To study the mechanisms of wrist-ankle acupuncture for prevention and treatment of diabeticperipheral neuritis.Methods:Ninety cases of diabetic peripheral neuritis were randomly divided into 3groups,and treated respec...To study the mechanisms of wrist-ankle acupuncture for prevention and treatment of diabeticperipheral neuritis.Methods:Ninety cases of diabetic peripheral neuritis were randomly divided into 3groups,and treated respectively with wrist-ankle acupuncture,body-acupuncture,and the western routinemedical treatment,with 30 cases in each of the groups;and therapeutic effects and laboratory resultscompared.Results:It is proved that the therapeutic effects of the wrist-ankle acupuncture group and bodyacupuncture group were significantly superior to those of the control group,with no significant differencesbetween the former two groups.Conclusion:Wrist-ankle acupuncture has the actions of improving themetabolisms of blood sugar and blood-lipid,lowering down blood viscosity,and restoring the functions ofperipheral nerve cells,thus giving definite therapeutic effects for diabetic peripheral neuritis.展开更多
The reinforcing and reducing are the two major methods used in the acupuncture-moxibustion treatment. Based on Internal Classic, Elementary Medicine and Great Compendium of Acupuncture and Moxibustion, this essay intr...The reinforcing and reducing are the two major methods used in the acupuncture-moxibustion treatment. Based on Internal Classic, Elementary Medicine and Great Compendium of Acupuncture and Moxibustion, this essay introduces the various manipulation techniques of reinforcing and reducing in acupuncture-moxibustion treatment, and the indicated acupoints. Clinically, according to the specific conditions of the patient, specific methods and points should be adopted and selected in the treatment of various kinds of diseases.展开更多
[ Objectives] This study was conducted to evaluate the acute oral toxicity of Shenrong Bunao Capsule, and to provide a theoretical basis for drug devel- opmcn! and clinical trims. [ Methods] Kunming mice were randomly...[ Objectives] This study was conducted to evaluate the acute oral toxicity of Shenrong Bunao Capsule, and to provide a theoretical basis for drug devel- opmcn! and clinical trims. [ Methods] Kunming mice were randomly assigned into two groups: an experimental group and a control group. In experimental group, Shenrong Bunao Capsule solution at maximum concentration of 45% was administered orally by gavage once at a dose of 30 ml/kg body weight ( BW), while an equal volume of saline solution was given in the control group. Then, the appearance, behavior, mental state, diet, feces, urine, coat, skin color, respiration, nose, eyes and oral secretions and other daily activities of the mice were observed for 14 d after drug administration. At the end of the experiment, the mice were dissected, and their main organs were examined histopathologically. [Results] The appearance, behavior, respiration, body posture, response to stimuli and weight gain of the mice in control group were all normal. All the mice given 13. 500 g/kg BW Shenrong Bunao Capsule were alive 14 d after drug administration, and their appearance, behavior, respiration, body posture, response to stimuli and weight gains did not show any abnormality. In addition, the body weights of male and female mice had no obvious difference between the experimental group and the control group at the binging, 7 and 14 d after drug administration (P 〈0.05). [ Conclusions] A maximum dose 13. 500 g/kg BW Shenrong Bunao Capsule, which was equivalent to 141 times of the recommended dosage for adult men was given to the mice, and did not result in any apparent toxic effects, suggesting that the Chines herbal preparation Shenrong Bunao Capsule has very little acute toxicity, and the commonly recommended dosage in clinical trials is safe.展开更多
With quercetin content and yield of dry extract as investigation indices, three factors, amount of water added, decoction time and decoction times were investigated by an orthogonal test, so as to select the optimal w...With quercetin content and yield of dry extract as investigation indices, three factors, amount of water added, decoction time and decoction times were investigated by an orthogonal test, so as to select the optimal water extraction process for Zihua Qinghuo Capsule. The determined extraction process was adding 8 times of water in five herbs including Viola philippica and decocting for 2 times, 1.5 h each time. Pilot scale test demonstrated that the selected process is stable and feasible, and could provide reliable reference for practical production.展开更多
