Recently,a number of reports about pangolin have become hot news:The Chinese Pharmacopoeia(2020 edition)has not continued to include the drug-using standards for pangolin(Manis Squama),aristolochic(Aristolochia debili...Recently,a number of reports about pangolin have become hot news:The Chinese Pharmacopoeia(2020 edition)has not continued to include the drug-using standards for pangolin(Manis Squama),aristolochic(Aristolochia debilis Sieb.et Zucc),celestial vine(Fibraurea recisa Pierre),and Chinese patent drug Huanglian Yanggan pills(approval number by China State Food and Drug Administration:Z200113194).On June 5,2020,the China Forestry Administration Bureau and the Grassland Bureau co-issued an announcement to upgrade all the species of genus pangolins from the national second level of the protected wildlife to the first level.The new coronavirus(SARS-CoV-2)is also highly similar to the beta coronavirus isolated from pangolin[1].展开更多
Objective:Based on the network pharmacology approach and molecular docking technology,the core targets of dihydrotanshinone I(DHT)for the treatment of helicobacter pylori(Hp)infection were searched and the potential m...Objective:Based on the network pharmacology approach and molecular docking technology,the core targets of dihydrotanshinone I(DHT)for the treatment of helicobacter pylori(Hp)infection were searched and the potential mechanisms of drug therapy were explored.Methods:The TCMSPdatabase and Swiss Target Prediction database were employed to identify drug targets.To mine disease targets based on GeneCards,OMIM,DrugBank,DisGeNET,and TTDdatabases.Then the two were intersected to obtain common targets.The proteinproteininteraction(PPI)networkmap of common targets was constructed on the basis of the String network platform and Cytoscape software,and the targets with degree values over 1/2 maximum degree value were selected as core targets.Molecular docking verification of DHTand core targets were performed using AutoDock and PyMOL software.Finally,gene ontology(GO)functional enrichment analysis andKyoto Encyclopediaof Genes and Genomes(KEGG)pathway enrichment analysis of the common targets were carried out using the Metascape database and R-4.0.2-win software.Results:A total of 13 targets of DHTwas extracted for the treatment of Hp,and five core targets,includingSignal transducerand activator of transcription 1(STAT1),Signal transducerand activator of transcription 3(STAT3),Prostaglandin G synthase 2(PTGS2),Signal transducerand activator of transcription 4(STAT4)and Indoleamine 2,3-dioxygenase 1(IDO1),were screened according to their degree values.Molecular docking indicated that DHThad an excellent binding to the core target.29 pathways were yielded by KEGG enrichment analysis,and a total of 48 biological processes,7 cellular components and 13 molecular functions were derived from GO enrichment analysis.Conclusion:DHTmay decrease pro-inflammatory factor expression and immune cell infiltration to treat Hpinfection via the janus kinase(JAK)-signal transducer and activator of transcription(STAT)signaling pathway regulated by STAT1,STAT3,STAT4,etc.展开更多
Objective To explore the therapeutic effect of naringin on colorectal cancer(CRC)and the related mechanism.Methods Cell counting kit-8(CCK-8)assay and annexin V-FITC/PI assay were used to detect the effect of naringin...Objective To explore the therapeutic effect of naringin on colorectal cancer(CRC)and the related mechanism.Methods Cell counting kit-8(CCK-8)assay and annexin V-FITC/PI assay were used to detect the effect of naringin(50–400µg/mL)on cell proliferation and apoptosis of CRC cells,respectively.The scratch wound assay and transwell migration assay were used to assess the effect of naringin on CRC cell migration.Four-week-old male nude mice were injected with HCT116 cells subcutaneously to establish the tumor xenograft model.Naringin was injected intraperitoneally at 50 mg/(kg·d),with solvent and 5-fluorouracil treatment as control.The width and length of the tumors were measured and recorded every 6 days,and tumor tissues were photographed and weighed on the last day of the 24-d observation period.Immunohistochemical staining for caspase-3,proliferating cell nuclear antigen and TUNEL assay were used to evaluate the effect of naringin on cell proliferation and apoptosis in tumor tissues.The body weight,food and water intake of mice were recorded,and the major organs in different treatment groups were weighed on the last day and stained with hematoxylin and eosin for histological analysis.Meanwhile,the routine blood indicators were recorded.Results CCK-8 and annexin V-FITC/PI results confirmed that naringin(100,200,and 400µg/mL)could inhibit proliferation and promote apoptosis.The scratch wound assay and transwell migration assay results confirmed the inhibitory activity of naringin against CRC cells migration.In vivo results demonstrated the inhibitory effect of naringin on tumor growth with good bio-compatibility.Conclusion Naringin inhibited colorectal carcinogenesis by inhibiting viability of CRC cells.展开更多
