Excessive mercury ions(Hg^(2+)) in the environment can accumulate in human body along with the food chain to cause serious physiological reactions.The fluorescence probes were considered as convenient tool with great ...Excessive mercury ions(Hg^(2+)) in the environment can accumulate in human body along with the food chain to cause serious physiological reactions.The fluorescence probes were considered as convenient tool with great potential for Hg^(2+) detection.Most existing probes suffer from aggregation-induced quenching(ACQ) effects and insufficient sensitivity.Herein,a novel type of fluorophore was developed by combining the aggregation-induced emission(AIE) and excited state intramolecular proton transfer(ESIPT) characteristics.Subsequently,a phenyl thioformate group with photoinduced electron transfer(PET)effect was connected to give an efficient "turn-on" probe(HTM),which exhibited good selectivity toward Hg^(2+),short response time(30 min),coupled with extremely low detection limit(LOD=1.68 nmol/L).In addition,HTM was used successfully in real samples,cells and drug evaluation,underlying the superiority of HTM to detect Hg^(2+) in practical applications.展开更多
Intracellular pH is a key parameter related to various biological and pathological processes.In this study,a ratiometric pH fluorescent sensor ABTT was developed harnessing the amino-type excited-state intramolecular ...Intracellular pH is a key parameter related to various biological and pathological processes.In this study,a ratiometric pH fluorescent sensor ABTT was developed harnessing the amino-type excited-state intramolecular proton transfer(ESIPT) process.Relying on whether the ESIPT proceeds normally or not,ABTT exhibited the yellow fluorescence in acidic media,or cyan fluorescence in basic condition.According to the variation,ABTT behaved as a promising sensor which possessed fast and reversible response to pH change without interference from the biological substances,and exported a steady ratiometric signal(I_(478)/I_(546)).Moreover,due to the ESIPT effect,large Stokes shift and high quantum yield were also exhibited in ABTT.Furthermore,ABTT was applied for monitoring the pH changes in living cells and visualizing the pH fluctuations under oxidative stress successfully.These results elucidated great potential of ABTT in understanding pH-dependent physiological and pathological processes.展开更多
基金financial supports from the National Natural Science Foundation of China (Nos. 81971678 and 81671756)the Innovation Fund for Post graduate Students of Central South University (No. 2020zzts827)。
文摘Excessive mercury ions(Hg^(2+)) in the environment can accumulate in human body along with the food chain to cause serious physiological reactions.The fluorescence probes were considered as convenient tool with great potential for Hg^(2+) detection.Most existing probes suffer from aggregation-induced quenching(ACQ) effects and insufficient sensitivity.Herein,a novel type of fluorophore was developed by combining the aggregation-induced emission(AIE) and excited state intramolecular proton transfer(ESIPT) characteristics.Subsequently,a phenyl thioformate group with photoinduced electron transfer(PET)effect was connected to give an efficient "turn-on" probe(HTM),which exhibited good selectivity toward Hg^(2+),short response time(30 min),coupled with extremely low detection limit(LOD=1.68 nmol/L).In addition,HTM was used successfully in real samples,cells and drug evaluation,underlying the superiority of HTM to detect Hg^(2+) in practical applications.
基金the financial supports from National Natural Science Foundation of China (Nos.81971678 and 81671756)the Innovation Fund for Postgraduate Students of Central South University (No.2019zzts1019)。
文摘Intracellular pH is a key parameter related to various biological and pathological processes.In this study,a ratiometric pH fluorescent sensor ABTT was developed harnessing the amino-type excited-state intramolecular proton transfer(ESIPT) process.Relying on whether the ESIPT proceeds normally or not,ABTT exhibited the yellow fluorescence in acidic media,or cyan fluorescence in basic condition.According to the variation,ABTT behaved as a promising sensor which possessed fast and reversible response to pH change without interference from the biological substances,and exported a steady ratiometric signal(I_(478)/I_(546)).Moreover,due to the ESIPT effect,large Stokes shift and high quantum yield were also exhibited in ABTT.Furthermore,ABTT was applied for monitoring the pH changes in living cells and visualizing the pH fluctuations under oxidative stress successfully.These results elucidated great potential of ABTT in understanding pH-dependent physiological and pathological processes.
