Based on a combination of morphology and molecular data of ribosomal DNA genes,a new diatom genus Lineaperpetua gen.nov.Yu,You,Kociolek&Wang is described.The features that help define Lineaperpetua at the level of...Based on a combination of morphology and molecular data of ribosomal DNA genes,a new diatom genus Lineaperpetua gen.nov.Yu,You,Kociolek&Wang is described.The features that help define Lineaperpetua at the level of genus include:a tangentially undulated valve face;continuous cribra areolae on the valve interior consisting of pores arranged as strips;single rimoportula located inside the ring of marginal fultoportulae.Additionally,phylogenetic analysis based on nuclear small subunit(SSU)rDNA sequences and nuclear large subunit(LSU)rDNA gene placed the three strains of L.lacustris in a single,monophyletic clade at a considerable sequence distance from the other genera(Thalassiosira,Conticribra,Planktoniella,Shinodiscus,and other genera)belonging to Thalassiosirales.Despite the similarities with some species of Thalassiosira,Conticribra,and Spicaticribra,the suite of features found in Lineaperpetua differentiate it from these other genera.These molecular data and morphological characters suggest an affinity of the new genus to the Thalassiosiraceae.展开更多
Monitoring of host cell proteins(HCPs)during the manufacturing of monoclonal antibodies(mAb)has become a critical requirement to provide effective and safe drug products.Enzyme-linked immunosorbent assays are still th...Monitoring of host cell proteins(HCPs)during the manufacturing of monoclonal antibodies(mAb)has become a critical requirement to provide effective and safe drug products.Enzyme-linked immunosorbent assays are still the gold standard methods for the quantification of protein impurities.However,this technique has several limitations and does,among others,not enable the precise identification of proteins.In this context,mass spectrometry(MS)became an alternative and orthogonal method that delivers qualitative and quantitative information on all identified HCPs.However,in order to be routinely implemented in biopharmaceutical companies,liquid chromatography-MS based methods still need to be standardized to provide highest sensitivity and robust and accurate quantification.Here,we present a promising MS-based analytical workflow coupling the use of an innovative quantification standard,the HCP Profiler solution,with a spectral library-based data-independent acquisition(DIA)method and strict data validation criteria.The performances of the HCP Profiler solution were compared to more conventional standard protein spikes and the DIA approach was benchmarked against a classical datadependent acquisition on a series of samples produced at various stages of the manufacturing process.While we also explored spectral library-free DIA interpretation,the spectral library-based approach still showed highest accuracy and reproducibility(coefficients of variation<10%)with a sensitivity down to the sub-ng/mg mAb level.Thus,this workflow is today mature to be used as a robust and straightforward method to support mAb manufacturing process developments and drug products quality control.展开更多
Background: Trauma is major public health problem in developing countries where they are associated with many factors, mainly such as lack of road safety, poverty and inadequate urban planning. We aim to highlight the...Background: Trauma is major public health problem in developing countries where they are associated with many factors, mainly such as lack of road safety, poverty and inadequate urban planning. We aim to highlight the epidemiology of the fractures limbs bones in a rural area where people live in a context of low economy, low education and insecurity. Methods: We conducted a cross-sectional, retrospective study involving all patients admitted for fracture in the surgical department of FOMULAC-KATANA General Reference Hospital from 1<sup>st</sup> January 2011 to 31<sup>st</sup> December 2014. The patients whose medical records were not completed were excluded. Results: Among 136 patients included in our study, 140 bones limbs were registered. Most of them (60.1%) were aged between 15 - 44 years and the average age is 30, 1 year. Male predominance is noticed (72.8%) with a male/female ratio of 2.7/1, and informal professionals are the most found in our patients (67.6%). The main circumstances of fracture occurrence are, respectively, road traffic accidents (57.4%), falls (22.3%) and firearms (10.3%). The leg bones (33.6%) and forearm bones (20%) are the most concerned bones. Open fractures are recorded in 40.7% of patients. The supporting treatment is mainly orthopedic (64.5%) but also surgical (35.5). The mortality rate is 3.6%. Conclusion: The fractures of the limbs bones in Katana General Reference Hospital mainly affect young male which is informal professional. The road traffic accidents are the first circumstance leading to fractures followed by falls. The leg bones are the most fractured body part and fractures are commonly opened.展开更多
