N-acetylcysteine inhibits activation of toll-like receptor 2 and 4 gene expression in the liver and lung after partial hepatic ischemia-reperfusion injury in mice

BACKGROUND: Toll-like receptor 2 and 4 (TLR2/4) may play important roles in ischemia-reperfusion (I/R) injury, and N-acetylcysteine (NAC) can prevent the generation of reactive oxygen species (ROS) induced by I/R injury. This study aimed to investigate the changes in TLR2/4 gene expression in the liver and lung after I/R injury with or without NAC pretreatment. METHODS: BALB/c mice were used in a model of partial hepatic I/R injury and randomly assigned to a sham-operated control group (SH), a hepatic ischemia/reperfusion group (I/R) or a NAC pretreated, hepatic I/R group (I/R-NAC). The levels of TNF-alpha in the portal vein and plasma alanine aminotransferase (ALT) were measured at 1 and 3 hours after reperfusion. The lung wet-to-dry ratio was measured, and the expression of TLR2/4 mRNA and protein in the liver and lung were assessed with RT-PCR and Western blotting at the same time points. RESULTS: Compared with the I/R group, the expression of TLR2/4 mRNA and protein in the liver and lung in the I/R-NAC group was decreased at the same time point (P<0.05). The levels of portal vein TNF-a and plasma ALT increased continuously in the l/R group at I and 3 hours of reperfusion compared with the SH group; however, they declined significantly in the group pretreated with NAC (P<0.05). The extent of lung edema was relieved in the I/R-NAC group compared with the I/R group (P<0.05). CONCLUSIONS: TLR2/4 was activated in the liver and lung in the process of partial hepatic I/R injury. NAC inhibited the activation of TLR2/4 and the induction of TNF-alpha resulting from I/R injury via modulating the redox state, thus it may mitigate liver and lung injury following partial hepatic I/R in mice.

Effect of miR-451-mediated regulation of MIF expression on cell proliferation in human colon carcinoma cell line LoVo

Objective To investigate the effect of miR-451-mediated regulation of macrophage migration inhibitory factor(MIF) expression on cell proliferation in human colon carcinoma cell line LoVo.Methods A lentiviral

Analysis on missed diagnosis or misdiagnosis of anomalous origin of left coronary artery from pulmonary artery by echocardiography from one single medical center

Objective To analyze the reasons of missed diagnosis or misdiagnosis on anomalous origin of left coronary artery from pulmonary artery(ALCAPA)by echocardiography.Methods This is a retrospective study.Patients with ALCAPA who underwent surgical treatment in Union Hospital,Tongji Medical College,Huazhong University of Science and Technology from August 2008 to December 2021 were included.

A multicenter cross-sectional study on the multidimensional clinical manifestations of irritable bowel syndrome

Objective To assess the differences in multidimensional clinical manifestations between patients with irritable bowel syndrome (IBS) matching the RomeⅢcriteria but not matching RomeⅣand IBS patients matching the RomeⅣcriteria,among patients diagnosed with IBS according to RomeⅢcriteria.Methods From November 2016 to October 2017.

Long-term follow-up of humanized and murine CD19 CAR-T-cell therapy for B-cell acute lymphoblastic leukemia

Objective To explore the differences in safety and short-and long-term follow-up efficacy between humanized and murine CD19 CAR-T-cells for treating relapsed and refractory B-ALL.Methods Clinical data of 80 patients with R/R B-ALL treated with CD19-targeted CAR-Tcells at the Union Hospital of Tongji Medical College of Huazhong University of Science and Technology between May 2016 and March 2023 were analyzed.

Key microbial monitoring and clinical analysis of bloodstream infections and CRO colonization after hematopoietic stem cell transplantation in hematological patients

Objective To investigate the distribution and clinical characteristics of pathogenic bacteria following hematopoietic stem cell transplantation (HSCT),as well as to provide a preliminary research foundation for key microbial monitoring,and clinical diagnosis and treatment of infections after HSCT in hematological patients.

Optimizing Bayesian penalized likelihood algorithm for low count PET reconstruction to simulate low dose PET imaging

Objective To study whether Bayesian penalized likelihood (BPL) and its optimized reconstruction algorithm can improve the reconstructed image quality of low count total-body PET.Methods Eight patients(5 males,3 females,age (67.2±6.3) years) who underwent hybrid^(18)F-FDG PET/MR total-body scans at Department of Nuclear Medicine in Union Hospital.

