A reliable and accurate liquid chromatography with evaporative light scattering detection(LC-ELSD)method was developed for simultaneous determination of five constituents in Xue-Fu-Zhu-Yu capsule,a widely used prescri...A reliable and accurate liquid chromatography with evaporative light scattering detection(LC-ELSD)method was developed for simultaneous determination of five constituents in Xue-Fu-Zhu-Yu capsule,a widely used prescription of traditional Chinese medicine(TCM).The chromatographic separation was performed on a Symmetry C_(18)column(150 mm×4.6 mm,5.0μm)using gradient elution of acetonitrile and water(containing 1.0%acetic acid).The flow rate was 0.6 mL/min and the temperature of column was 30℃.A good linearity correlation was obtained over the investigated concentration ranges.Recoveries of the five components varied from 97.31%to 99.84%(RSD<4.0%).The validated method was successfully applied to the quality assessment of Xue-Fu-Zhu-Yu capsule.展开更多
基金financially supported by the Scientific and Technological Innovation Project Foundation of Tianjin(No.06FZZDSH00404).
文摘A reliable and accurate liquid chromatography with evaporative light scattering detection(LC-ELSD)method was developed for simultaneous determination of five constituents in Xue-Fu-Zhu-Yu capsule,a widely used prescription of traditional Chinese medicine(TCM).The chromatographic separation was performed on a Symmetry C_(18)column(150 mm×4.6 mm,5.0μm)using gradient elution of acetonitrile and water(containing 1.0%acetic acid).The flow rate was 0.6 mL/min and the temperature of column was 30℃.A good linearity correlation was obtained over the investigated concentration ranges.Recoveries of the five components varied from 97.31%to 99.84%(RSD<4.0%).The validated method was successfully applied to the quality assessment of Xue-Fu-Zhu-Yu capsule.
