of marking methods in mouse body composition a...Background:The aim of this study was to investigate the influence of marking meth-ods on the outcomes of body composition analysis and provide guidance for the se-lection of marking methods in mouse body composition analysis.Methods:Male C57BL/6J mice aged 6 weeks were randomly assigned for pre-and post-ear tagging measurements.The body composition of the mice was measured using a small animal body composition analyzer,which provided measurements of the mass of fat,lean,and free fluid.Then,the mass of fat,lean and free fluid to body weight ratio was gained.Further data analysis was conducted to obtain the range and coeffi-cient of variation in body composition measurements for each mouse.The distribution of fat and lean tissue in the mice was also analyzed by comparing the fat-to-lean ratio.Results:(1)The mass of all body composition components in the ear tagging group was significantly lower than that in the control group.(2)There was a significant in-crease in the range and coefficient of variation of body composition measurements between the ear tagging group and the control group.(3)The fat-to-lean ratio in the ear tagging group was significantly lower than that in the control group.Conclusions:Ear tagging significantly lowered the results of body composition analy-sis in mice and higher the results of measurement error.Therefore,ear tagging should be avoided as much as possible when conducting body composition analysis experi-ments in mice.展开更多
Nonalcoholic fatty pancreas disease(NAFPD)is an emerging disease that has gained an increasing amount of attention in recent years.It describes fat accumulation in the pancreas with insignificant alcohol consumption,b...Nonalcoholic fatty pancreas disease(NAFPD)is an emerging disease that has gained an increasing amount of attention in recent years.It describes fat accumulation in the pancreas with insignificant alcohol consumption,but the pathogenesis is largely unknown.A wide range of terms have been used to describe the phenomenon of pancreatic fat accumulation,but NAFPD remains an under-recognized and non-independent disorder.Obesity,age,sex,race,and unhealthy lifestyle are established independent risk factors for NAFPD,which is strongly associated with metabolic syndrome,type 2 diabetes,pancreatitis,pancreatic fistula,pancreatic cancer,and nonalcoholic fatty liver disease.At present,imaging techniques are common diagnostic aids,but uniform criteria and consensus are lacking.Therapeutically,healthy diet,weight loss,and exercise are the mainstays to reduce pancreatic fat accumulation.It can be seen that there is a limited understanding of NAFPD at this stage and further exploration is needed.Previous studies have revealed that NAFPD may directly affect diagnosis and clinical decision-making.Therefore,exploring the pathophysiological mechanism and clinical associations of NAFPD is a major challenge for researchers and clinicians.展开更多
文摘Background:The aim of this study was to investigate the influence of marking meth-ods on the outcomes of body composition analysis and provide guidance for the se-lection of marking methods in mouse body composition analysis.Methods:Male C57BL/6J mice aged 6 weeks were randomly assigned for pre-and post-ear tagging measurements.The body composition of the mice was measured using a small animal body composition analyzer,which provided measurements of the mass of fat,lean,and free fluid.Then,the mass of fat,lean and free fluid to body weight ratio was gained.Further data analysis was conducted to obtain the range and coeffi-cient of variation in body composition measurements for each mouse.The distribution of fat and lean tissue in the mice was also analyzed by comparing the fat-to-lean ratio.Results:(1)The mass of all body composition components in the ear tagging group was significantly lower than that in the control group.(2)There was a significant in-crease in the range and coefficient of variation of body composition measurements between the ear tagging group and the control group.(3)The fat-to-lean ratio in the ear tagging group was significantly lower than that in the control group.Conclusions:Ear tagging significantly lowered the results of body composition analy-sis in mice and higher the results of measurement error.Therefore,ear tagging should be avoided as much as possible when conducting body composition analysis experi-ments in mice.
文摘Nonalcoholic fatty pancreas disease(NAFPD)is an emerging disease that has gained an increasing amount of attention in recent years.It describes fat accumulation in the pancreas with insignificant alcohol consumption,but the pathogenesis is largely unknown.A wide range of terms have been used to describe the phenomenon of pancreatic fat accumulation,but NAFPD remains an under-recognized and non-independent disorder.Obesity,age,sex,race,and unhealthy lifestyle are established independent risk factors for NAFPD,which is strongly associated with metabolic syndrome,type 2 diabetes,pancreatitis,pancreatic fistula,pancreatic cancer,and nonalcoholic fatty liver disease.At present,imaging techniques are common diagnostic aids,but uniform criteria and consensus are lacking.Therapeutically,healthy diet,weight loss,and exercise are the mainstays to reduce pancreatic fat accumulation.It can be seen that there is a limited understanding of NAFPD at this stage and further exploration is needed.Previous studies have revealed that NAFPD may directly affect diagnosis and clinical decision-making.Therefore,exploring the pathophysiological mechanism and clinical associations of NAFPD is a major challenge for researchers and clinicians.