[Objectives] An acute toxicity test of Miantan Fuzheng Powder was conducted on rabbits,which were given Miantan Fuzheng Powder at its maximum administration dosage and observed for 14 d,so as to provide reference for ...[Objectives] An acute toxicity test of Miantan Fuzheng Powder was conducted on rabbits,which were given Miantan Fuzheng Powder at its maximum administration dosage and observed for 14 d,so as to provide reference for medicine development and clinical test.[Methods] New zealand rabbits were divided into four groups randomly,each of which included 4 rabbits,half male and half female.The Miantan Fuzheng Powder-intact skin and Miantan Fuzheng Powderdamaged skin groups were applied with Miantan Fuzheng Powder once at a dose of 5 g/kg body weight,and the blank control group-intact skin and blank controldamaged skin groups were given the auxiliary material( vaseline) once.The rabbits were observed continuously for 14 d after the administration,and variation in body weight was recorded during the observation and then statistically analyzed with SPSS.After the 14 d of observation,the animals were killed through pentobarbital anesthesia and anatomized to observe main visceral organs including heart,liver,spleen lung and kidney.If toxic effect or organ abnormality exists,pathological sections would be prepared and observed.[Results] Rabbits in the blank control and Miantan Fuzheng Powder groups all showed normal appearance,behavior,breath state,position and posture,response to stimulation and growth of body weight,and no irritative erythema and edema on the skin,and the damaged skin recovered naturally within 14 d.No obvious abnormality was observed on visceral organs in autopsy.The Miantan Fuzheng Powder-intact skin and Miantan Fuzheng Powder-damaged skin groups had no significant differences in body weight from the blank control group-intact skin and blank control-damaged skin groups before administration,and on the 7^(th) and 14^(th) day after administration.[Conclusions] In this acute toxicity test,the Miantan Fuzheng Powder-intact skin and Miantan Fuzheng Powder-damaged skin groups were given the drug at the maximum administration dosage of 5 g/kg body weight,which was about 119 times of the clinical dose for human,and no obvious toxic effect was observed.展开更多
[ Objectives] This study was conducted to establish a method for microbial limit test of Tongmai Tangyanming Capsule. [ Methods] According to the Chinese Pharmacopoei 2015 edition of the four general rules, the microb...[ Objectives] This study was conducted to establish a method for microbial limit test of Tongmai Tangyanming Capsule. [ Methods] According to the Chinese Pharmacopoei 2015 edition of the four general rules, the microbiological limits of non-sterile products were verified. [ Results] Tongmai Tangyanming Capsule has a strong inhibitory effect on Bacillus subtilis and Candida albicans. The antimicrobial activity was significantly eliminated after increasing the diluent ( 1 : 20) ; and the recoveries were in the range of 0.5 -2 when the total quantities of aerobic microbes were determined by dilution method (1:20). When the total quantities of mould and yeast were determined by dilution method (1:20), the recoveries were from 0.5 to 2, and Escherichia coli, cholate-tolerant Gram-negative bacteria and Salmonella can be detected by the test solution dilution method. [ Condusions] With the plate method, the total quantities of aerobic bacteria and the quantities of mould and yeast were examined by increasing diluent, and conventional method was used for examination of control bacteria including E. coli, cholatetolerant Gram-negative bacteria and Salmonella. This method has been proved effective for microbial limit test of Tongmai Tangyanming Capsule, and can effectively control the quality of the preparation. The method is accurate and reliable.展开更多