MSC transplantation has been explored as a new clinical approach to stem cell-based therapies for bone diseases in regenerative medicine due to their osteogenic capability. However, only a small population of implante...MSC transplantation has been explored as a new clinical approach to stem cell-based therapies for bone diseases in regenerative medicine due to their osteogenic capability. However, only a small population of implanted MSC could successfully reach the injured areas. Therefore, enhancing MSC migration could be a beneficial strategy to improve the therapeutic potential of cell transplantation. Catharmus tinctorius volatile oil(CTVO) was found to facilitate MSC migration. Further exploration of the underlying molecular mechanism participating in the pro-migratory ability may provide a novel strategy to improve MSC transplantation efficacy. This study indicated that CTVO promotes MSC migration through enhancing ROCK2 mRNA and protein expressions. MSC migration induced by CTVO was blunted by ROCK2 inhibitor, which also decreased myosin light chain(MLC) phosphorylation.Meanwhile, the si RNA for ROCK2 inhibited the effect of CTVO on MSC migration ability and attenuated MLC phosphorylation,suggesting that CTVO may promote BMSC migration via the ROCK2/MLC signaling. Taken together, this study indicates that C.tinctorius volatile oil could enhance MSC migration via ROCK2/MLC signaling in vitro. C. tinctorius volatile oil-targeted therapy could be a beneficial strategy to improve the therapeutic potential of cell transplantation for bone diseases in regenerative medicine.展开更多
Objective:Tocompare the clinical efficacyand safety of oral administration of BuxueYimuPills(BYP),ferrous sulfate(FS),and the combination of BYP and FS on gynecological anemia,and investigate the mechanisms using netw...Objective:Tocompare the clinical efficacyand safety of oral administration of BuxueYimuPills(BYP),ferrous sulfate(FS),and the combination of BYP and FS on gynecological anemia,and investigate the mechanisms using network pharmacology.Methods:A randomized,controlled,multi-center clinical trial was conducted.Totally 150 patients with hemoglobin of 70-110 g/L due to gynecological conditions were recruited and randomized(using the block randomization method)into Buxue Yimu Pills group(24 g/d),oral iron group(FS Tablets,0.9 g/d),and combined treatment group(BYP,24g/d plus FS Tablets,0.9 g/d),50 patients in each group.At the enrollment and 4-week treatment,complete blood count,serum iron indexes were evaluated.Adverse events,liver and renal functions,as well as blood coagulation were observed.Network pharmacology was conducted to identify the active ingredients and explore the potential mechanisms of BYP.Results:Ten(20%)and 7(14%)participants discontinued the therapy due to gastrointestinal symptoms in oral iron and combination treatment groups.All 3 groups showed elevated hemoglobin.The patients in the iron group exhibited typically elevated in serum iron and ferritin and decreased in total iron-binding capacity.No change in iron indexes was observed in BYP group.The patients in the combination treatment group neither showed significant changes in serum ferritin nortotal iron-binding capacity.No significant adverse reactions were observed in the BYP group.The network pharmacology identified 27 bioactive compounds and 145 targets of BYP on gynecological anemia.Biological processes and pathways including regulation of inflammation,hormone,angiogenesis and hemostasis,responsetodecreased oxygen levels,effects on myeloma cell,and responseto metal ions were identified.Conclusion:BYP contributes to the practical improvement on gynecological anemia potentially through multi-target mechanisms and optimized iron re-distribution.展开更多
文摘Recently,a number of reports about pangolin have become hot news:The Chinese Pharmacopoeia(2020 edition)has not continued to include the drug-using standards for pangolin(Manis Squama),aristolochic(Aristolochia debilis Sieb.et Zucc),celestial vine(Fibraurea recisa Pierre),and Chinese patent drug Huanglian Yanggan pills(approval number by China State Food and Drug Administration:Z200113194).On June 5,2020,the China Forestry Administration Bureau and the Grassland Bureau co-issued an announcement to upgrade all the species of genus pangolins from the national second level of the protected wildlife to the first level.The new coronavirus(SARS-CoV-2)is also highly similar to the beta coronavirus isolated from pangolin[1].
