Aim: To investigate the activity of RRR-α-tocopheryloxybutyric acid (TOB), an ether analog of RRR-α-tocopheryl succinate (VES), in prostate cancer cells. Methods: VES and TOB were used to treat prostate cancer...Aim: To investigate the activity of RRR-α-tocopheryloxybutyric acid (TOB), an ether analog of RRR-α-tocopheryl succinate (VES), in prostate cancer cells. Methods: VES and TOB were used to treat prostate cancer LNCaP, PC3, and 22Rvl cells and primary-cultured prostate fibroblasts. The proliferation rates were determined by MTT assay, the cell viabilities were determined by trypan blue exclusion assay, and the cell deaths were evaluated by using Cell Death Detection ELISA kit. The protein expression levels were determined by Western blot analysis. Results: The MTT growth assay demonstrated that TOB could effectively suppress the proliferation of prostate cancer cells, but not normal prostate fibroblasts. Mechanism dissections revealed that TOB reduced cell viability and induced apoptosis in prostate cancer cells similar to VES. In addition, both TOB and VES suppressed prostate-specific antigen (PSA) at the transcriptional level leading to reduced PSA protein expression. Furthermore, vitamin D receptor (VDR) expression increased after the addition of TOB. Conclusion: Our data suggests that the VES derivative, TOB, is effective in inhibiting prostate cancer cell proliferation, suggesting that TOB could be used for both chemopreventive and chemotherapeutic purposes in the future.展开更多
BACKGROUND Ulcerative colitis(UC)is considered to be closely associated with alteration of intestinal microorganisms.According to the traditional Chinese medicine(TCM)theory,UC can be divided into two disease syndrome...BACKGROUND Ulcerative colitis(UC)is considered to be closely associated with alteration of intestinal microorganisms.According to the traditional Chinese medicine(TCM)theory,UC can be divided into two disease syndromes called Pi-Xu-Shi-Yun(PXSY)and Da-Chang-Shi-Re(DCSR).The relationships among gut microbiota,TCM syndromes,and UC pathogenesis have not been well investigated.AIM To investigate the role of gut microbiota in UC and the distinction of microbiota dysbiosis between PXSY and DCSR syndromes.METHODS From May 2015 to February 2016,UC patients presenting to LongHua Hospital who met the established inclusion and exclusion criteria were enrolled in this retrospective study.Fresh stool specimens of UC patients with PXSY or DCSR were collected.The feces of the control group came from the health examination population of Longhua Hospital.The composition of gut bacterial communities in stool samples was determined by the pyrosequencing of 16S ribosomal RNA.The high-throughput sequencing reads were processed with QIIME,and biological functions were predicted using Phylogenetic Investigation of Communities by Reconstruction of Unobserved States.RESULTS The composition of gut bacterial communities in 93 stool samples(30 healthy controls,32 patients with PXSY syndrome,and 31 patients with DCSR syndrome)was determined by the pyrosequencing of 16S ribosomal RNA.Beta diversity showed that the composition of the microbiota was different among the three groups.At the family level,Porphyromonadaceae,Rikeneliaceae,and Lachnospiraceae significantly decreased while Enterococcus,Streptococcus,and other potential pathogens significantly increased in UC patients compared to healthy subjects.At the genus level,Parabacteroides,Dorea,and Ruminococcus decreased while Faeca-libacterium showed increased abundance in UC compared to healthy controls.Five differential taxa were identified between PXSY and DCSR syndromes.At the genus level,a significantly increased abundance of Streptococcus was observed in DCSR patients,while Lachnoclostridium increased in PXSY patients.The differential functional pathways of the gut microbiome between the PXSY and DCSR groups mainly included lipid metabolism,immunity,and the metabolism of polypeptides.CONCLUSION Our study suggests that the gut microbiota contributes to the distinction between the two TCM syndromes of UC.展开更多
