Objective To observe the expression of inlfammatory molecules in bone marrow immune cells of patients with immune-related hematocytopenia (IRH), and to investigate the immune mechanism and clinical signiifcance of the...Objective To observe the expression of inlfammatory molecules in bone marrow immune cells of patients with immune-related hematocytopenia (IRH), and to investigate the immune mechanism and clinical signiifcance of the disease. Methods Total of 36 IRH patients were selected as observation group and 30 healthy people were taken as control group. Serum cytokines levels, activity of immunocytes and expression of HLA-DR were detected. Immune lfuorescence was applied to observe the expression state of immunologic molecules and cytokines in IRH patients. Results Serum cytokines were elevated in various degrees in observation group. Compared with the control group, the cytokines levels were significantly higher (P < 0.05). After treatement with immunosuppressive drugs, the serum levels of cytokines in observation group reduced to a level close to the control group. HLA-DR were upregulated in activated tissue basophils, eosinophils, dendritic cells (DC) and macrophages of bone marrow in IRH patients, and POX activity in these immunocytes of IRH was higher than that of the control group. Immune molecules were highly expressed in eosinophils, DC and macrophages. Conclusions It is demonstrated that antibodies or self-reactive lymphocytes were produced in IRH marrow, which would cause lesions of hemocytes, and lead to pathological process ifnally. Structure of hematopoietic cells mutated and these cells might be acted as target cells of immunocytes in the pathological process. Immunocytes could secrete inlfammatory factors and lead to immunologic injury of hemocyte.展开更多
Objective： To investigate the effect of point injection on post-chemotherapeutic leukopenia. Methods： Three hundred patients were randomly divided into two groups. In the treatment group, 0.6 ml granulocyte colony s...Objective： To investigate the effect of point injection on post-chemotherapeutic leukopenia. Methods： Three hundred patients were randomly divided into two groups. In the treatment group, 0.6 ml granulocyte colony stimulating factor was injected into bilateral Xuehai （SP 10） and in the control group, the same dose subcutaneously into the deltoid muscle in the upper arm, once daily, three consecutive times as a course of treatment. A peripheral leukocyte count was examined at 3rd, 5th and 7th day after injection. Results and Conclusion： Under three courses of chemotherapy, therewas no significant difference in leukocyte increase between the two methods at 3rd and 5th day after treatment but a leukocyte count was significantly higher at 7th day in the treatment group than in the control group （P〈0.05）. After over half courses of chemotherapy, a peripheral leukocyte count was significantly higher in the treatment group than in the control group （P〈0.05） and it did not rise to the normal at 5-7 days after treatment in the control group. It is showed that point injection raised leukocyte quickly and kept the effect long in comparison with subcutaneous injection and the adverse responses were significantly less in the treatment group than in the control group.展开更多
文摘Objective To observe the expression of inlfammatory molecules in bone marrow immune cells of patients with immune-related hematocytopenia (IRH), and to investigate the immune mechanism and clinical signiifcance of the disease. Methods Total of 36 IRH patients were selected as observation group and 30 healthy people were taken as control group. Serum cytokines levels, activity of immunocytes and expression of HLA-DR were detected. Immune lfuorescence was applied to observe the expression state of immunologic molecules and cytokines in IRH patients. Results Serum cytokines were elevated in various degrees in observation group. Compared with the control group, the cytokines levels were significantly higher (P < 0.05). After treatement with immunosuppressive drugs, the serum levels of cytokines in observation group reduced to a level close to the control group. HLA-DR were upregulated in activated tissue basophils, eosinophils, dendritic cells (DC) and macrophages of bone marrow in IRH patients, and POX activity in these immunocytes of IRH was higher than that of the control group. Immune molecules were highly expressed in eosinophils, DC and macrophages. Conclusions It is demonstrated that antibodies or self-reactive lymphocytes were produced in IRH marrow, which would cause lesions of hemocytes, and lead to pathological process ifnally. Structure of hematopoietic cells mutated and these cells might be acted as target cells of immunocytes in the pathological process. Immunocytes could secrete inlfammatory factors and lead to immunologic injury of hemocyte.
文摘Objective： To investigate the effect of point injection on post-chemotherapeutic leukopenia. Methods： Three hundred patients were randomly divided into two groups. In the treatment group, 0.6 ml granulocyte colony stimulating factor was injected into bilateral Xuehai （SP 10） and in the control group, the same dose subcutaneously into the deltoid muscle in the upper arm, once daily, three consecutive times as a course of treatment. A peripheral leukocyte count was examined at 3rd, 5th and 7th day after injection. Results and Conclusion： Under three courses of chemotherapy, therewas no significant difference in leukocyte increase between the two methods at 3rd and 5th day after treatment but a leukocyte count was significantly higher at 7th day in the treatment group than in the control group （P〈0.05）. After over half courses of chemotherapy, a peripheral leukocyte count was significantly higher in the treatment group than in the control group （P〈0.05） and it did not rise to the normal at 5-7 days after treatment in the control group. It is showed that point injection raised leukocyte quickly and kept the effect long in comparison with subcutaneous injection and the adverse responses were significantly less in the treatment group than in the control group.