基金supported by Graduate quality engineering project(2021CX79)and“Yifang”graduate innovation project(2022YF03).
文摘Objective:Based on the network pharmacology approach and molecular docking technology,the core targets of dihydrotanshinone I(DHT)for the treatment of helicobacter pylori(Hp)infection were searched and the potential mechanisms of drug therapy were explored.Methods:The TCMSPdatabase and Swiss Target Prediction database were employed to identify drug targets.To mine disease targets based on GeneCards,OMIM,DrugBank,DisGeNET,and TTDdatabases.Then the two were intersected to obtain common targets.The proteinproteininteraction(PPI)networkmap of common targets was constructed on the basis of the String network platform and Cytoscape software,and the targets with degree values over 1/2 maximum degree value were selected as core targets.Molecular docking verification of DHTand core targets were performed using AutoDock and PyMOL software.Finally,gene ontology(GO)functional enrichment analysis andKyoto Encyclopediaof Genes and Genomes(KEGG)pathway enrichment analysis of the common targets were carried out using the Metascape database and R-4.0.2-win software.Results:A total of 13 targets of DHTwas extracted for the treatment of Hp,and five core targets,includingSignal transducerand activator of transcription 1(STAT1),Signal transducerand activator of transcription 3(STAT3),Prostaglandin G synthase 2(PTGS2),Signal transducerand activator of transcription 4(STAT4)and Indoleamine 2,3-dioxygenase 1(IDO1),were screened according to their degree values.Molecular docking indicated that DHThad an excellent binding to the core target.29 pathways were yielded by KEGG enrichment analysis,and a total of 48 biological processes,7 cellular components and 13 molecular functions were derived from GO enrichment analysis.Conclusion:DHTmay decrease pro-inflammatory factor expression and immune cell infiltration to treat Hpinfection via the janus kinase(JAK)-signal transducer and activator of transcription(STAT)signaling pathway regulated by STAT1,STAT3,STAT4,etc.
基金General Project of Natural Science Foundation of Hunan Province(No.2021JJ30518)Science and Technology Innovation Plan Project of Hunan Provincial(No.2021SK51302)Domestic First-Class Discipline Construction Project of Chinese Medicine of Hunan University of Chinese Medicine。
文摘Objective To explore the therapeutic effect of naringin on colorectal cancer(CRC)and the related mechanism.Methods Cell counting kit-8(CCK-8)assay and annexin V-FITC/PI assay were used to detect the effect of naringin(50–400µg/mL)on cell proliferation and apoptosis of CRC cells,respectively.The scratch wound assay and transwell migration assay were used to assess the effect of naringin on CRC cell migration.Four-week-old male nude mice were injected with HCT116 cells subcutaneously to establish the tumor xenograft model.Naringin was injected intraperitoneally at 50 mg/(kg·d),with solvent and 5-fluorouracil treatment as control.The width and length of the tumors were measured and recorded every 6 days,and tumor tissues were photographed and weighed on the last day of the 24-d observation period.Immunohistochemical staining for caspase-3,proliferating cell nuclear antigen and TUNEL assay were used to evaluate the effect of naringin on cell proliferation and apoptosis in tumor tissues.The body weight,food and water intake of mice were recorded,and the major organs in different treatment groups were weighed on the last day and stained with hematoxylin and eosin for histological analysis.Meanwhile,the routine blood indicators were recorded.Results CCK-8 and annexin V-FITC/PI results confirmed that naringin(100,200,and 400µg/mL)could inhibit proliferation and promote apoptosis.The scratch wound assay and transwell migration assay results confirmed the inhibitory activity of naringin against CRC cells migration.In vivo results demonstrated the inhibitory effect of naringin on tumor growth with good bio-compatibility.Conclusion Naringin inhibited colorectal carcinogenesis by inhibiting viability of CRC cells.
