Objective： Ultraviolet （UV） radiation is one of the important cataract risk factors, However, the pathogenesis is still poorly understood. The migration of human lens epithelial cells（HLECs） plays a Clucial role ...Objective： Ultraviolet （UV） radiation is one of the important cataract risk factors, However, the pathogenesis is still poorly understood. The migration of human lens epithelial cells（HLECs） plays a Clucial role in the remodeling of lens capsule and cataract formation. The purpose of this study is to investigate the mechanism of UV inducing cataractogenesis. Methods：The toxicity of UV-irradiation on HLECs was assessed by Methyl thiazolyl tetrazolium（MTT） assay. The activity of matrix metalloproteinase-2（MMP-2） was observed by Gelatin zymography. The migration of HLECs was examined by Cell Track Motility. Results：UV-irradiation does great harm to HLECs, and may induce apoptosis in the cells when UV higher than 15 mj/cm2. UV significantly increased MMP-2 activity in a timedependent manner. In addition, the irradiation could induce the migration of HLECs. Conclusion：UV-irradiation could induce the migration of HLECs by increasing the activity of MMP-2.展开更多
基金This work was supported by the project of the Natural Science Foun- dation of Jiangsu province(No.BK2007234) the Institution Natural Science Foundation of Jiangsu province(No.07KJD320140)
文摘Objective： Ultraviolet （UV） radiation is one of the important cataract risk factors, However, the pathogenesis is still poorly understood. The migration of human lens epithelial cells（HLECs） plays a Clucial role in the remodeling of lens capsule and cataract formation. The purpose of this study is to investigate the mechanism of UV inducing cataractogenesis. Methods：The toxicity of UV-irradiation on HLECs was assessed by Methyl thiazolyl tetrazolium（MTT） assay. The activity of matrix metalloproteinase-2（MMP-2） was observed by Gelatin zymography. The migration of HLECs was examined by Cell Track Motility. Results：UV-irradiation does great harm to HLECs, and may induce apoptosis in the cells when UV higher than 15 mj/cm2. UV significantly increased MMP-2 activity in a timedependent manner. In addition, the irradiation could induce the migration of HLECs. Conclusion：UV-irradiation could induce the migration of HLECs by increasing the activity of MMP-2.
