Nuciferine alleviates collagen-induced arthritic in rats by inhibiting the proliferation and invasion of human arthritis-derived fibroblast-like synoviocytes and rectifying Th17/Treg imbalance

Rheumatoid arthritis(RA)is a chronic autoimmune disorder marked by persistent synovial inflammation and joint degradation,posing challenges in the development of effective treatments.Nuciferine,an alkaloid found in lotus leaf,has shown promising anti-inflammatory and anti-tumor effects,yet its efficacy in RA treatment remains unexplored.This study investigated the antiproliferative effects of nuciferine on the MH7A cell line,a human RA-derived fibroblast-like synoviocyte,revealing its ability to inhibit cell proliferation,promote apoptosis,induce apoptosis,and cause G1/S phase arrest.Additionally,nuciferine significantly reduced the migration and invasion capabilities of MH7A cells.The therapeutic potential of nuciferine was further evaluated in a collagen-induced arthritis(CIA)rat model,where it markedly alleviated joint swelling,synovial hyperplasia,cartilage injury,and inflammatory infiltration.Nuciferine also improved collagen-induced bone erosion,decreased pro-inflammatory cytokines and serum immunoglobulins(IgG,IgG1,IgG2a),and restored the balance between T helper(Th)17 and regulatory T cells in the spleen of CIA rats.These results indicate that nuciferine may offer therapeutic advantages for RA by decreasing the proliferation and invasiveness of FLS cells and correcting the Th17/Treg cell imbalance in CIA rats.

Targeted bile acids metabolomics in cholesterol gallbladder polyps and gallstones: From analytical method development towards application to clinical samples

Bile acids(BAs)are synthesized by the liver from cholesterol through several complementary pathways and aberrant cholesterol metabolism plays pivotal roles in the pathogeneses of cholesterol gallbladder polyps(CGP)and cholesterol gallstones(CGS).To date,there is neither systematic study on BAs profile of CGP or CGS,nor the relationship between them.To explore the metabolomics profile of plasma BAs in healthy volunteers,CGP and CGS patients,an ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)method was developed and validated for simultaneous determination of 42 free and conjugated BAs in human plasma.The developed method was sensitive and reproducible to be applied for the quantification of BAs in the investigation of plasma samples.The results show that,compared to healthy volunteers,CGP and CGS were both characterized by the significant decrease in plasma BAs pool size,furthermore CGP and CGS shared aberrant BAs metabolic characteristics.Chenodeoxycholic acid,glycochenodeoxycholic acid,l-muricholic acid,deoxycholic acid,and 7-ketolithocholic acid were shared potential markers of these two cholesterol gallbladder diseases.Subsequent analysis showed that clinical characteristics including cysteine,ornithine and body mass index might be closely related to metabolisms of certain BA modules.This work provides metabolomic information for the study of gallbladder diseases and analytical methodologies for clinical target analysis and efficacy evaluation related to BAs in medical institutions.

Advances on hormone-like activity of Panax ginseng and ginsenosides

Traditional Chinese medicine(TCM)has been paid much attentions due to the prevention and treatment of steroid hormone disorders.Ginseng,the root of Panax ginseng C.A.Meyer(Araliaceae),is one of the most valuable herbs in complementary and alternative medicines around the world.A series of dammarane triterpenoid saponins,also known as phytosteroids,were reported as the primary ingredients of Ginseng,and indicated broad spectral pharmacological actions,including anti-cancer,anti-inflammation and anti-fatigue.The skeletons of the dammarane triterpenoid aglycone are structurally similar to the steroid hormones.Both in vitro and in vivo studies showed that Ginseng and its active ingredients have beneficial hormone-like role in hormonal disorders.This review thus summarizes the structural similarities between hormones and dammarane ginsenosides and integrates the analogous effect of Ginseng and ginsenosides on prevention and treatment of hormonal disorders published in recent twenty years(1998–2018).The review may provide convenience for anticipate structure-function relationship between saponins structure and hormone-like effect.

Two new chromones and a new flavone glycoside from Imperata cylindrica

试图学习 Imperata cylindrica 的根茎的化学成分。混合物被硅石胶化和 Sephadex LH-20 列层析孤立的方法。他们的结构主要被分光镜的分析包括红外， HR-ESI-MS， 1D-NMR 和 2D-NMR 阐明。结果二新 chromones ，8-hydroxy-2-( 2-phenylethyl ) chromone ( 1 )和2-( 2-phenylethyl ) chromone-8-O -- D-glucopyranoside ( 2 )，和新黄酮 glycoside ， 4-methoxyflavone-6-O--D-glucopyranoside ( 5 )与五已知的混合物一起， flidersiachromone ( 3 )，5-hydroxy-2-( 2-phenylethyl ) chromone ( 4 )，黄酮( 6 )， 4-hydroxy-5-methoxyflavone ( 7 )和 5-hydroxyflavone ( 8 )从 Imperata cylindrica 被孤立。结论混合物 1， 2 和 5 是新混合物。