The aim of thisstudy was to conduct a randomized, double-blind and placebo-controlled study to investigate the effects of D-004, a lipid extract of the Roystonea regia fruit that prevents testosterone- and phenylepine...The aim of thisstudy was to conduct a randomized, double-blind and placebo-controlled study to investigate the effects of D-004, a lipid extract of the Roystonea regia fruit that prevents testosterone- and phenylepinephrine- induced prostate hyperplasia in rodents, on plasma oxidative markers in healthy men. We enrolled male volunteers （20-55 years） in good health and without lower urinary tract symptoms. Thirty-four eligible participants were randomized to placebo or D-004 （320 mg） capsules administered daily for 6 weeks. An interim check-up and a final visit were conducted after 3 and 6 weeks of therapy, respectively. Physical examinations were performed at each visit, and laboratory tests were performed at baseline and at treatment completion. Oxidative variables included plasma malondialdehyde （MDA）, total hydroxyperoxides （TOH）, sulphydryl （SH） groups and total antioxidant status （TAS）. We assessed treatment compliance and addressed adverse experiences （AEs） at weeks 3 and 6. At week 6, with D-004, the mean reductions of plasma MDA （26.7%）, TOH （18.8%） and SH groups （31.6%）, and the mean increase of TAS （35.3%） were significantly different from those of placebo （P 〈 0.001 for plasma TAS, P 〈 0.0001 for all other comparisons）. D-004 did not differ from the placebo in safety indicators. There were two withdrawals （both in the D-004 group）, with one due to dyspepsia （the only AE during the trial）. In conclusion, D-004 displayed antioxidant effects on plasma oxidative markers in healthy men, which was consistent with findings from laboratory experimental studies.展开更多
Deep body temperature of 57 male Wistar rats was measured in response to systemic (i.v. or i.p.) injection of Escherichia coli lipopolysaccharide (LPS). Animals were preliminarily (4 weeks before the experiment) treat...Deep body temperature of 57 male Wistar rats was measured in response to systemic (i.v. or i.p.) injection of Escherichia coli lipopolysaccharide (LPS). Animals were preliminarily (4 weeks before the experiment) treated with microinjections of the toxic dose of glutamate receptors agonists or vehicle into solitary tract nucleus or caudal part of ventrolateral medulla. Microinjections of the glutamate receptors agonists provoked a local destruction of brain tissue, which was accompanied by the significant transformation (amplification or attenuation) of temperature response to the systemic injection of 3 μg/kg E. coli LPS. Animals treated with vehicle did not demonstrate significant changes in their response to LPS. Obtained data testify that impairment of central nervous structure involved in the regulation and maintenance of deep body temperature is accompanied by atypical development of E. coli LPS-induced fever.展开更多
文摘The aim of thisstudy was to conduct a randomized, double-blind and placebo-controlled study to investigate the effects of D-004, a lipid extract of the Roystonea regia fruit that prevents testosterone- and phenylepinephrine- induced prostate hyperplasia in rodents, on plasma oxidative markers in healthy men. We enrolled male volunteers （20-55 years） in good health and without lower urinary tract symptoms. Thirty-four eligible participants were randomized to placebo or D-004 （320 mg） capsules administered daily for 6 weeks. An interim check-up and a final visit were conducted after 3 and 6 weeks of therapy, respectively. Physical examinations were performed at each visit, and laboratory tests were performed at baseline and at treatment completion. Oxidative variables included plasma malondialdehyde （MDA）, total hydroxyperoxides （TOH）, sulphydryl （SH） groups and total antioxidant status （TAS）. We assessed treatment compliance and addressed adverse experiences （AEs） at weeks 3 and 6. At week 6, with D-004, the mean reductions of plasma MDA （26.7%）, TOH （18.8%） and SH groups （31.6%）, and the mean increase of TAS （35.3%） were significantly different from those of placebo （P 〈 0.001 for plasma TAS, P 〈 0.0001 for all other comparisons）. D-004 did not differ from the placebo in safety indicators. There were two withdrawals （both in the D-004 group）, with one due to dyspepsia （the only AE during the trial）. In conclusion, D-004 displayed antioxidant effects on plasma oxidative markers in healthy men, which was consistent with findings from laboratory experimental studies.
文摘Deep body temperature of 57 male Wistar rats was measured in response to systemic (i.v. or i.p.) injection of Escherichia coli lipopolysaccharide (LPS). Animals were preliminarily (4 weeks before the experiment) treated with microinjections of the toxic dose of glutamate receptors agonists or vehicle into solitary tract nucleus or caudal part of ventrolateral medulla. Microinjections of the glutamate receptors agonists provoked a local destruction of brain tissue, which was accompanied by the significant transformation (amplification or attenuation) of temperature response to the systemic injection of 3 μg/kg E. coli LPS. Animals treated with vehicle did not demonstrate significant changes in their response to LPS. Obtained data testify that impairment of central nervous structure involved in the regulation and maintenance of deep body temperature is accompanied by atypical development of E. coli LPS-induced fever.
