Clinical observation of colon carcinoma treated with Boerning capsules plus FOLFOX4 regimen

Objective:To evaluate the effects and adverse reactions of Boerning capsules plus FOLFOX4 regimen in clinical treatment for colon carcinoma.Methods:Total patients with colon carcinoma were randomly divided into two groups from January 2005 to May 2006:group A(45 patients)were received Boerning capsules plus FOLFOX4 regimen treatment,and group B(49 patients)were received FOLFOX4 regimen treatment only.Results:Boerning capsules could obviously improve the life quality of the colon carcinoma patients.The weight gain in group A was higher than that of group B.No significant existed in the body immune function changes(T cell subgroup).But it could ameliorate the adverse reactions which induced by the chemotherapy.Conclusion:Boerning capsules can improve the life quality of the colon carcinoma patients and ame- liorate the symptoms and sign which induced by the chemotherapy.

Effects of genistein and 17 beta-estradiol on hippocampal synaptophysin expression in ovariectomized rats

BACKGROUND： Phytoestrogen, derived from plants, is an estrogen-like element, and is effective and safe for estrogen replacement. OBJECTIVE： To compare the interventional effects of genistein and 17 S-estradiol on learning and memory and synaptophysin （SYN） expression in the hippocampus of ovariectomized rats.DESIGN： Randomized controlled animal study.SETTING： Department of Neurology, the Third Affiliated Hospital, Xiangya Medical College, Central South University. MATERIALS： 130 healthy female Sprague Dawley （SD） rats, 6 months old and weighing （293.1 ± 10.2） g, were provided by the Second Xiangya Hospital of Central South University. This animal experiment received confirmed consent from the local ethics committee. All rats were randomly divided into 5 groups, including baseline group （n= 10）, sham operation group （n = 30）, ovariectomized group （n = 30）, genistein group （n = 30）, and 17 β -estradiol group （n = 30）. Rats in the latter four groups were observed for 3 weeks （n = 10） and for 15 weeks （n = 20） after model establishment. METHODS： This study was performed at the Department of Endocrinology, the Second Affiliated Hospital, Xiangya Medical College, Central South University from August 2005 to January 2006. Animals were not submitted to any treatment in the baseline group, but anesthetized and sacrificed at the 7 months of age. After anesthesia in the ovariectomized, genistein, and 17 S-estradiol groups, both ovaries were separated and resected to establish an ovariectomized model. The same volume of fat was resected in the sham operation group. After surgery, rats were intraperitoneally injected with 5 mg/kg genistein in the genistein group,10μg/kg 17 β -estradiol in the 17 β-estradiol group, and 0.1 mL/100 g dimethyl sulfoxide （DMSO）/polyethylene glycol （PEG）-200 stock solution in the sham operation and ovariectomized groups once a day until one day before sacrifice. MAIN OUTCOME MEASURES：① Learning and memory changes of SD rats were detected using water maze behavioral testing 3 and 15 weeks after surgery. ② SYN expression in the hippocampus was measured using immunohistochemistry. RESULTS： A total of 16 out of 130 rats died due to infection, and 114 rats were included in the final analysis. ① Comparison of water maze results from the five groups： by 3 and 15 weeks after surgery, escape latency was prolonged and platform-crossing times decreased in the ovariectomized group compared to the baseline, genistein, 17 β-estradiol, and sham operation groups （t = 4.17 14.64, P 〈 0.05）. However, there were no significant differences in escape latency and platform-crossing times among the sham operation, genistein, and 17 S-estradiol groups （P 〉 0.05）. ② Distribution and quantity of SYN immunoreactive products in hippocampus： SYN-immunoreactive cells stained darkly in the baseline and sham operation groups, but were lightly stained in the genistein, 17 S -estradiol, and ovariectomized groups. In particular, SYN-immunoreactive cells stained lightly in the ovariectomized group 15 weeks after surgery. SYN correction gray values in hippocampal sub-regions, especially in the mossy fiber layer of the CA3 region, of the ovariectomized group was lower compared to the baseline, sham operation, 17 β -estradiol, and genistein groups （t = 12.57 23.92, P 〈 0.05） 15 weeks after surgery. However, there were no significant differences in SYN correction gray values among the baseline, sham operation, 17 β -estradiol and genistein groups （P 〉 0.05）. CONCLUSION： Genistein or 17 β -estradiol supplemental therapy antagonizes memory deterioration, due to endogenous estrogen deficiency and blocks the decrease of SYN expression in the hippocampus. The effect of genistein is similar to 17 β -estradiol.

Clinical analysis of 13 cases of synchronous gastric and colorectal cancer

Objective:To investigate the clinical characteristics and prognosis of patients with synchronous gastric and colorectal cancer.Methods:Clinical and pathological data of 3416 cases with gastric cancer and 3109 with colorectal cancer, from March 1985 to May 2005,were analyzed retrospectively.Results:Thirteen cases were confirmed as synchronous gastric and colorectal cancer and the incidences of the disease were 0.38%in gastric cancer cases and 0.42%in colorectal cancer.Of patients suffering from synchronous gastric and colorectal cancer,15.4%were diagnosed before the first operation and 33.3%were not diagnosed with the second cancer until it was radically dissected.The 3-year survival rate of the patients was 30.8%.Conclusion:The preoperative diagnosis rate of the synchronous gastric and colorectal cancer,the radical dis- section rate of the second cancer and the 3-year survival rate after surgical removal of the second cancer were rather low.The key for enhancing the radical dissection rate of the second cancer and the survival rate after surgery for the second cancer lies in the improvement of diagnosis rate before the first operation.

Study of Tripterygium Associated with Nicotinamide in Treating Late-onset Autoimmune Diabetes Mellitus in Adults

Objective: To explore the effect of Tripterygium polyglycoside (TP) associated with nicotinamide on the islet cell function, immune parameters and lipoperoxide (LPO) in adult patients with late-onset autoimmune diabetes mellitus (LADA). Methods: Thirty-six cases of LADA were randomly divided into three groups: TP group (n=12), treated with TP plus orally taken metformin; combined treatment group (n= 12), treated with TP combined with nicotinamide and metformin, and control group (n=12) treated with metformin alone. They were followed-up for 18 months. Results: (1) Compared with the control group after 9 months of treatment, postprandial plasma glucose and LPO in combined treatment group were decreased (P <0.05), and the postprandial C-peptide was higher (P<0.05). At the 18th month, the value of postprandial C-peptide in the TP and combined treatment group was higher than that in the control group. The slL-2R level of both TP and combined treatment groups were lowered (P<0.01); (2) Islet cell antibody (ICA) positive of 5 cases in the TP group and 6 cases in the combined treatment group got converted to the negative respectively , while only one in the control group at the time (P<0.05); (3) The level of LPO in the combined treatment group was significantly lower than that in the TP group at the 18th month of treatment (P<0. 05). Conclusion: TP combined with nicotinamide played a role in immunity regulation, decreasing the titer of islet cell antibody and slL-2R, which also reduced the production of LPO and had a tendency to improve islet cell function in early LADA patients.