MiR-25-3p attenuates the proliferation of tongue squamous cell carcinoma cell line Tca8113

Objective:To investigate the effects of miR-25-3p on the occurrence,development and proliferation of tongue squamous cell carcinoma cells.Methods:To establish tongue squamous cell carcinoma cell line Tca8113 that stably and highly express miR-25-3p using recombinant reiroviral vector-mediated gene transfer method.The proliferation of transfected Tca8113 was detected by thiazolyl blue tetrazolium bromide(MTT)and cell colony formation assays.eyclnD1,p21^(cipt)and p27^(kipt)mRNA expressions in the transfected Tca-8113 were detected by quantitative PCR.cyclinD1,p21^(cipt),p27^(kipt),AKT,p-AKT,FOXOt and p-FOX01 expressions in the transfected Tca8113 were detected by western blot analysis.In addition,miR-25-3p expression in the tongue squamous cell carcinoma cell line and tissue specimen was also detected by quantitative PCR.Results:Quantitative PCR showed that mitt-25-3p expression in the tongue squamous cell carcinoma cell lines and tissue specimen was significantly lower than that in the adjacent tissue.MTT and cell colony formation assays showed that after miR-25-3p overexpression,the proliferation of transfected Tca8113 was obviously attenuated.Western blot analysis and quantitative PCR showed that after miR-25-3p overexpression.p21^(cipt)and p27^(kipt)expressions were upregulated,while cyclinD1,AKT,FOXO1 expressions were downregulated,and AKT and FOXO1 phosphorylation was inactivated in the transfected Tca8113 cells.Conclusions:MiR-25-3p inhibited the proliferation of tongue squamous cell carcinoma cells and regulated cell cycle-related protein expression,playing an important role in the occurrence and development of squamous cell carcinoma of the tongue.

THE SINGULAR PERTURBATION METHOD APPLIED TO THE NONLINEAR STABILITY PROBLEM OF A SHALLOW SPHERICAL SHELL(Ⅱ)

In this paper we consider the nonlinear stability of a thin elastic circular shallow spherical shell under the action of uniform normal pressure with a clamped edge. When the geometrical parameter k is large, the uniformly valid asymptotic solutions are obtained by means of the singular perturbation method. In addition, we give the analytic formula for determining the centre deflection and the critical load, and the stability curve is also derived. This paper is a continuation of the author's previous paper[11]

The application of biomacromolecules to improve oral absorption by enhanced intestinal permeability:A mini-review

Oral administration has been widely regarded as the most conve nient,quick and safe approach compared to other routes of drug delivery.However,oral absorption of drugs is often limited due to rigorous environments and complex obstacles in gastrointestinal tract.Having received considerable attention,biomacromolecules have been applied for oral drug delivery to improve the bioavailability,which could be attributed to its stability and unique bioactivities,including intestinal adhesion,opening of epithelial tight junctions,inhibiting cell efflux and regulating relative protein expression.Specifically,enhancing intestinal permeability has been regarded as a promising strategy for improving bioavailability of oral drug delivery.In this review,a series of biomacromolecules and the related mechanisms of increasing intestinal permeability for enhanced oral bioavailability are comprehensively classified and elucidated.In addition,recent advances in biomacromolecules based oral delivery and related future directions are mentioned and predicted in this review article.

基于区域功能化石墨烯纤维的能量转化和储存集成器件

近年来,柔性化、微型化、集成化能量转换和储存器件引起人们的广泛关注,其中电极材料发挥着举足轻重的作用.一种电极材料通常只能满足一种特定能源器件的需求,如何通过电极材料的结构化设计,获得一种能同时满足两种或两种以上能源器件的共用电极,对于高性能集成器件具有重要意义.本文基于化学气相沉积法生长的连续石墨烯中空纤维,通过对管内选择性修饰,报道了一端生长聚苯胺、另一端生长铂纳米颗粒的局域功能化石墨烯纤维.其中,聚苯胺/石墨烯纤维可作为纤维状超级电容器的电极,器件的比容量达到284 m F cm-2;铂/石墨烯纤维部分可取代传统的铂丝对电极,用于染料敏化太阳能电池,能量转换效率为4.73%.以该区域功能化石墨烯纤维作为共用电极,所发展的太阳能电池/超级电容器集成器件的总能量转换和储存效率达到3.07%,明显高于其他已报道的纤维状能量转换和储存集成器件.另外,利用该方法能够进一步在连续中空石墨烯管内修饰其他活性材料,从而应用于其他柔性可穿戴电子器件领域.

Simultaneous inversion of layered compressional velocity and shear velocity by using plane wave seismogram

A layer-stripping method is presented for simultaneous inversion of compressional velocity and shear velocity in layered medium from single precritical-incident-angle data of P-P and P-SV plane wave seismogram. A finite bandwidth algorithm is provided and results obviously better than previous research work are obtained by the numerical experiments for band-limited seismogram and synthetic data including noise.

Morphology mediation of MoS_(2) nanosheets with organic cations for fast sodium ion storage

Ion diffusion kinetics,depending on the size,tortuosity,connectivity of the channels,greatly affects the rate performance of the electrodes.Two-dimensional materials(2DMs) has emerged as promising electrode materials in the past decades.Howeve r,the applications of 2DMs electrodes are limited by the strong restacking problem,which leads to a poor rate capability.In this work,we for the first time mediated the mo rphology of molybdenum disulfide(MoS_(2)) nanosheets via a facile coagulation method;abundant sheet crumples were induced,which greatly enhance their surface accessibility and thus benefit the ion diffusion kinetics.Consequently,the crumpled-MoS_(2) electrodes follow a capacitive Na-ion charge-storage mechanism to a large extent.Importantly,we demonstrate the special role of organic cations in the inter-sheet assembly configuration,in sharp contrast with that of alkali/alkaline-earth ones.We propose that organic cations cause edge/face contact of the sheets,instead of the face/face contact,thus affording a house-of-cards structure.

Amorphous silicon from low-temperature reduction of silica in the molten salts and its lithium-storage performance

Amorphous silicon(a-Si) is one of the most promising anode-materials for the lithium-ion battery owing to its large capacity and superior fracture resistance.However,a-Si is usually fabricated with the sophisticated chemical vapor deposition or pulse laser deposition in a limited scale.In this work,we have succes s fully pre pared a-Si spheres(~200 nm) by reducing the TiO2-coated silica spheres with Al powders in the molten salts at 300℃.The coated TiO2 layer acts as a protective layer for structural maintenance during the reduction and a precursor for doping.The doped Ti element may suppress the crystal growth of Si to facilitate the formation of a-Si.The observation with in-situ transmission electron microscopy(TEM) further reveals that lithiation kinetics of the synthesized a-Si is controlled by the interfacial reaction.The Li^(+) diffusivity in a-Si determined from the observation is in the order of 10^(-14) cm^(2)/s.The anode of a-Si spheres together with crystalline Si nanoparticles exhibits excellent electrochemical performance,delivering a reversible capacity of 1604 mAh/g at 4 A/g and a capacity retention of 78,3%after 500 cycles.The low temperature reduction process reported in this study provides a low-cost method to fabricate a-Si nanostructures as high-capacity durable anode materials.

Supercapacitors

Due to outstanding storage capacity,ultra-high power density,super long cycle life time,moderate energy density and high safety,supercapacitors have enthusiastically become a new type of energy storage device and energy storage technology after lithium ion battery.