The last couple of years have witnessed an explosion in development of CRISPR-based genome editing technologies in cell lines as well as in model organisms. In this review, we focus on the applications of this popular...The last couple of years have witnessed an explosion in development of CRISPR-based genome editing technologies in cell lines as well as in model organisms. In this review, we focus on the applications of this popular system in Drosophila. We discuss the effectiveness of the CRISPR/Cas9 systems in terms of delivery, mutagenesis detection, parameters affecting efficiency, and off-target issues, with an emphasis on how to apply this powerful tool to characterize gene functions.展开更多
基金supported by the National Key Technology Research and Development Program of the Ministry of Science and Technology of the People’s Republic of China(No.2015BAI09B03)the National Basic Research Program(973 Program)(No.2013CB35102)+3 种基金the National Natural Science Foundation of China(Nos.31371496 and31301008)the Natural Science Foundation of Hubei Province(No.2013CFB031)Wuhan Youth Chenguang Program of Science and Technology(No.2014072704011259)Tsinghua-Peking Center for Life Sciences
文摘The last couple of years have witnessed an explosion in development of CRISPR-based genome editing technologies in cell lines as well as in model organisms. In this review, we focus on the applications of this popular system in Drosophila. We discuss the effectiveness of the CRISPR/Cas9 systems in terms of delivery, mutagenesis detection, parameters affecting efficiency, and off-target issues, with an emphasis on how to apply this powerful tool to characterize gene functions.