Universities collect and generate a considerable amount of data on students throughout their academic career. Currently in South Kivu, most universities have an information system in the form of a database made up of ...Universities collect and generate a considerable amount of data on students throughout their academic career. Currently in South Kivu, most universities have an information system in the form of a database made up of several disparate files. This makes it difficult to use this data efficiently and profitably. The aim of this study is to develop this transactional database-based information system into a data warehouse-oriented system. This tool will be able to collect, organize and archive data on the student’s career path, year after year, and transform it for analysis purposes. In the age of Big Data, a number of artificial intelligence techniques have been developed, making it possible to extract useful information from large databases. This extracted information is of paramount importance in decision-making. By way of example, the information extracted by these techniques can be used to predict which stream a student should choose when applying to university. In order to develop our contribution, we analyzed the IT information systems used in the various universities and applied the bottom-up method to design our data warehouse model. We used the relational model to design the data warehouse.展开更多
The contents and distributions of metal elements in the brain are closely related to neurodegenerative diseases.In this study, we examined Fe, Cu and Zn contents in the brain section associated with Parkinson‘s disea...The contents and distributions of metal elements in the brain are closely related to neurodegenerative diseases.In this study, we examined Fe, Cu and Zn contents in the brain section associated with Parkinson‘s disease(PD)using synchrotron radiation X-ray fluorescence(SRXRF). PD mouse model induced by 1-methyl-4-phenyl-1,2,3,6-terahydropyridine(MPTP) was used for the elemental analysis(e.g., Fe, Cu and Zn) in the substantia nigra pars compacta(SNpc) region of mice brain tissue samples. We found that mice in the MPTP group had higher contents of Fe, Cu and Zn in the SNpc than the control group. After treating the PD mice with rapamycin, the contents of Fe, Cu and Zn were reduced, the dopamine neurons and motor function were rescued correspondingly. The results prompted that the SRXRF provided an ideal method for tracing and analyzing the metal elements in the brain section to assess the pathological changes of PD model and the therapeutic effect of drugs.展开更多
Based on the research of a biological olfactory system, a novel chaotic neural network model - K set model has been es- tablished. This chaotic neural network not only simulates the real brain activity of an olfactor...Based on the research of a biological olfactory system, a novel chaotic neural network model - K set model has been es- tablished. This chaotic neural network not only simulates the real brain activity of an olfactory system, but also presents a novel chaotic concept for signal processing and pattern recognition. The characteristics of the K set models are investigated and show that a KIII model can be used for image pattern classification.展开更多
Significant progress has been made in understanding pharmacokinetics (PK),pharmacodynamics (PD),as well as toxicity profiles of therapeutic proteins in animals and humans,which have been in commercial development for ...Significant progress has been made in understanding pharmacokinetics (PK),pharmacodynamics (PD),as well as toxicity profiles of therapeutic proteins in animals and humans,which have been in commercial development for more than three decades.However,in the PK arena,many fundamental questions remain to be resolved.Investigative and bioanalytical tools need to be established to improve the translation of PK data from animals to humans,and from in vitro assays to in vivo readouts,which would ultimately lead to a higher success rate in drug development.In toxicology,it is known,in general,what studies are needed to safely develop therapeutic proteins,and what studies do not provide relevant information.One of the major complicating factors in nonclinical and clinical programs for therapeutic proteins is the impact of immunogenicity.In this review,we will highlight the emerging science and technology,as well as the challenges around the pharmacokinetic-and safety-related issues in drug development of mAbs and other therapeutic proteins.展开更多
