PCR-RFLP鉴别念珠菌、曲霉和隐球菌的探讨

为快速鉴别 3类医学上重要真菌———念珠菌、曲霉和隐球菌 ,应用聚合酶链反应 限制性片段长度多态(PCR RFLP)的分子生物学方法对白念珠菌、克柔念珠菌、季也蒙念珠菌、近平滑念珠菌、热带念珠菌、乳酒念珠菌、新生隐球菌格梯变种、新生隐球菌新生变种、罗伦隐球菌、烟曲霉、黄曲霉和杂色曲霉进行体外研究。经PCR念珠菌、曲霉和隐球菌均扩增出 3 10bp的DNA片段 ,再行限制性内切酶HaeIII消化后分别呈现 2条、4条和 3条片段。本实验建立的PCR RFLP技术鉴别念珠菌、曲霉和隐球菌感染 ,具有快速和特异的特点 ,是对PCR诊断的重要补充 ,有潜在的临床应用价值。

间质性输卵管息肉对输卵管峡直径的影响

Objective: To study the apparent association between interstitial fallopian tu be polyps and isthmic tubal dilatation. Design: Retrospective clinical study. Se tting: Tertiary academic medical center. Patient(s): Sixty-five patients with n ormal hysterosalpingograms and 40 patients with interstitial tubal polyps (25 un ilateral and 15 bilateral). Intervention(s): None. Main Outcome Measure(s): Prox imal, medial, and distal isthmic diameters were measured digitally and normalize d according to an internal standard. Polyp volumes were measured and divided int o four groups according to increasing volume (< 2 mm3,2-5 mm3,6-12 mm3, >12 mm 3). Result(s): There was a significant increase in proximal isthmic tubal diamet er in patients with interstitial polyps. Mean diameter increased from 0.6 mm to 0.9 mm. There was no significant change in more distal isthmic diameters. Larger polyps tended to be associated with larger luminal diameters. Conclusion(s): In terstitial fallopian tube polyps are associated with a significant increase in p roximal isthmic luminal diameter as determined on hysterosalpingography. Althoug h these polyps are nonobstructing and thus thought by most investigators not to affect fertility, their association with altered luminal diameter and perhaps al tered function might be of clinical interest.

玻璃珠-盐析法提取常见致病真菌DAN的研究

目的 进一步提高提取 DNA的效率并减少污染和毒性，以适应临床 PCR检测的需要，建立玻璃珠－盐析法快速提取常见致病真菌 DNA。方法 分别采用玻璃珠－盐析法和经典的酶解－酚氯仿法比较从白念珠菌、乳酒念珠菌、克柔念珠菌、季也蒙念珠菌、新生隐球菌、烟曲霉、黄曲霉和马内菲青霉等 8种常见致病真菌中提取 DNA，同时行 PCR以评价其可靠性。 结果 本方法从 8种常见致病真菌均提取浓度较高且较完整的 DNA，用于 PCR也获得良好结果。结论 玻璃珠－盐析法提取致病酵母菌和丝状真菌安全、快速且有效。