[Objectives] This study was conducted to establish a method for microbial limit test of Compound Gangbangui Capsule. [Methods] According to the Chinese Pharmacopoei 2015 edition of the four general rules,the microbiol...[Objectives] This study was conducted to establish a method for microbial limit test of Compound Gangbangui Capsule. [Methods] According to the Chinese Pharmacopoei 2015 edition of the four general rules,the microbiological limits of non-sterile products were verified. [Results]Compound Gangbangui Capsule has a strong inhibitory effect on S. aureus,and the antimicrobial activity was significantly eliminated after increasing the diluent( 1∶ 20); and the recoveries were in the range of 0. 5-2. 0 when the total quantities of aerobic microbes were determined by dilution method( 1∶ 20). When the total quantities of mould and yeast were determined by conventional method( 1∶ 20),the recoveries were from 0. 5 to 2. 0,and Escherichia coli can be detected in experimental groups by the test solution dilution method. [Conclusions]With the plate method,the total quantities of aerobic bacteria and the quantities of mould and yeast were examined by increasing diluent and conventional method,respectively,and control bacteria,E. coli was examined by conventional method. This method has been proved effective for microbial limit test of Compound Gangbangui Capsule,and can effectively control the quality of the preparation. The method is accurate and reliable.展开更多
Electroacupuncture (EA) at Zhongliao (BL33) can improve the symptoms of overactive bladder (OAB), such as urinary frequency, urgency, and incontinence. However, its performance compared with other acupoints rema...Electroacupuncture (EA) at Zhongliao (BL33) can improve the symptoms of overactive bladder (OAB), such as urinary frequency, urgency, and incontinence. However, its performance compared with other acupoints remains unclear. This study investigated the effects of EA at BL33 with deep needling on rats with OAB by detecting urodynamics in eight groups: no intervention group, D-BL33 group (deep needling at BL33), S-BL33 group (shallow needling at BL33), non-acupoint group (needling at the non-acupoint next to BL33), Weizhong (BL40) group, Sanyinjiao (SP6) group, Tongtian (BL7) group, and Hegu (LI4) group. Results revealed that EA at BL33 with deep needling, BL40, and SP6 prolonged the intercontraction interval (ICI) of rats with OAB (P= 0.001, P = 0.005, P = 0.046, respectively, post-treatment vs. post-modeling). Furthermore, the change in ICI from post-modeling in the D-BL33 group was significantly greater than those of the no intervention and other EA groups (all P 〈 0.01). Significantly shortened vesical micturition time (VMT) and elevated maximum detrusor pressure (MDP) were also observed in the D-BL33 group (P = 0.017 and P = 0.024, respectively, post-treatment vs. post-modeling). However, no statistically significant differences in the changes of VMT and MDP from post- modeling were observed between D-BL33 and the other EA groups. In conclusion, EA at BL33 with deep needling may inhibit acetic-acid-induced OAB more effectively.展开更多
文摘To study the mechanisms of wrist-ankle acupuncture for prevention and treatment of diabeticperipheral neuritis.Methods:Ninety cases of diabetic peripheral neuritis were randomly divided into 3groups,and treated respectively with wrist-ankle acupuncture,body-acupuncture,and the western routinemedical treatment,with 30 cases in each of the groups;and therapeutic effects and laboratory resultscompared.Results:It is proved that the therapeutic effects of the wrist-ankle acupuncture group and bodyacupuncture group were significantly superior to those of the control group,with no significant differencesbetween the former two groups.Conclusion:Wrist-ankle acupuncture has the actions of improving themetabolisms of blood sugar and blood-lipid,lowering down blood viscosity,and restoring the functions ofperipheral nerve cells,thus giving definite therapeutic effects for diabetic peripheral neuritis.
文摘The reinforcing and reducing are the two major methods used in the acupuncture-moxibustion treatment. Based on Internal Classic, Elementary Medicine and Great Compendium of Acupuncture and Moxibustion, this essay introduces the various manipulation techniques of reinforcing and reducing in acupuncture-moxibustion treatment, and the indicated acupoints. Clinically, according to the specific conditions of the patient, specific methods and points should be adopted and selected in the treatment of various kinds of diseases.