Toluene degradation performances were studied in a 10 L Two-Phase Partitioning Bioreactor(TPPB).The liquid phase consisted of a mixture of water and PDMS 50(Poly Di Methyl Siloxane,i.e.silicone oil,viscosity of 46 m P...Toluene degradation performances were studied in a 10 L Two-Phase Partitioning Bioreactor(TPPB).The liquid phase consisted of a mixture of water and PDMS 50(Poly Di Methyl Siloxane,i.e.silicone oil,viscosity of 46 m Pa·s) in the volume ratio of 75%/25%.Two series of experiments were carried out:in the first,the reactor was sequentially supplied with toluene whereas in the second,toluene was continuously supplied.Activated sludge from the wastewater treatment plant of Beaurade(Rennes,France) was used at an initial concentration of 0.5 dry mass g·(mixture L)^(-1).The elimination capacity(EC) was investigated as well as the change in biomass concentration over time.Toluene biodegradation was very ef ficient(removal ef ficiency,RE=100%) for toluene flows ranging from 0.2 to 1.2 ml·h^(-1),corresponding to elimination capacities of up to 104 g·m^(-3)·h^(-1).For a toluene flow of 1.2 ml·h^(-1),the biomass concentration measured at the end of the experiment was 4.7 dry mass g·(mixture L)^(-1).The oxygen concentration in the liquid phase was clearly not a limiting factor in these operating conditions.Based on these results,an extrapolation leading to the design of a large-scale pilot TPPB can now be considered to study toluene degradation performances in industrial conditions.展开更多
In the summer of 2003 and 2004, characterized by a rapid glacier retreat, a stony surface covered by well-structured organic-rich mineral debris was observed very close to the Indren glacier terminus(Monte Rosa Massif...In the summer of 2003 and 2004, characterized by a rapid glacier retreat, a stony surface covered by well-structured organic-rich mineral debris was observed very close to the Indren glacier terminus(Monte Rosa Massif, NW Italy, 3100 m ASL), on an area covered by the glacier tongue till the year before. The origin and type of this organicrich material were investigated, in order to detect their characteristics, potential sources and fate within the foreland system. The deposits were dated using Carbon-14 and analyzed for the chemical characteristics of the organic component, the elemental composition of the mineral fraction and presence of microbial markers. The material, granular and dark in color, had a total organic carbon(TOC) content ranging between 17.4 ± 0.39 and 28.1 ± 0.63 g kg^(-1) dry weight(dw), significantly higher than the surrounding glacial till(~ 1.4 g kg^(-1) dw), although only 0.33% of it was in water soluble form. Microbial carbon(C) and nitrogen(N) accounted for 10.6% and 3.13% of TOC and total N, respectively. Dissolved nitrogen(N), mainly present as ammonium, represented 2.40% of the total N. The low aromatic component and large presence of nitrogen(N)-derived compounds suggested that most of the organic carbon(OC) in these organic-rich mineral deposits was derived from microbial cells, although the high average radiocarbon age of about 2900 years may also point to the contribution of aeolian depositions of anthropogenic or natural origin. Elemental composition and the crustal enrichment factor of trace elements in the mineral fraction of the aggregates corroborated the hypothesis that most part of the accumulated material derived from ice meltwater. Some indicators of the colonization of these deposits by microbial communities were also reported, from the abundance of DNA and phylogenetic markers, to the presence of bacterial taxa commonly able to thrive in similar habitats. All these elements suggested that such kind of deposits may have a potential role as energy and nutrient sources in recently deglaciated areas, highlighting the necessity to better understand the processes underlying their formation and their evolution.展开更多
In this work, we show that when there is insufficient equipment for detecting a disease whose prevalence is <em>t</em>% in a sub-population of size <em>N</em>, it is optimal to divide the <e...In this work, we show that when there is insufficient equipment for detecting a disease whose prevalence is <em>t</em>% in a sub-population of size <em>N</em>, it is optimal to divide the <em>N</em> samples into <em>n</em> groups of size r each and then, the value <img src="Edit_ce149849-3742-48fe-820b-02ccc0c92d83.bmp" alt="" /> allows systematic screening of all <em>N</em> individuals by performing less than <em>N</em> tests (In this expression, <img src="Edit_987eb236-a883-4894-ba2d-52bde5f35056.bmp" alt="" /> represents the floor function<sup>1</sup> of <em>x</em> ∈ R). Based on this result and on certain functions of the R software, we subsequently propose a probabilistic strategy capable of optimizing the screening tests under certain conditions.展开更多
We report on eight species of the freshwater diatom genus Gomphonema from aquatic habitats from the Yuntai Mountains of Henan Province,China.This work represents the first detailed descriptions of the freshwater diato...We report on eight species of the freshwater diatom genus Gomphonema from aquatic habitats from the Yuntai Mountains of Henan Province,China.This work represents the first detailed descriptions of the freshwater diatoms from this centrally-located province in China.Of the eight taxa considered,five are new to science and another is given a"cf."designation.Four of the eight species are similar in morphological features to other,"classical"species,with external areolar occlusions forming C-shaped openings.Another species is astigmate,and has distinct siliceous papillae located marginally on the exterior of the valves;this morphology has not been recognized previously for the genus.Two of the species have internal areolar and stigma occlusions.The diversity of morphological types of species in Gomphonema may relate to the ecosystems,with more diverse ecosystems harboring greater diversity of these morphological groups of Gomphonema.展开更多
