Halophilic alga Dunaliella bardawil (Chlorophyceae) was cultivated in artificialseawater (containing 3 .0, 1. 5 or 0. 5 mol/L of NaCl respectively) for at least two weeks; total RNAswere then extracted and 8 stable di...Halophilic alga Dunaliella bardawil (Chlorophyceae) was cultivated in artificialseawater (containing 3 .0, 1. 5 or 0. 5 mol/L of NaCl respectively) for at least two weeks; total RNAswere then extracted and 8 stable differential bands were harvested after DDRT-PCR and electrophoresis.The retrieved bands were amplified, subjected to electrophoresis again, and cloned into plasmid pUCm-Trespectively. After exelusion of false positive bands, We obtained a recombinant plasmid pTE containing afragment of cDNA, which was only specilically expressed under high salinity condition. Sequencing of展开更多
基金This work was supported by the Foundation of National Science and Technology Ministry,Grant No.J00-B-014,and Science and Technology Project of Xiamen City,Grant No.350222000104
文摘Halophilic alga Dunaliella bardawil (Chlorophyceae) was cultivated in artificialseawater (containing 3 .0, 1. 5 or 0. 5 mol/L of NaCl respectively) for at least two weeks; total RNAswere then extracted and 8 stable differential bands were harvested after DDRT-PCR and electrophoresis.The retrieved bands were amplified, subjected to electrophoresis again, and cloned into plasmid pUCm-Trespectively. After exelusion of false positive bands, We obtained a recombinant plasmid pTE containing afragment of cDNA, which was only specilically expressed under high salinity condition. Sequencing of