Here we have presented a sensitive and selective LC-MS/MS method for the quantification of tyrphostin A9, which is a selective inhibitor for platelet derived growth factor receptor tyrosine kinase and has been investi...Here we have presented a sensitive and selective LC-MS/MS method for the quantification of tyrphostin A9, which is a selective inhibitor for platelet derived growth factor receptor tyrosine kinase and has been investigated in vitro as a potent oxidative phosphorylation uncoupler. The murine analytical method was developed for three biological matrices: cell culture media, 3T3-L1 cell lysate, and murine plasma. For each matrix the limit of detection and the limit of quantification were found to be 0.5 ng/mL and 1.0 ng/ mL, respectively. The range of standard curve for each matrix was 1.0-100 ng/mL, linearity was >0.99, and the precision and accuracy were within 20%. 3-(3,5-di-tert-butyl-4-hydroxyphenyl) propanoic acid was found to be the most suitable internal standard. The validated LC-MS/MS method was used to investigate stability and in vitro pharmacokinetics of tyrphostin A9. It was found that tyrphostin A9 is susceptible to hydrolysis, and the degradation product was identified as 3,5-di-tert-butyl-4- hydroxybenzaldehyde. Tyrphostin A9 was not stable in biological matrices, and the rate of its degradation in murine plasma was faster than that in cell culture media. In vitro pharmacokinetic studies revealed that tyrphostin A9 concentrations in the cell culture media declined in a bi-exponential manner and the concentrations inside the adipocytes remained constant, suggesting tyrphostin A9 has an intracellular binding site and is retained within the cell. The LC-MS/MS method presented here paves the way for further quantitative investigations involving tyrphostin A9.展开更多
In order to secure the massive heterogeneous medical data for the complex scenarios and improve the information sharing efficiency in healthcare system,a distributed medical data ledger model(DMDL)is proposed in this ...In order to secure the massive heterogeneous medical data for the complex scenarios and improve the information sharing efficiency in healthcare system,a distributed medical data ledger model(DMDL)is proposed in this paper.This DMDL model has adopted the blockchain technology including the function decoupling,the distributed consensus,smart contract as well as multi-channel communication structure of consortium blockchain.The DMDL model not only has high adaptability,but also meets the requirements of the medical treatment processes which generally involve multientities,highly private information and secure transaction.The steps for processing the medical data are also introduced.Additionally,the methods for the definition and application of the DMDL model are presented for three specific medical scenarios,i.e.,the management of the heterogeneous data,copyright protection for medical data and the secure utilization of sensitive data.The advantage of the proposed DMDL model is demonstrated by comparing with the models which are being currently adopted in healthcare system.展开更多
Background and Purpose:Nicotine addiction and abuse remains a global health issue.To date,the fundamental neurobiological mechanism of nicotine addiction remains incompletely understood.Trace amine-associated receptor...Background and Purpose:Nicotine addiction and abuse remains a global health issue.To date,the fundamental neurobiological mechanism of nicotine addiction remains incompletely understood.Trace amine-associated receptor 1(TAAR1)is thought to directly modulate dopaminergic system and are thought to be a neural substrate underlying addictivelike behaviors.We aimed to investigate the role of TAAR1 in nicotine addictive-like behaviors.Experimental Approach:TAAR1 expression after nicotine treatment was evaluated by western blotting.c-Fos immunofluorescence and in vivo fast-scan cyclic voltammetry were used to examine the activation of brain regions and dopamine release,respectively.We then thoroughly and systematically examined the role of TAAR1 in mediating nicotine-induced sensitization,nicotine discrimination,nicotine self-administration,nicotine demand curve,and the reinstatement of nicotine-seeking.Local pharmacological manipulation was conducted to determine the role of TAAR1 in the nucleus accumbens(NAcs)in the reinstatement of nicotine-seeking.Minipump was implanted into TAAR 1 knockout mice and their wildtype counterparts to establish physical dependence.One week after the minipump implantation,the pump was retrieved and mecamylamine was used to precipitate physical withdrawal signs including observable signs and anxiety-like behaviors(elevated plus maze).Key Results:We found that the expression of TAAR1 protein was selectively downregulated in the NAc,with no change in either dorsal striatum or prefrontal cortex.TAAR1 activation was sufficient to block nicotine-induced c-Fos expression in the NAc,while also reducing nicotine-induced dopamine release in the NAc.Systemic administration of TAAR1 agonists attenuated the expression and development of nicotine-induced sensitization,nicotine self-administration,the reinstatement of nicotine-seeking,and increased