基金the Postdoctoral Science Foundation of China(No.2021 M 703434)the National Natural Science Foundation of China(Nos.32100165,32170205)the Natural Science Foundation of Shanghai(No.21 ZR 144730)。
文摘Based on a combination of morphology and molecular data of ribosomal DNA genes,a new diatom genus Lineaperpetua gen.nov.Yu,You,Kociolek&Wang is described.The features that help define Lineaperpetua at the level of genus include:a tangentially undulated valve face;continuous cribra areolae on the valve interior consisting of pores arranged as strips;single rimoportula located inside the ring of marginal fultoportulae.Additionally,phylogenetic analysis based on nuclear small subunit(SSU)rDNA sequences and nuclear large subunit(LSU)rDNA gene placed the three strains of L.lacustris in a single,monophyletic clade at a considerable sequence distance from the other genera(Thalassiosira,Conticribra,Planktoniella,Shinodiscus,and other genera)belonging to Thalassiosirales.Despite the similarities with some species of Thalassiosira,Conticribra,and Spicaticribra,the suite of features found in Lineaperpetua differentiate it from these other genera.These molecular data and morphological characters suggest an affinity of the new genus to the Thalassiosiraceae.
基金supported by the“Association Nationale de la Recherche et de la Technologie”and UCB Pharma S.A.(Belgium and France)via the CIFRE fellowship of Steve Hessmannsupported by the“Agence Nationale de la Recherche”via the French Proteomic Infrastructure ProFI FR2048(ANR-10-INBS-08-03).
文摘Monitoring of host cell proteins(HCPs)during the manufacturing of monoclonal antibodies(mAb)has become a critical requirement to provide effective and safe drug products.Enzyme-linked immunosorbent assays are still the gold standard methods for the quantification of protein impurities.However,this technique has several limitations and does,among others,not enable the precise identification of proteins.In this context,mass spectrometry(MS)became an alternative and orthogonal method that delivers qualitative and quantitative information on all identified HCPs.However,in order to be routinely implemented in biopharmaceutical companies,liquid chromatography-MS based methods still need to be standardized to provide highest sensitivity and robust and accurate quantification.Here,we present a promising MS-based analytical workflow coupling the use of an innovative quantification standard,the HCP Profiler solution,with a spectral library-based data-independent acquisition(DIA)method and strict data validation criteria.The performances of the HCP Profiler solution were compared to more conventional standard protein spikes and the DIA approach was benchmarked against a classical datadependent acquisition on a series of samples produced at various stages of the manufacturing process.While we also explored spectral library-free DIA interpretation,the spectral library-based approach still showed highest accuracy and reproducibility(coefficients of variation<10%)with a sensitivity down to the sub-ng/mg mAb level.Thus,this workflow is today mature to be used as a robust and straightforward method to support mAb manufacturing process developments and drug products quality control.
文摘Background: Trauma is major public health problem in developing countries where they are associated with many factors, mainly such as lack of road safety, poverty and inadequate urban planning. We aim to highlight the epidemiology of the fractures limbs bones in a rural area where people live in a context of low economy, low education and insecurity. Methods: We conducted a cross-sectional, retrospective study involving all patients admitted for fracture in the surgical department of FOMULAC-KATANA General Reference Hospital from 1<sup>st</sup> January 2011 to 31<sup>st</sup> December 2014. The patients whose medical records were not completed were excluded. Results: Among 136 patients included in our study, 140 bones limbs were registered. Most of them (60.1%) were aged between 15 - 44 years and the average age is 30, 1 year. Male predominance is noticed (72.8%) with a male/female ratio of 2.7/1, and informal professionals are the most found in our patients (67.6%). The main circumstances of fracture occurrence are, respectively, road traffic accidents (57.4%), falls (22.3%) and firearms (10.3%). The leg bones (33.6%) and forearm bones (20%) are the most concerned bones. Open fractures are recorded in 40.7% of patients. The supporting treatment is mainly orthopedic (64.5%) but also surgical (35.5). The mortality rate is 3.6%. Conclusion: The fractures of the limbs bones in Katana General Reference Hospital mainly affect young male which is informal professional. The road traffic accidents are the first circumstance leading to fractures followed by falls. The leg bones are the most fractured body part and fractures are commonly opened.
