Objective： To observe the effects of electro-acupuncture on S-100 protein expression of nerve fibers on the regeneration process of transected sciatic nerve of rats, so as to explore the partial mechanism of electro-...Objective： To observe the effects of electro-acupuncture on S-100 protein expression of nerve fibers on the regeneration process of transected sciatic nerve of rats, so as to explore the partial mechanism of electro-acupuncture treatment for peripheral nerve injury. Methods： Following rat＇s sciatic nerve transection, the nerve regeneration chamber model was established, then the rat was given electro-acupuncture stimulation at the frequency of 5 Hz and frequency of 100 Hz, and the immunohistochemical staining method and image analyzing semi-quantitative technique were adopted to determine the percentage of S-100 protein area, hence to observethe effect of electro-acupuncture on the repair process of transected sciatic nerve of rat and the S-100 protein expression. Results： Electro-acupuncture could significantly potentiate the S-100 protein expression of Schwann Cells （SC） in the repair process of transected sciatic nerve. At the 14th week, 17th week and 20th week after operation, the percentages of S-100 protein area were more obviously increased in 5 Hz electro-acupuncture group and 100 Hz electro-acupuncture group than in model group, with significant differences （P〈0.05）. Especially, the better effect was obtained in 5 Hz electro-acupuncture group. Judging from the dynamic treatment, it is very vital to be treated early. Conclusion： Electro-acupuncture is an effective method for the treatment of peripheral nerve injury, and its therapeutic effect is related to the improvement of SC proliferation.展开更多
文摘Objective： To observe the effects of electro-acupuncture on S-100 protein expression of nerve fibers on the regeneration process of transected sciatic nerve of rats, so as to explore the partial mechanism of electro-acupuncture treatment for peripheral nerve injury. Methods： Following rat＇s sciatic nerve transection, the nerve regeneration chamber model was established, then the rat was given electro-acupuncture stimulation at the frequency of 5 Hz and frequency of 100 Hz, and the immunohistochemical staining method and image analyzing semi-quantitative technique were adopted to determine the percentage of S-100 protein area, hence to observethe effect of electro-acupuncture on the repair process of transected sciatic nerve of rat and the S-100 protein expression. Results： Electro-acupuncture could significantly potentiate the S-100 protein expression of Schwann Cells （SC） in the repair process of transected sciatic nerve. At the 14th week, 17th week and 20th week after operation, the percentages of S-100 protein area were more obviously increased in 5 Hz electro-acupuncture group and 100 Hz electro-acupuncture group than in model group, with significant differences （P〈0.05）. Especially, the better effect was obtained in 5 Hz electro-acupuncture group. Judging from the dynamic treatment, it is very vital to be treated early. Conclusion： Electro-acupuncture is an effective method for the treatment of peripheral nerve injury, and its therapeutic effect is related to the improvement of SC proliferation.