the elasticity of nicotine demand curve,while intra-NAc infusions of a TAAR1 agonist was sufficient to attenuate nicotine reinstatement.Moreover,TAAR1-knockout rats showed augmented cue-induced and druginduced reinstatement of nicotine-seeking.Mice that were physically dependent on chronic nicotine exposure demonstrated significant withdrawal signs after mecamylamine treatment,and TAAR 1 knockout mice demonstrated more severe withdrawal signs.TAAR 1 agonist reduced the withdrawal signs in the wildtype mice.Conclusions and Implications:These results indicated that modulation of TAAR1 activity regulates nicotine addictive-like behaviors and TAAR1 represents a novel target towards the treatment of nicotine addiction.展开更多
Recent work suggests a link betweenα-synuclein(α-syn)and mitochondrial dysfunction;however,the mechanisms of howα-syn influences mitochondrial function are still unclear.Most notably,whetherα-syn plays a direct ro...Recent work suggests a link betweenα-synuclein(α-syn)and mitochondrial dysfunction;however,the mechanisms of howα-syn influences mitochondrial function are still unclear.Most notably,whetherα-syn plays a direct role during mitochondrial function and/or whether diseasedα-syn-mediated mitochondrial dysfunction is a potential modifiable risk factor in Parkinson’s disease(PD)is unknown.To date,mutations in more than eight genes cause familial PD(fPD)and have functions in diverse pathways including synaptic homeostasis,mitochondria maintenance,autophagy/lysosome,and ubiquitin-proteasome pathways.展开更多
Styrene is extensively used in industry,but its ototoxicity,in particular in the pregnant female and the offspring,is still not well understood.In the current study,young adult male rats and pregnant female rats were ...Styrene is extensively used in industry,but its ototoxicity,in particular in the pregnant female and the offspring,is still not well understood.In the current study,young adult male rats and pregnant female rats were exposed to styrene by gavage at different doses.The young adult male rats received a total of 12g/kg styrene within different periods (800 mg/kg/day for 5 days/week for 3 weeks,400 mg/kg/day for 5 days/week for 6 weeks,200 mg/kg/day for 5 days/week for 12 weeks,and 100 mg/kg/day for 5 days/week for 24 weeks) and the pregnant female rats received styrene at a dose of 400 mg/kg/day for 5 days/week for 6 weeks starting from the gestation day-4.Hearing loss and hair cell loss were assessed 5 days after the styrene treatment in the young adult male rats and in the mother rats.The cochlear impairments in the rat pups were examined 2 months after their birth.The styrene exposure caused hearing loss and hair cell loss starting from the mid-frequency region in the third row of outer hair cells (OHCs) and the impairments appeared to be related to the dosing level in each single day.Significantly,the styrene exposure to the pregnant rats interfered with auditory functional development of their fetus,leading to a deficit of cochlear amplification,although the OHCs appeared to develop well.The results indicate that a short-term high-level styrene exposure may be more ototoxic than a long-term low-level exposure for a similar total styrene dose and the styrene in the pregnant woman’s body may interfere with auditory development of their fetus.展开更多
With promising applications in e-health and entertainment, wireless body area networks (WBANs) have attracted the interest of both academia and industry. If WBANs are densely deployed within a small area, serious pr...With promising applications in e-health and entertainment, wireless body area networks (WBANs) have attracted the interest of both academia and industry. If WBANs are densely deployed within a small area, serious problems may arise between the WBANs. In this paper, we discuss issues related to the coexistence of WBANs and investigate the main factors that cause inter-WBAN interference. We survey inter- WBAN interference mitigation strategies and track recent research developments. We also discuss unresolved issues related to inter-WBAN interference mitigation and propose fu- ture research directions.展开更多