文摘Universities collect and generate a considerable amount of data on students throughout their academic career. Currently in South Kivu, most universities have an information system in the form of a database made up of several disparate files. This makes it difficult to use this data efficiently and profitably. The aim of this study is to develop this transactional database-based information system into a data warehouse-oriented system. This tool will be able to collect, organize and archive data on the student’s career path, year after year, and transform it for analysis purposes. In the age of Big Data, a number of artificial intelligence techniques have been developed, making it possible to extract useful information from large databases. This extracted information is of paramount importance in decision-making. By way of example, the information extracted by these techniques can be used to predict which stream a student should choose when applying to university. In order to develop our contribution, we analyzed the IT information systems used in the various universities and applied the bottom-up method to design our data warehouse model. We used the relational model to design the data warehouse.
基金Supported by the Ministry of Science and Technology of China(Nos.2012CB825805 and 2012CB932600)National Natural Science Foundation of China(Nos.11179004,21390414,U1232113,U1232114,U1332119 and U1432116)Youth Innovation Promotion Association of CAS
文摘The contents and distributions of metal elements in the brain are closely related to neurodegenerative diseases.In this study, we examined Fe, Cu and Zn contents in the brain section associated with Parkinson‘s disease(PD)using synchrotron radiation X-ray fluorescence(SRXRF). PD mouse model induced by 1-methyl-4-phenyl-1,2,3,6-terahydropyridine(MPTP) was used for the elemental analysis(e.g., Fe, Cu and Zn) in the substantia nigra pars compacta(SNpc) region of mice brain tissue samples. We found that mice in the MPTP group had higher contents of Fe, Cu and Zn in the SNpc than the control group. After treating the PD mice with rapamycin, the contents of Fe, Cu and Zn were reduced, the dopamine neurons and motor function were rescued correspondingly. The results prompted that the SRXRF provided an ideal method for tracing and analyzing the metal elements in the brain section to assess the pathological changes of PD model and the therapeutic effect of drugs.
文摘Based on the research of a biological olfactory system, a novel chaotic neural network model - K set model has been es- tablished. This chaotic neural network not only simulates the real brain activity of an olfactory system, but also presents a novel chaotic concept for signal processing and pattern recognition. The characteristics of the K set models are investigated and show that a KIII model can be used for image pattern classification.
文摘Significant progress has been made in understanding pharmacokinetics (PK),pharmacodynamics (PD),as well as toxicity profiles of therapeutic proteins in animals and humans,which have been in commercial development for more than three decades.However,in the PK arena,many fundamental questions remain to be resolved.Investigative and bioanalytical tools need to be established to improve the translation of PK data from animals to humans,and from in vitro assays to in vivo readouts,which would ultimately lead to a higher success rate in drug development.In toxicology,it is known,in general,what studies are needed to safely develop therapeutic proteins,and what studies do not provide relevant information.One of the major complicating factors in nonclinical and clinical programs for therapeutic proteins is the impact of immunogenicity.In this review,we will highlight the emerging science and technology,as well as the challenges around the pharmacokinetic-and safety-related issues in drug development of mAbs and other therapeutic proteins.
基金the French Environment and Energy Management Agency(ADEME) for their support through a PhD fellowship for M.Guillerm
文摘Toluene degradation performances were studied in a 10 L Two-Phase Partitioning Bioreactor(TPPB).The liquid phase consisted of a mixture of water and PDMS 50(Poly Di Methyl Siloxane,i.e.silicone oil,viscosity of 46 m Pa·s) in the volume ratio of 75%/25%.Two series of experiments were carried out:in the first,the reactor was sequentially supplied with toluene whereas in the second,toluene was continuously supplied.Activated sludge from the wastewater treatment plant of Beaurade(Rennes,France) was used at an initial concentration of 0.5 dry mass g·(mixture L)^(-1).The elimination capacity(EC) was investigated as well as the change in biomass concentration over time.Toluene biodegradation was very ef ficient(removal ef ficiency,RE=100%) for toluene flows ranging from 0.2 to 1.2 ml·h^(-1),corresponding to elimination capacities of up to 104 g·m^(-3)·h^(-1).For a toluene flow of 1.2 ml·h^(-1),the biomass concentration measured at the end of the experiment was 4.7 dry mass g·(mixture L)^(-1).The oxygen concentration in the liquid phase was clearly not a limiting factor in these operating conditions.Based on these results,an extrapolation leading to the design of a large-scale pilot TPPB can now be considered to study toluene degradation performances in industrial conditions.
