The division of aqueous samples into microdroplet arrays has many applications in biochemical and medical analysis.Inspired by biological features,we propose a method to produce picoliter droplet arrays for single-cel...The division of aqueous samples into microdroplet arrays has many applications in biochemical and medical analysis.Inspired by biological features,we propose a method to produce picoliter droplet arrays for single-cell analysis based on physical structure and interface.A 0.9 pL droplet array with an RSD(relative standard deviation)less than 6.3%and a density of 49,000 droplets/cm^(2) was successfully generated on a PDMS chip(polydimethylsiloxane)from a micromachined glass mold.The droplet generation principle of the wetting behavior in the microholes with splayed sidewalls on the PDMS chip by liquid smearing was exploited.The feasibility of the picoliter droplets for bacterial single-cell analysis was verified by the separation of mixed bacteria into single droplets and isolated in situ bacteria propagation.展开更多
基金This work was supported by the National Science Foundation of China with Grant No.61874033 and 61674043the Natural Science Foundation of Shanghai Municipal with Grant No.18ZR1402600the State Key Laboratory of ASIC and System,Fudan University with Grant No.2018MS003.
文摘The division of aqueous samples into microdroplet arrays has many applications in biochemical and medical analysis.Inspired by biological features,we propose a method to produce picoliter droplet arrays for single-cell analysis based on physical structure and interface.A 0.9 pL droplet array with an RSD(relative standard deviation)less than 6.3%and a density of 49,000 droplets/cm^(2) was successfully generated on a PDMS chip(polydimethylsiloxane)from a micromachined glass mold.The droplet generation principle of the wetting behavior in the microholes with splayed sidewalls on the PDMS chip by liquid smearing was exploited.The feasibility of the picoliter droplets for bacterial single-cell analysis was verified by the separation of mixed bacteria into single droplets and isolated in situ bacteria propagation.