Subwavelength manipulation of light waves with high precision can enable new and exciting applications in spectroscopy,sensing,and medical imaging.For these applications,miniaturized spectrometers are desirable to ena...Subwavelength manipulation of light waves with high precision can enable new and exciting applications in spectroscopy,sensing,and medical imaging.For these applications,miniaturized spectrometers are desirable to enable the on-chip analysis of spectral information.In particular,for imaging-based spectroscopic sensing mechanisms,the key challenge is to determine the spatial-shift information accurately(i.e.,the spatial displacement introduced by wavelength shift or biological or chemical surface binding),which is similar to the challenge presented by super-resolution imaging.Here,we report a unique"rainbow"trapping metasurface for on-chip spectrometers and sensors.Combined with super-resolution image processing,the low-setting 4×optical microscope system resolves a displacement of the resonant position within 35 nm on the plasmonic rainbow trapping metasurface with a tiny area as small as0.002 mm2.This unique feature of the spatial manipulation of efficiently coupled rainbow plasmonic resonances reveals a new platform for miniaturized on-chip spectroscopic analysis with a spectral resolution of 0.032 nm in wavelength shift.Using this low-setting 4×microscope imaging system,we demonstrate a biosensing resolution of 1.92×109exosomes per milliliter for A549-derived exosomes and distinguish between patient samples and healthy controls using exosomal epidermal growth factor receptor(EGFR)expression values,thereby demonstrating a new on-chip sensing system for personalized accurate bio/chemical sensing applications.展开更多
The development of global informatization and its integration with industrialization symbolizes that human society has entered into the big data era.This article covers seven new characteristics of Geomatics(i.e.ubiqu...The development of global informatization and its integration with industrialization symbolizes that human society has entered into the big data era.This article covers seven new characteristics of Geomatics(i.e.ubiquitous sensor,multi-dimensional dynamics,integration via networking,full automation in real time,from sensing to recognition,crowdsourcing and volunteered geographic information,and serviceoriented science),and puts forward the corresponding critical technical challenges in the construction of integrated space-air-ground geospatial networks.Through the discussions outlined in this paper,we propose a new development stage of Geomatics entitled‘Connected Geomatics,’which is defined as a multi-disciplinary science and technology that uses systematic approaches and integrates methods of spatio-temporal data acquisition,information extraction,network management,knowledge discovery,and spatial sensing and recognition,as well as intelligent location-based services pertaining to any physical objects and human activities on the earth.It is envisioned that the advancement of Geomatics will make a great contribution to human sustainable development.展开更多
Recent experiments demonstrated that chiral symmetry breaking at an exceptional point(EP) is a viable route to achieve unidirectional laser emission in microring lasers. By a detailed semiconductor laser rate equation...Recent experiments demonstrated that chiral symmetry breaking at an exceptional point(EP) is a viable route to achieve unidirectional laser emission in microring lasers. By a detailed semiconductor laser rate equation model,we show here that unidirectional laser emission at an EP is a robust regime. Slight deviations from the EP condition can break preferential unidirectional lasing near threshold via a Hopf instability. However, abovea "second" laser threshold, unidirectional emission is restored.展开更多
文摘Aim: To investigate the activity of RRR-α-tocopheryloxybutyric acid (TOB), an ether analog of RRR-α-tocopheryl succinate (VES), in prostate cancer cells. Methods: VES and TOB were used to treat prostate cancer LNCaP, PC3, and 22Rvl cells and primary-cultured prostate fibroblasts. The proliferation rates were determined by MTT assay, the cell viabilities were determined by trypan blue exclusion assay, and the cell deaths were evaluated by using Cell Death Detection ELISA kit. The protein expression levels were determined by Western blot analysis. Results: The MTT growth assay demonstrated that TOB could effectively suppress the proliferation of prostate cancer cells, but not normal prostate fibroblasts. Mechanism dissections revealed that TOB reduced cell viability and induced apoptosis in prostate cancer cells similar to VES. In addition, both TOB and VES suppressed prostate-specific antigen (PSA) at the transcriptional level leading to reduced PSA protein expression. Furthermore, vitamin D receptor (VDR) expression increased after the addition of TOB. Conclusion: Our data suggests that the VES derivative, TOB, is effective in inhibiting prostate cancer cell proliferation, suggesting that TOB could be used for both chemopreventive and chemotherapeutic purposes in the future.