基金Manuscript writing was supported by the Italian MIUR Project(PRIN 2010–11):“Response of morphoclimatic system dynamics to global changes and related geomorphological hazards”(national coordinator C.Baroni)the contribution of MW Williams was supported by the US NSF-funded Niwot Ridge Long-Term Ecological Research program。
文摘In the summer of 2003 and 2004, characterized by a rapid glacier retreat, a stony surface covered by well-structured organic-rich mineral debris was observed very close to the Indren glacier terminus(Monte Rosa Massif, NW Italy, 3100 m ASL), on an area covered by the glacier tongue till the year before. The origin and type of this organicrich material were investigated, in order to detect their characteristics, potential sources and fate within the foreland system. The deposits were dated using Carbon-14 and analyzed for the chemical characteristics of the organic component, the elemental composition of the mineral fraction and presence of microbial markers. The material, granular and dark in color, had a total organic carbon(TOC) content ranging between 17.4 ± 0.39 and 28.1 ± 0.63 g kg^(-1) dry weight(dw), significantly higher than the surrounding glacial till(~ 1.4 g kg^(-1) dw), although only 0.33% of it was in water soluble form. Microbial carbon(C) and nitrogen(N) accounted for 10.6% and 3.13% of TOC and total N, respectively. Dissolved nitrogen(N), mainly present as ammonium, represented 2.40% of the total N. The low aromatic component and large presence of nitrogen(N)-derived compounds suggested that most of the organic carbon(OC) in these organic-rich mineral deposits was derived from microbial cells, although the high average radiocarbon age of about 2900 years may also point to the contribution of aeolian depositions of anthropogenic or natural origin. Elemental composition and the crustal enrichment factor of trace elements in the mineral fraction of the aggregates corroborated the hypothesis that most part of the accumulated material derived from ice meltwater. Some indicators of the colonization of these deposits by microbial communities were also reported, from the abundance of DNA and phylogenetic markers, to the presence of bacterial taxa commonly able to thrive in similar habitats. All these elements suggested that such kind of deposits may have a potential role as energy and nutrient sources in recently deglaciated areas, highlighting the necessity to better understand the processes underlying their formation and their evolution.
文摘In this work, we show that when there is insufficient equipment for detecting a disease whose prevalence is <em>t</em>% in a sub-population of size <em>N</em>, it is optimal to divide the <em>N</em> samples into <em>n</em> groups of size r each and then, the value <img src="Edit_ce149849-3742-48fe-820b-02ccc0c92d83.bmp" alt="" /> allows systematic screening of all <em>N</em> individuals by performing less than <em>N</em> tests (In this expression, <img src="Edit_987eb236-a883-4894-ba2d-52bde5f35056.bmp" alt="" /> represents the floor function<sup>1</sup> of <em>x</em> ∈ R). Based on this result and on certain functions of the R software, we subsequently propose a probabilistic strategy capable of optimizing the screening tests under certain conditions.
基金Supported by the National Natural Science Foundation of China(No.31970217)the One-hundred Talent Program of Shanxi Province and the Key Research and the Development(R&D)Projects of Shanxi Province(No.201903D421038)。
文摘We report on eight species of the freshwater diatom genus Gomphonema from aquatic habitats from the Yuntai Mountains of Henan Province,China.This work represents the first detailed descriptions of the freshwater diatoms from this centrally-located province in China.Of the eight taxa considered,five are new to science and another is given a"cf."designation.Four of the eight species are similar in morphological features to other,"classical"species,with external areolar occlusions forming C-shaped openings.Another species is astigmate,and has distinct siliceous papillae located marginally on the exterior of the valves;this morphology has not been recognized previously for the genus.Two of the species have internal areolar and stigma occlusions.The diversity of morphological types of species in Gomphonema may relate to the ecosystems,with more diverse ecosystems harboring greater diversity of these morphological groups of Gomphonema.