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Human pluripotent stem cells:From biology to cell therapy 被引量:2
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作者 Jean-Marie Ramirez Marilyne Dijon-Grinand +3 位作者 Said Assou Sabine Gerbal-Chaloin Samir Hamamah John De Vos 《World Journal of Stem Cells》 SCIE CAS 2010年第2期24-33,共10页
Human pluripotent stem cells(PSCs),encompassing embryonic stem cells and induced pluripotent stem cells,proliferate extensively and differentiate into virtually any desired cell type.PSCs endow regenerative medicine w... Human pluripotent stem cells(PSCs),encompassing embryonic stem cells and induced pluripotent stem cells,proliferate extensively and differentiate into virtually any desired cell type.PSCs endow regenerative medicine with an unlimited source of replacement cells suitable for human therapy.Several hurdles must be carefully addressed in PSC research before these theoretical possibilities are translated into clinical applications.These obstacles are:(1) cell proliferation;(2) cell differentiation;(3) genetic integrity;(4) allogenicity;and(5) ethical issues.We discuss these issues and underline the fact that the answers to these questions lie in a better understanding of the biology of PSCs.To contribute to this aim,we have developed a free online expression atlas,Amazonia!,that displays for each human gene a virtual northern blot for PSC samples and adult tissues(http://www.amazonia.transcriptome.eu). 展开更多
关键词 PLURIPOTENT STEM CELLS EMBRYONIC STEM CELLS Therapeutics CELL REPROGRAMMING CELL proliferation CELL differentiation
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WIRELESS DISTRIBUTED ACQUISITION SYSTEM FOR NEAR INFRARED SPECTROSCOPY-WDA-NIRS
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作者 J.SAFAIE R.GREBE +1 位作者 H.ABRISHAMI MOGHADDAM F.WALLOIS 《Journal of Innovative Optical Health Sciences》 SCIE EI CAS 2013年第3期31-45,共15页
The wireless distributed acquisition system for near infrared spectrosecopy(WDA-NIRS)is a portable,ultra-compact,contimuous wave(CW)NIRS system.Its main advantage is that it allows continuous synchronized multi-site h... The wireless distributed acquisition system for near infrared spectrosecopy(WDA-NIRS)is a portable,ultra-compact,contimuous wave(CW)NIRS system.Its main advantage is that it allows continuous synchronized multi-site hemodynarnic monitoring.The WDA-NIRS syster calculates online changes in hemoglobin concentration based on modifed Beer-Lambert law and the tissue oxy genation index based on the spatial resolved spectroscopy method.It consists of up to seven signal acquisition units,sufficiently small to be easily attached to any part of the body.These units are remotely synchronized by a PC base station for independent acquisition of NIRS signals.Each acquisition module can be freely adapted to individual requirements such as local skin properties and the microcirculation of interest,e.g,different muscles,brain,skin,etc.For this purpose,the light emitted by each LED can be individually,interactively or automatically adjusted to local needs.Furthermore,the user can freely create an emitter time multiplexing protocol and choose the detector sensitivity most suitable to a particular situation.The potential diagnostic value of this advanced device is demonstrated by three typical applications. 展开更多
关键词 NIRS optical spectroscopy body area network MICROCIRCULATION
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Sperm concentration measurement with a disposable counting chamber 被引量:1
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作者 Mathilde Lemoine Xavier Ferraretto +5 位作者 Marie-Astrid Llabador-de Royer Achraf Benammar Jacques Darolles Sylvie Epelboin Florence Eustache Catherine Patrat 《Asian Journal of Andrology》 SCIE CAS CSCD 2018年第5期525-526,共2页
Dear Editor, In its latest edition, the World Health Organization (WHO) provides guidelines regarding the standardization of techniques for the measurement of sperm concentrationI and recommends use of a 100 μm-de... Dear Editor, In its latest edition, the World Health Organization (WHO) provides guidelines regarding the standardization of techniques for the measurement of sperm concentrationI and recommends use of a 100 μm-deep hemocytometer. However, these instructions are not always followed correctly.2 Furthermore, although hemocytometers provide more accurate and consistent results, they present several drawbacks: the chamber must be properly assembled prior to its use so as to ensure that the counts are correct and it needs to be cleaned thoroughly to remove all traces of sperm, which risks breaking the glass coverslip. 展开更多
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