基金Supported by the National Natural Science Foundation of China,No.81704009,No.81873253,No.81573892,and No.81770571the Project of Shanghai Municipal Health and Family Planning Commission,No.201640122
文摘BACKGROUND Ulcerative colitis(UC)is considered to be closely associated with alteration of intestinal microorganisms.According to the traditional Chinese medicine(TCM)theory,UC can be divided into two disease syndromes called Pi-Xu-Shi-Yun(PXSY)and Da-Chang-Shi-Re(DCSR).The relationships among gut microbiota,TCM syndromes,and UC pathogenesis have not been well investigated.AIM To investigate the role of gut microbiota in UC and the distinction of microbiota dysbiosis between PXSY and DCSR syndromes.METHODS From May 2015 to February 2016,UC patients presenting to LongHua Hospital who met the established inclusion and exclusion criteria were enrolled in this retrospective study.Fresh stool specimens of UC patients with PXSY or DCSR were collected.The feces of the control group came from the health examination population of Longhua Hospital.The composition of gut bacterial communities in stool samples was determined by the pyrosequencing of 16S ribosomal RNA.The high-throughput sequencing reads were processed with QIIME,and biological functions were predicted using Phylogenetic Investigation of Communities by Reconstruction of Unobserved States.RESULTS The composition of gut bacterial communities in 93 stool samples(30 healthy controls,32 patients with PXSY syndrome,and 31 patients with DCSR syndrome)was determined by the pyrosequencing of 16S ribosomal RNA.Beta diversity showed that the composition of the microbiota was different among the three groups.At the family level,Porphyromonadaceae,Rikeneliaceae,and Lachnospiraceae significantly decreased while Enterococcus,Streptococcus,and other potential pathogens significantly increased in UC patients compared to healthy subjects.At the genus level,Parabacteroides,Dorea,and Ruminococcus decreased while Faeca-libacterium showed increased abundance in UC compared to healthy controls.Five differential taxa were identified between PXSY and DCSR syndromes.At the genus level,a significantly increased abundance of Streptococcus was observed in DCSR patients,while Lachnoclostridium increased in PXSY patients.The differential functional pathways of the gut microbiome between the PXSY and DCSR groups mainly included lipid metabolism,immunity,and the metabolism of polypeptides.CONCLUSION Our study suggests that the gut microbiota contributes to the distinction between the two TCM syndromes of UC.
基金supported by NIH grant GM114179 and AI138195 to D.K.S.the Centre for Protein Therapeutics at University at BuffaloDonald F. and Edna G. Bishop Scholarship Foundation for their continued support
文摘Here we have presented a sensitive and selective LC-MS/MS method for the quantification of tyrphostin A9, which is a selective inhibitor for platelet derived growth factor receptor tyrosine kinase and has been investigated in vitro as a potent oxidative phosphorylation uncoupler. The murine analytical method was developed for three biological matrices: cell culture media, 3T3-L1 cell lysate, and murine plasma. For each matrix the limit of detection and the limit of quantification were found to be 0.5 ng/mL and 1.0 ng/ mL, respectively. The range of standard curve for each matrix was 1.0-100 ng/mL, linearity was >0.99, and the precision and accuracy were within 20%. 3-(3,5-di-tert-butyl-4-hydroxyphenyl) propanoic acid was found to be the most suitable internal standard. The validated LC-MS/MS method was used to investigate stability and in vitro pharmacokinetics of tyrphostin A9. It was found that tyrphostin A9 is susceptible to hydrolysis, and the degradation product was identified as 3,5-di-tert-butyl-4- hydroxybenzaldehyde. Tyrphostin A9 was not stable in biological matrices, and the rate of its degradation in murine plasma was faster than that in cell culture media. In vitro pharmacokinetic studies revealed that tyrphostin A9 concentrations in the cell culture media declined in a bi-exponential manner and the concentrations inside the adipocytes remained constant, suggesting tyrphostin A9 has an intracellular binding site and is retained within the cell. The LC-MS/MS method presented here paves the way for further quantitative investigations involving tyrphostin A9.
文摘In order to secure the massive heterogeneous medical data for the complex scenarios and improve the information sharing efficiency in healthcare system,a distributed medical data ledger model(DMDL)is proposed in this paper.This DMDL model has adopted the blockchain technology including the function decoupling,the distributed consensus,smart contract as well as multi-channel communication structure of consortium blockchain.The DMDL model not only has high adaptability,but also meets the requirements of the medical treatment processes which generally involve multientities,highly private information and secure transaction.The steps for processing the medical data are also introduced.Additionally,the methods for the definition and application of the DMDL model are presented for three specific medical scenarios,i.e.,the management of the heterogeneous data,copyright protection for medical data and the secure utilization of sensitive data.The advantage of the proposed DMDL model is demonstrated by comparing with the models which are being currently adopted in healthcare system.
