OBJECTIVE:To investigate the efficacy of phospholipid complex of flavonoids from persimmon leaves(PLF-PC)on atherosclerosis,and to study its mechanism behind the action.METHODS:To clarify the constituents of the flavo...OBJECTIVE:To investigate the efficacy of phospholipid complex of flavonoids from persimmon leaves(PLF-PC)on atherosclerosis,and to study its mechanism behind the action.METHODS:To clarify the constituents of the flavonoids from persimmon leaves(PLF),an ultra-performance liquid chromatography/quadrupole-time-of-flight mass spectrometry method was established.To enhance the anti-atherosclerotic effect of PLF,a newly emerging approach based on the combination of phospholipid complexation technique was employed.PLF-PC was prepared by the solvent-evaporation method then characterized using Fourier transform infrared spectroscopy,Powder X-Ray Diffractometry and Scanning electron microscopy.A model of oxidized lowdensity lipoprotein-induced injury on human umbilical vein endothelial cells was established to investigate the anti-atherosclerotic effect of PLF-PC versus PLF.The levels of nitric oxide,endothelial nitric oxide synthase,intracellular adhesion molecule-1,reactive oxygen species,superoxide dismutase,tumor necrosis factor-αand nuclear factor-κB were observed via assay kits.RESULTS:A total of 31 compounds were identified in PLF.PLF-PC showed better anti-atherosclerotic power compared with PLF,moreover,enzyme linked immuneosorbent assay analysis showed that the PLF-PC may effect on endothelial dysfunction and atherosclerosis via antioxidant-related mechanisms.CONCLUSIONS:Our findings elucidated that PLF-PC significantly enhanced the PLF’s efficacy on atherosclerosis.展开更多
基金Supported by National Science and Technology Major Project of China(Research on Evaluation Technology of New Traditional Chinese Medicine Based on Big Data of Classical Prescription,No.2019ZX09201005)International Cooperation Project of Traditional Chinese Medicine(China-Germany International Cooperation in Innovative Research and Development of Traditional Chinese Medicine and Botanical Medicine,No.0610-2140NF020630)
文摘OBJECTIVE:To investigate the efficacy of phospholipid complex of flavonoids from persimmon leaves(PLF-PC)on atherosclerosis,and to study its mechanism behind the action.METHODS:To clarify the constituents of the flavonoids from persimmon leaves(PLF),an ultra-performance liquid chromatography/quadrupole-time-of-flight mass spectrometry method was established.To enhance the anti-atherosclerotic effect of PLF,a newly emerging approach based on the combination of phospholipid complexation technique was employed.PLF-PC was prepared by the solvent-evaporation method then characterized using Fourier transform infrared spectroscopy,Powder X-Ray Diffractometry and Scanning electron microscopy.A model of oxidized lowdensity lipoprotein-induced injury on human umbilical vein endothelial cells was established to investigate the anti-atherosclerotic effect of PLF-PC versus PLF.The levels of nitric oxide,endothelial nitric oxide synthase,intracellular adhesion molecule-1,reactive oxygen species,superoxide dismutase,tumor necrosis factor-αand nuclear factor-κB were observed via assay kits.RESULTS:A total of 31 compounds were identified in PLF.PLF-PC showed better anti-atherosclerotic power compared with PLF,moreover,enzyme linked immuneosorbent assay analysis showed that the PLF-PC may effect on endothelial dysfunction and atherosclerosis via antioxidant-related mechanisms.CONCLUSIONS:Our findings elucidated that PLF-PC significantly enhanced the PLF’s efficacy on atherosclerosis.