基金Supported by the Science and Technology Development Project of Guizhou Province(SY[2015]3028-3)
文摘[ Objectives] This study was conducted to evaluate the acute oral toxicity of Shenrong Bunao Capsule, and to provide a theoretical basis for drug devel- opmcn! and clinical trims. [ Methods] Kunming mice were randomly assigned into two groups: an experimental group and a control group. In experimental group, Shenrong Bunao Capsule solution at maximum concentration of 45% was administered orally by gavage once at a dose of 30 ml/kg body weight ( BW), while an equal volume of saline solution was given in the control group. Then, the appearance, behavior, mental state, diet, feces, urine, coat, skin color, respiration, nose, eyes and oral secretions and other daily activities of the mice were observed for 14 d after drug administration. At the end of the experiment, the mice were dissected, and their main organs were examined histopathologically. [Results] The appearance, behavior, respiration, body posture, response to stimuli and weight gain of the mice in control group were all normal. All the mice given 13. 500 g/kg BW Shenrong Bunao Capsule were alive 14 d after drug administration, and their appearance, behavior, respiration, body posture, response to stimuli and weight gains did not show any abnormality. In addition, the body weights of male and female mice had no obvious difference between the experimental group and the control group at the binging, 7 and 14 d after drug administration (P 〈0.05). [ Conclusions] A maximum dose 13. 500 g/kg BW Shenrong Bunao Capsule, which was equivalent to 141 times of the recommended dosage for adult men was given to the mice, and did not result in any apparent toxic effects, suggesting that the Chines herbal preparation Shenrong Bunao Capsule has very little acute toxicity, and the commonly recommended dosage in clinical trials is safe.
基金Supported by Guiyang Science and Technology Planning Project(ZKHT[2017-4]21)
文摘With quercetin content and yield of dry extract as investigation indices, three factors, amount of water added, decoction time and decoction times were investigated by an orthogonal test, so as to select the optimal water extraction process for Zihua Qinghuo Capsule. The determined extraction process was adding 8 times of water in five herbs including Viola philippica and decocting for 2 times, 1.5 h each time. Pilot scale test demonstrated that the selected process is stable and feasible, and could provide reliable reference for practical production.
基金Supported by Guizhou Scientific and Technological Cooperation Program[QKHLHZ(2015)7254]
文摘[Objectives] An acute toxicity test of Miantan Fuzheng Powder was conducted on rabbits,which were given Miantan Fuzheng Powder at its maximum administration dosage and observed for 14 d,so as to provide reference for medicine development and clinical test.[Methods] New zealand rabbits were divided into four groups randomly,each of which included 4 rabbits,half male and half female.The Miantan Fuzheng Powder-intact skin and Miantan Fuzheng Powderdamaged skin groups were applied with Miantan Fuzheng Powder once at a dose of 5 g/kg body weight,and the blank control group-intact skin and blank controldamaged skin groups were given the auxiliary material( vaseline) once.The rabbits were observed continuously for 14 d after the administration,and variation in body weight was recorded during the observation and then statistically analyzed with SPSS.After the 14 d of observation,the animals were killed through pentobarbital anesthesia and anatomized to observe main visceral organs including heart,liver,spleen lung and kidney.If toxic effect or organ abnormality exists,pathological sections would be prepared and observed.[Results] Rabbits in the blank control and Miantan Fuzheng Powder groups all showed normal appearance,behavior,breath state,position and posture,response to stimulation and growth of body weight,and no irritative erythema and edema on the skin,and the damaged skin recovered naturally within 14 d.No obvious abnormality was observed on visceral organs in autopsy.The Miantan Fuzheng Powder-intact skin and Miantan Fuzheng Powder-damaged skin groups had no significant differences in body weight from the blank control group-intact skin and blank control-damaged skin groups before administration,and on the 7^(th) and 14^(th) day after administration.[Conclusions] In this acute toxicity test,the Miantan Fuzheng Powder-intact skin and Miantan Fuzheng Powder-damaged skin groups were given the drug at the maximum administration dosage of 5 g/kg body weight,which was about 119 times of the clinical dose for human,and no obvious toxic effect was observed.
