Nanoscale optical resolution with a large field of view is a critical feature for many research and industry areas,such as semiconductor fabrication,biomedical imaging,and nanoscale material identification.Several sca...Nanoscale optical resolution with a large field of view is a critical feature for many research and industry areas,such as semiconductor fabrication,biomedical imaging,and nanoscale material identification.Several scanning microscopes have been developed to resolve the inverse relationship between the resolution and field of view;however,those scanning microscopes still rely upon fluorescence labeling and complex optical systems.To overcome these limitations,we developed a dual-camera acoustofluidic nanoscope with a seamless image merging algorithm(alphablending process).This design allows us to precisely image both the sample and the microspheres simultaneously and accurately track the particle path and location.Therefore,the number of images required to capture the entire field of view(200×200μm)by using our acoustofluidic scanning nanoscope is reduced by 55-fold compared with previous designs.Moreover,the image quality is also greatly improved by applying an alpha-blending imaging technique,which is critical for accurately depicting and identifying nanoscale objects or processes.This dual-camera acoustofluidic nanoscope paves the way for enhanced nanoimaging with high resolution and a large field of view.展开更多
基金the National Institutes of Health(R01GM143439,R01HD103727,UH3TR002978,U18TR003778,R01GM132603,R01GM141055,R01GM135486,R44HL140800,and R44AG063643)the National Science Foundation(CMMI-2104295),and a National Science Foundation Graduate Research Fellowship under Grant No.1644868.
文摘Nanoscale optical resolution with a large field of view is a critical feature for many research and industry areas,such as semiconductor fabrication,biomedical imaging,and nanoscale material identification.Several scanning microscopes have been developed to resolve the inverse relationship between the resolution and field of view;however,those scanning microscopes still rely upon fluorescence labeling and complex optical systems.To overcome these limitations,we developed a dual-camera acoustofluidic nanoscope with a seamless image merging algorithm(alphablending process).This design allows us to precisely image both the sample and the microspheres simultaneously and accurately track the particle path and location.Therefore,the number of images required to capture the entire field of view(200×200μm)by using our acoustofluidic scanning nanoscope is reduced by 55-fold compared with previous designs.Moreover,the image quality is also greatly improved by applying an alpha-blending imaging technique,which is critical for accurately depicting and identifying nanoscale objects or processes.This dual-camera acoustofluidic nanoscope paves the way for enhanced nanoimaging with high resolution and a large field of view.
