Bacillus subtilis is the focus of both academic and industrial research.Previous studies have reported a number of sequence variations in different B.subtilis strains.To uncover the genetic variation and evolutionary ...Bacillus subtilis is the focus of both academic and industrial research.Previous studies have reported a number of sequence variations in different B.subtilis strains.To uncover the genetic variation and evolutionary pressure in B.subtilis strains,we performed whole genome sequencing of two B.subtilis isolates,KM and CGMCC63528.Comparative genomic analyses of these two strains with other B.subtilis strains identified high展开更多
A variety of techniques have been used for treating avascular necrosis of the femoral head(ANFH),but have frequently failed.In this study,we proposed aβ-TCP system for the treatment of ANFH by boosting revascularizat...A variety of techniques have been used for treating avascular necrosis of the femoral head(ANFH),but have frequently failed.In this study,we proposed aβ-TCP system for the treatment of ANFH by boosting revascularization and bone regeneration.The angio-conductive properties and concurrent osteogenesis of the highly interconnected porousβ-TCP scaffold were revealed and quantified through an in vivo model that simulated the ischemic environment of ANFH.Mechanical test and finite element analysis showed that the mechanical loss caused by tissue necrosis and surgery was immediately partially compensated after implantation,and the strength of the operated femoral head was adaptively increased and eventually returned to normal bone,along with continuous material degradation and bone regeneration.For translational application,we further conducted a multi-center open-label clinical trial to assess the efficacy of theβ-TCP system in treating ANFH.Two hundred fourteen patients with 246 hips were enrolled for evaluation,and 82.1%of the operated hips survived at a 42.79-month median follow-up.The imaging results,hip function,and pain scores were dramatically improved compared to preoperative levels.ARCO stage II disease outperformed stage III in terms of clinical effectiveness.Thus,bio-adaptive reconstruction using theβ-TCP system is a promising hip-preserving strategy for the treatment of ANFH.展开更多
基金supported by Significant new drugs creation,12th Five-Year plan special science and Technology Major(2013ZX09304101)the National High Technology Research and Development Program of China(863 Program)(2014AA022210)
文摘Bacillus subtilis is the focus of both academic and industrial research.Previous studies have reported a number of sequence variations in different B.subtilis strains.To uncover the genetic variation and evolutionary pressure in B.subtilis strains,we performed whole genome sequencing of two B.subtilis isolates,KM and CGMCC63528.Comparative genomic analyses of these two strains with other B.subtilis strains identified high
文摘A variety of techniques have been used for treating avascular necrosis of the femoral head(ANFH),but have frequently failed.In this study,we proposed aβ-TCP system for the treatment of ANFH by boosting revascularization and bone regeneration.The angio-conductive properties and concurrent osteogenesis of the highly interconnected porousβ-TCP scaffold were revealed and quantified through an in vivo model that simulated the ischemic environment of ANFH.Mechanical test and finite element analysis showed that the mechanical loss caused by tissue necrosis and surgery was immediately partially compensated after implantation,and the strength of the operated femoral head was adaptively increased and eventually returned to normal bone,along with continuous material degradation and bone regeneration.For translational application,we further conducted a multi-center open-label clinical trial to assess the efficacy of theβ-TCP system in treating ANFH.Two hundred fourteen patients with 246 hips were enrolled for evaluation,and 82.1%of the operated hips survived at a 42.79-month median follow-up.The imaging results,hip function,and pain scores were dramatically improved compared to preoperative levels.ARCO stage II disease outperformed stage III in terms of clinical effectiveness.Thus,bio-adaptive reconstruction using theβ-TCP system is a promising hip-preserving strategy for the treatment of ANFH.