基金supported by the National Nature Science Foundation of China(Nos.81503593,81273783,and 81473699)Guangdong Science and Technology Department(No.2014A020221055)the Natural Science Foundation of Guangdong Province(Nos.2016A030313649 and 2017A030313729)
文摘MSC transplantation has been explored as a new clinical approach to stem cell-based therapies for bone diseases in regenerative medicine due to their osteogenic capability. However, only a small population of implanted MSC could successfully reach the injured areas. Therefore, enhancing MSC migration could be a beneficial strategy to improve the therapeutic potential of cell transplantation. Catharmus tinctorius volatile oil(CTVO) was found to facilitate MSC migration. Further exploration of the underlying molecular mechanism participating in the pro-migratory ability may provide a novel strategy to improve MSC transplantation efficacy. This study indicated that CTVO promotes MSC migration through enhancing ROCK2 mRNA and protein expressions. MSC migration induced by CTVO was blunted by ROCK2 inhibitor, which also decreased myosin light chain(MLC) phosphorylation.Meanwhile, the si RNA for ROCK2 inhibited the effect of CTVO on MSC migration ability and attenuated MLC phosphorylation,suggesting that CTVO may promote BMSC migration via the ROCK2/MLC signaling. Taken together, this study indicates that C.tinctorius volatile oil could enhance MSC migration via ROCK2/MLC signaling in vitro. C. tinctorius volatile oil-targeted therapy could be a beneficial strategy to improve the therapeutic potential of cell transplantation for bone diseases in regenerative medicine.
文摘Objective:Tocompare the clinical efficacyand safety of oral administration of BuxueYimuPills(BYP),ferrous sulfate(FS),and the combination of BYP and FS on gynecological anemia,and investigate the mechanisms using network pharmacology.Methods:A randomized,controlled,multi-center clinical trial was conducted.Totally 150 patients with hemoglobin of 70-110 g/L due to gynecological conditions were recruited and randomized(using the block randomization method)into Buxue Yimu Pills group(24 g/d),oral iron group(FS Tablets,0.9 g/d),and combined treatment group(BYP,24g/d plus FS Tablets,0.9 g/d),50 patients in each group.At the enrollment and 4-week treatment,complete blood count,serum iron indexes were evaluated.Adverse events,liver and renal functions,as well as blood coagulation were observed.Network pharmacology was conducted to identify the active ingredients and explore the potential mechanisms of BYP.Results:Ten(20%)and 7(14%)participants discontinued the therapy due to gastrointestinal symptoms in oral iron and combination treatment groups.All 3 groups showed elevated hemoglobin.The patients in the iron group exhibited typically elevated in serum iron and ferritin and decreased in total iron-binding capacity.No change in iron indexes was observed in BYP group.The patients in the combination treatment group neither showed significant changes in serum ferritin nortotal iron-binding capacity.No significant adverse reactions were observed in the BYP group.The network pharmacology identified 27 bioactive compounds and 145 targets of BYP on gynecological anemia.Biological processes and pathways including regulation of inflammation,hormone,angiogenesis and hemostasis,responsetodecreased oxygen levels,effects on myeloma cell,and responseto metal ions were identified.Conclusion:BYP contributes to the practical improvement on gynecological anemia potentially through multi-target mechanisms and optimized iron re-distribution.
基金supported by Wang Jing-jing’s Inheritance Studio of National Famous Traditional Chinese Medicine ExpertsGraduate Student Research Innovation Project of Hunan Province,No.CX2016B354Open Fund for National Key Discipline of Diagnostics of Traditional Chinese Medicine,No.2015ZYZD27~~
