Objective: To identify the in vivo metabolites of icaritin and speculate its metabolic profiling in rats.Methods: The plasma, bile, urine, and feces of rats were collected after orally administration of icaritin at a ...Objective: To identify the in vivo metabolites of icaritin and speculate its metabolic profiling in rats.Methods: The plasma, bile, urine, and feces of rats were collected after orally administration of icaritin at a dose of 100 mg/kg and detected by an ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry(UPLC/Q-TOF-MS/MS) in both positive and negative modes.The data of treated and control groups were compared and analyzed with the aid of Metabolynx XS software.Results: A total of 25 metabolites were identified in the biosamples, and 14 of them were reported for the first time to our knowledge.Conclusion: The main metabolite types of icaritin in rats were glucuronide conjugation, methylation, hydroxylation, reduction, and acetylation.展开更多
Angelicae Sinensis Radix (ASR) is the root of Angelica sinensis which is a fragrant and perennial herb native to China, Japan, and Korea. In traditional Chinese medicine (TCM), the plant is useful for replenishing and...Angelicae Sinensis Radix (ASR) is the root of Angelica sinensis which is a fragrant and perennial herb native to China, Japan, and Korea. In traditional Chinese medicine (TCM), the plant is useful for replenishing and invigorating blood, relieving pain, and moistening the intestines, resulting in its application for the treatment of menstrual disorders, and as an emollient and laxative for chronic constipation of the aged and debilitated. An in-depth review of the literature brings to light a great number of chemical constituents that have been isolated from ASR as well as both preclinical (in vivo and in vitro) and clinical studies, which over the years, have sought to investigate the medicinal relevance of some of these phytoconstituents and/or extract(s) prepared from ASR. The purpose of this review is therefore to present some major pharmacological and pharmacokinetic research findings on some selected phytoconstituents of ASR with emphasis on the current trends in terms of research techniques or design. This review would also provide a wealth of information for users/practitioners of TCM regarding the use of ASR or its products for maximum efficiency and minimal toxicity or side effects.展开更多
基金supported by the National Natural Science Foundation of China (NSFC) [No. 81430095]
文摘Objective: To identify the in vivo metabolites of icaritin and speculate its metabolic profiling in rats.Methods: The plasma, bile, urine, and feces of rats were collected after orally administration of icaritin at a dose of 100 mg/kg and detected by an ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry(UPLC/Q-TOF-MS/MS) in both positive and negative modes.The data of treated and control groups were compared and analyzed with the aid of Metabolynx XS software.Results: A total of 25 metabolites were identified in the biosamples, and 14 of them were reported for the first time to our knowledge.Conclusion: The main metabolite types of icaritin in rats were glucuronide conjugation, methylation, hydroxylation, reduction, and acetylation.
基金State 973 Plan Project of China (2010CB735602)Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT, IRT0973)+1 种基金National Administration of Traditional Chinese Medicine of China (200807051)State Key Laboratory of Pharmaceutical New-tech for Chinese Medicine (SKL2010Z0302)
文摘Angelicae Sinensis Radix (ASR) is the root of Angelica sinensis which is a fragrant and perennial herb native to China, Japan, and Korea. In traditional Chinese medicine (TCM), the plant is useful for replenishing and invigorating blood, relieving pain, and moistening the intestines, resulting in its application for the treatment of menstrual disorders, and as an emollient and laxative for chronic constipation of the aged and debilitated. An in-depth review of the literature brings to light a great number of chemical constituents that have been isolated from ASR as well as both preclinical (in vivo and in vitro) and clinical studies, which over the years, have sought to investigate the medicinal relevance of some of these phytoconstituents and/or extract(s) prepared from ASR. The purpose of this review is therefore to present some major pharmacological and pharmacokinetic research findings on some selected phytoconstituents of ASR with emphasis on the current trends in terms of research techniques or design. This review would also provide a wealth of information for users/practitioners of TCM regarding the use of ASR or its products for maximum efficiency and minimal toxicity or side effects.