文摘Background and Purpose:Nicotine addiction and abuse remains a global health issue.To date,the fundamental neurobiological mechanism of nicotine addiction remains incompletely understood.Trace amine-associated receptor 1(TAAR1)is thought to directly modulate dopaminergic system and are thought to be a neural substrate underlying addictivelike behaviors.We aimed to investigate the role of TAAR1 in nicotine addictive-like behaviors.Experimental Approach:TAAR1 expression after nicotine treatment was evaluated by western blotting.c-Fos immunofluorescence and in vivo fast-scan cyclic voltammetry were used to examine the activation of brain regions and dopamine release,respectively.We then thoroughly and systematically examined the role of TAAR1 in mediating nicotine-induced sensitization,nicotine discrimination,nicotine self-administration,nicotine demand curve,and the reinstatement of nicotine-seeking.Local pharmacological manipulation was conducted to determine the role of TAAR1 in the nucleus accumbens(NAcs)in the reinstatement of nicotine-seeking.Minipump was implanted into TAAR 1 knockout mice and their wildtype counterparts to establish physical dependence.One week after the minipump implantation,the pump was retrieved and mecamylamine was used to precipitate physical withdrawal signs including observable signs and anxiety-like behaviors(elevated plus maze).Key Results:We found that the expression of TAAR1 protein was selectively downregulated in the NAc,with no change in either dorsal striatum or prefrontal cortex.TAAR1 activation was sufficient to block nicotine-induced c-Fos expression in the NAc,while also reducing nicotine-induced dopamine release in the NAc.Systemic administration of TAAR1 agonists attenuated the expression and development of nicotine-induced sensitization,nicotine self-administration,the reinstatement of nicotine-seeking,and increased the elasticity of nicotine demand curve,while intra-NAc infusions of a TAAR1 agonist was sufficient to attenuate nicotine reinstatement.Moreover,TAAR1-knockout rats showed augmented cue-induced and druginduced reinstatement of nicotine-seeking.Mice that were physically dependent on chronic nicotine exposure demonstrated significant withdrawal signs after mecamylamine treatment,and TAAR 1 knockout mice demonstrated more severe withdrawal signs.TAAR 1 agonist reduced the withdrawal signs in the wildtype mice.Conclusions and Implications:These results indicated that modulation of TAAR1 activity regulates nicotine addictive-like behaviors and TAAR1 represents a novel target towards the treatment of nicotine addiction.
文摘Recent work suggests a link betweenα-synuclein(α-syn)and mitochondrial dysfunction;however,the mechanisms of howα-syn influences mitochondrial function are still unclear.Most notably,whetherα-syn plays a direct role during mitochondrial function and/or whether diseasedα-syn-mediated mitochondrial dysfunction is a potential modifiable risk factor in Parkinson’s disease(PD)is unknown.To date,mutations in more than eight genes cause familial PD(fPD)and have functions in diverse pathways including synaptic homeostasis,mitochondria maintenance,autophagy/lysosome,and ubiquitin-proteasome pathways.
文摘Styrene is extensively used in industry,but its ototoxicity,in particular in the pregnant female and the offspring,is still not well understood.In the current study,young adult male rats and pregnant female rats were exposed to styrene by gavage at different doses.The young adult male rats received a total of 12g/kg styrene within different periods (800 mg/kg/day for 5 days/week for 3 weeks,400 mg/kg/day for 5 days/week for 6 weeks,200 mg/kg/day for 5 days/week for 12 weeks,and 100 mg/kg/day for 5 days/week for 24 weeks) and the pregnant female rats received styrene at a dose of 400 mg/kg/day for 5 days/week for 6 weeks starting from the gestation day-4.Hearing loss and hair cell loss were assessed 5 days after the styrene treatment in the young adult male rats and in the mother rats.The cochlear impairments in the rat pups were examined 2 months after their birth.The styrene exposure caused hearing loss and hair cell loss starting from the mid-frequency region in the third row of outer hair cells (OHCs) and the impairments appeared to be related to the dosing level in each single day.Significantly,the styrene exposure to the pregnant rats interfered with auditory functional development of their fetus,leading to a deficit of cochlear amplification,although the OHCs appeared to develop well.The results indicate that a short-term high-level styrene exposure may be more ototoxic than a long-term low-level exposure for a similar total styrene dose and the styrene in the pregnant woman’s body may interfere with auditory development of their fetus.
