A novel synthesis of the functional carbohydrate 2-amino-2-deoxy-D-gluconic acid was introduced and its complex formation with Cu（Ⅱ） was investigated to obtain the stability constant for its further applications to...A novel synthesis of the functional carbohydrate 2-amino-2-deoxy-D-gluconic acid was introduced and its complex formation with Cu（Ⅱ） was investigated to obtain the stability constant for its further applications to the food and pharmaceutical industries. The equilibrium was investigated by spectrophotometric measurements and processed by dual-series linear regression method. Results： the yield of 2-amino-2-deoxy-D-gluconic acid is 70%. The complexation molar ratio is 1 ： 2, the molar apsorptivity of the complex is 39.906 L·mol^-1·cm^-1 at 630 nm, and the stability constant βn is 6.24 × 10^5.展开更多
Alizarin red （AR） can bind with aloe polysaccharide （APS） in doubly de-ionized water to form a red complex resulting in fluorescence quenching of it. The maximum fluorescence quenching wavelength is 572 nm. The ch...Alizarin red （AR） can bind with aloe polysaccharide （APS） in doubly de-ionized water to form a red complex resulting in fluorescence quenching of it. The maximum fluorescence quenching wavelength is 572 nm. The chromogenic reaction is rapid and the fluorescence intensity remains stable for at least 2 h at room temperature. The quenched fluorescence intensity （AF） is directly proportional to APS concentration. Based on this interaction, a sensitive and selective fluorophotometric method is proposed for the determination of APS. The optimal experiment conditions were established. The corresponding linear equation is AF=0.8807C＋1.8132, R2=0.9999. The quantification and detection limits are 0.4752 and 0.1425 ggomL l, respectively. The linear range is 0.444--16.65 μg·mL^-1 for APS and the mean recovery （100.2±2）%, RSD≤3%. The effect of various substances on the determination of APS was also investigated in detail, and the results show that most of the studied coexistent substances can be tolerated in considerable amounts. The proposed method is sensitive, simple, fast and suitable for routine assay.展开更多
基金Project supported by the Youth Science Foundation of Southern Yangtze University （No. 0004416）.
文摘A novel synthesis of the functional carbohydrate 2-amino-2-deoxy-D-gluconic acid was introduced and its complex formation with Cu（Ⅱ） was investigated to obtain the stability constant for its further applications to the food and pharmaceutical industries. The equilibrium was investigated by spectrophotometric measurements and processed by dual-series linear regression method. Results： the yield of 2-amino-2-deoxy-D-gluconic acid is 70%. The complexation molar ratio is 1 ： 2, the molar apsorptivity of the complex is 39.906 L·mol^-1·cm^-1 at 630 nm, and the stability constant βn is 6.24 × 10^5.
基金Project supported by Object-oriented Project of State Key Laboratory of Food Science and Technology （No. SKLF-MB-200805） and Youth Science Foundation of Jiangnan University （No. 104000-52210688）.
文摘Alizarin red （AR） can bind with aloe polysaccharide （APS） in doubly de-ionized water to form a red complex resulting in fluorescence quenching of it. The maximum fluorescence quenching wavelength is 572 nm. The chromogenic reaction is rapid and the fluorescence intensity remains stable for at least 2 h at room temperature. The quenched fluorescence intensity （AF） is directly proportional to APS concentration. Based on this interaction, a sensitive and selective fluorophotometric method is proposed for the determination of APS. The optimal experiment conditions were established. The corresponding linear equation is AF=0.8807C＋1.8132, R2=0.9999. The quantification and detection limits are 0.4752 and 0.1425 ggomL l, respectively. The linear range is 0.444--16.65 μg·mL^-1 for APS and the mean recovery （100.2±2）%, RSD≤3%. The effect of various substances on the determination of APS was also investigated in detail, and the results show that most of the studied coexistent substances can be tolerated in considerable amounts. The proposed method is sensitive, simple, fast and suitable for routine assay.