基金Supported by Special Fund for Research and Development of Traditional Chinese Medicine Modern Science and Technology Industry of Guizhou Province(qkhzyz[2011]5012)
文摘[ Objectives] This study was conducted to establish a method for microbial limit test of Tongmai Tangyanming Capsule. [ Methods] According to the Chinese Pharmacopoei 2015 edition of the four general rules, the microbiological limits of non-sterile products were verified. [ Results] Tongmai Tangyanming Capsule has a strong inhibitory effect on Bacillus subtilis and Candida albicans. The antimicrobial activity was significantly eliminated after increasing the diluent ( 1 : 20) ; and the recoveries were in the range of 0.5 -2 when the total quantities of aerobic microbes were determined by dilution method (1:20). When the total quantities of mould and yeast were determined by dilution method (1:20), the recoveries were from 0.5 to 2, and Escherichia coli, cholate-tolerant Gram-negative bacteria and Salmonella can be detected by the test solution dilution method. [ Condusions] With the plate method, the total quantities of aerobic bacteria and the quantities of mould and yeast were examined by increasing diluent, and conventional method was used for examination of control bacteria including E. coli, cholatetolerant Gram-negative bacteria and Salmonella. This method has been proved effective for microbial limit test of Tongmai Tangyanming Capsule, and can effectively control the quality of the preparation. The method is accurate and reliable.
基金Supported by Traditional Chinese Medicine and Ethnic Minority Medicine Technique Research Subject of Guizhou Administrative Bureau of TCM(QZYY2011-40)
文摘[Objectives] This study was conducted to establish a method for microbial limit test of Compound Gangbangui Capsule. [Methods] According to the Chinese Pharmacopoei 2015 edition of the four general rules,the microbiological limits of non-sterile products were verified. [Results]Compound Gangbangui Capsule has a strong inhibitory effect on S. aureus,and the antimicrobial activity was significantly eliminated after increasing the diluent( 1∶ 20); and the recoveries were in the range of 0. 5-2. 0 when the total quantities of aerobic microbes were determined by dilution method( 1∶ 20). When the total quantities of mould and yeast were determined by conventional method( 1∶ 20),the recoveries were from 0. 5 to 2. 0,and Escherichia coli can be detected in experimental groups by the test solution dilution method. [Conclusions]With the plate method,the total quantities of aerobic bacteria and the quantities of mould and yeast were examined by increasing diluent and conventional method,respectively,and control bacteria,E. coli was examined by conventional method. This method has been proved effective for microbial limit test of Compound Gangbangui Capsule,and can effectively control the quality of the preparation. The method is accurate and reliable.
基金This study was supported by the National Natural Science Foundation of China (Nos. 81173353 and 81373732).
文摘Electroacupuncture (EA) at Zhongliao (BL33) can improve the symptoms of overactive bladder (OAB), such as urinary frequency, urgency, and incontinence. However, its performance compared with other acupoints remains unclear. This study investigated the effects of EA at BL33 with deep needling on rats with OAB by detecting urodynamics in eight groups: no intervention group, D-BL33 group (deep needling at BL33), S-BL33 group (shallow needling at BL33), non-acupoint group (needling at the non-acupoint next to BL33), Weizhong (BL40) group, Sanyinjiao (SP6) group, Tongtian (BL7) group, and Hegu (LI4) group. Results revealed that EA at BL33 with deep needling, BL40, and SP6 prolonged the intercontraction interval (ICI) of rats with OAB (P= 0.001, P = 0.005, P = 0.046, respectively, post-treatment vs. post-modeling). Furthermore, the change in ICI from post-modeling in the D-BL33 group was significantly greater than those of the no intervention and other EA groups (all P 〈 0.01). Significantly shortened vesical micturition time (VMT) and elevated maximum detrusor pressure (MDP) were also observed in the D-BL33 group (P = 0.017 and P = 0.024, respectively, post-treatment vs. post-modeling). However, no statistically significant differences in the changes of VMT and MDP from post- modeling were observed between D-BL33 and the other EA groups. In conclusion, EA at BL33 with deep needling may inhibit acetic-acid-induced OAB more effectively.