基金supported by the National Natural Science Foundation of China under Grant No.61202406the USTC Grand Master Professor Funds under Grant No.ZC9850290097
文摘With promising applications in e-health and entertainment, wireless body area networks (WBANs) have attracted the interest of both academia and industry. If WBANs are densely deployed within a small area, serious problems may arise between the WBANs. In this paper, we discuss issues related to the coexistence of WBANs and investigate the main factors that cause inter-WBAN interference. We survey inter- WBAN interference mitigation strategies and track recent research developments. We also discuss unresolved issues related to inter-WBAN interference mitigation and propose fu- ture research directions.
基金supported by the National Science Foundation(ECCS-1807463 and PFI-1718177)UB Blue Sky program+2 种基金funding support from National Cancer Institute(NCI)of the National Institutes of Health(NIH)(R21CA235305)funded by NCI(P30CA16056)the support from National Science Foundation(CBET-1337860),which funds the nanoparticle tracking analysis system(Nano Sight,LM10,Malvern Instruments,Ltd.)。
文摘Subwavelength manipulation of light waves with high precision can enable new and exciting applications in spectroscopy,sensing,and medical imaging.For these applications,miniaturized spectrometers are desirable to enable the on-chip analysis of spectral information.In particular,for imaging-based spectroscopic sensing mechanisms,the key challenge is to determine the spatial-shift information accurately(i.e.,the spatial displacement introduced by wavelength shift or biological or chemical surface binding),which is similar to the challenge presented by super-resolution imaging.Here,we report a unique"rainbow"trapping metasurface for on-chip spectrometers and sensors.Combined with super-resolution image processing,the low-setting 4×optical microscope system resolves a displacement of the resonant position within 35 nm on the plasmonic rainbow trapping metasurface with a tiny area as small as0.002 mm2.This unique feature of the spatial manipulation of efficiently coupled rainbow plasmonic resonances reveals a new platform for miniaturized on-chip spectroscopic analysis with a spectral resolution of 0.032 nm in wavelength shift.Using this low-setting 4×microscope imaging system,we demonstrate a biosensing resolution of 1.92×109exosomes per milliliter for A549-derived exosomes and distinguish between patient samples and healthy controls using exosomal epidermal growth factor receptor(EGFR)expression values,thereby demonstrating a new on-chip sensing system for personalized accurate bio/chemical sensing applications.
基金supported by the National Natural Science Foundation of China(NSFC)[grant numbers 41501383,91438203]China Postdoctoral Science Foundation[grant number 2014M562006]+1 种基金Natural Science Foundation of Hubei Province[grant number 2015CFB330]Fundamental Research Funds for the Central Universities[grant number 2042016kf0163].
文摘The development of global informatization and its integration with industrialization symbolizes that human society has entered into the big data era.This article covers seven new characteristics of Geomatics(i.e.ubiquitous sensor,multi-dimensional dynamics,integration via networking,full automation in real time,from sensing to recognition,crowdsourcing and volunteered geographic information,and serviceoriented science),and puts forward the corresponding critical technical challenges in the construction of integrated space-air-ground geospatial networks.Through the discussions outlined in this paper,we propose a new development stage of Geomatics entitled‘Connected Geomatics,’which is defined as a multi-disciplinary science and technology that uses systematic approaches and integrates methods of spatio-temporal data acquisition,information extraction,network management,knowledge discovery,and spatial sensing and recognition,as well as intelligent location-based services pertaining to any physical objects and human activities on the earth.It is envisioned that the advancement of Geomatics will make a great contribution to human sustainable development.
基金National Science Foundation(NSF)(DMR-1506884)Army Research Office(ARO)(W911NF-15-1-0152)
文摘Recent experiments demonstrated that chiral symmetry breaking at an exceptional point(EP) is a viable route to achieve unidirectional laser emission in microring lasers. By a detailed semiconductor laser rate equation model,we show here that unidirectional laser emission at an EP is a robust regime. Slight deviations from the EP condition can break preferential unidirectional lasing near threshold via a Hopf instability. However, abovea "second" laser threshold, unidirectional emission is restored.