Levels of main platelet thrombin receptors in older chronic haemodialysis patients

To the Editor： We read with great interest the article by Yu et al, in which they summarized the recent progress and prospects of patients with kidney disease in China. This is a very interesting paper on kidney disease since the treatment of end-stage renal disease, especially the right time to haemodialysis （HD）, has been controversial over the past decades.

Association of liver enzymes with metabolic syndrome( MS) and pre-MS in adults aged over 40 years old

Objective To explore the relationship of metabolic syndrome(MS)and pre-MS with serum liver enzymes.Methods A total of 9 418 individuals aged over 40 years old were enrolled in the cross-sectional survey in Yiling District of Yichang City.The levels of alanine transaminase(ALT),aspartate aminotransferase(AST),γ-glu-

The clinical application of combined dualenergy CT pulmonary angiography and indirect venography

Objective To assess the usefulness of combined dual-energy CT pulmonary angiography (DE-CTPA) and indirect CT venography(CTV) in the diagnosis of venous thromboembolism(VTE). Methods Forty-nine patients with leg swelling suspected of pulmonary embolism(PE) underwent both DE-CTPA combined with CTV and

The effect of NALP3 inflammasome pathway in visceral adipose tissue on the development of insulin resistance in catch-up growth after food restriction in rats

Objective To investigate the effects of catch-up growth(CUG)on the natch domain,leucine-rich repeat and PVD-containing protein 3(NALP3)inflammasome pathway in visceral adipose tissue(VAT)and the mechanism of insulin resistance(IR)in CUG.Methods Sprague-Dawley rats were randomly divided into the normal chow(NC)and the catch-up growth group(CUG).General characteristics,glucose infusion rate60-120(GIR60-120)in hyperinsulinemic-euglycemic clamp and expression of NALP3 inflammasome,caspase1(p10)

Autoantibodies against α_1 adrenergic receptor related with cardiac remodeling in hypertensive patients by clinical observation

Objective To investigate the effects of autoantibodies against a adrenergic receptor on cardiac remodeling in patients with hypertension. Methods Five hundred and fifty three patients with hypertension in our hospital were selected. The autoantibodies againstα1 adrenergic receptor in sera of donor were detected by ELISA, and the Results of echocardiography were recorded.

A randomized controlled clinical study of lower-dose peginterferon alfa-2b in combination with ribavirin in the treatment of chronic hepatitis C

Objective To compare the efficacy and safety of lower-dose peginterferon alfa-2b plus ribavirin versus standard interferon alfa-2b plus ribavirin for treatment of chronic hepatitis C in China. Methods 192 patients with chronic hepatitis C were assigned peginterferon alfa-2b 0.5μg/kg each week plus ribavirin 750～1050 mg/d or standard interferon alfa-2b 3 MIU TIW plus ribavirin 750～1050 mg/d for 48-week treatment and 24-week

Effects of different cytokines on proliferation and apoptosis of pleural mesothelial cells in human Mycobacterium tuberculosis infection

Objective To investigate the effects of different cytokines (IL-22,IL-17,IFN-γ) on proliferation and apoptosis of human pleural mesothelial cells (PMC) during Mycobacterium tuberculosis infection.Methods

Efficacy and safety of domestic dasatinib as second-line treatment for chronic myeloid leukemia patients in the chronic phase

Objective To investigate the efficiency and safety of domestic tyrosine kinase inhibitor(TKI)dasatinib(Yinishu)as second-line treatment for patients with chronic myeloid leukemia in chronic phase(CML-CP).Methods A retrospective analysis of clinical data of CML-CP patients who received domestic dasatinib as second-line treatment in the CML collaborative group hospitals of Hubei province from March 2016 to July 2018 was performed.The optimal response rate,the cumulative complete cytogenetic response(CCyR),the cumulative major molecular responses(MMR),progression free survival(PFS),event free survival(EFS)and adverse effects(AEs)of the patients were assessed at 3,6 and 12 months of treatment.Results A total of 83 CML-CP patients were enrolled in this study.The median followup time was 23 months.The optimal response rates at 3,6 and 12 months in 83 CML-CP patients treated with dasatinib were 77.5%(54/71),72.6%(61/75)and 60.7%(51/69),respectively.By the end of followup,the cumulative CCyR and MMR rates were 65.5%(55/80)and 57.1%(48/73),respectively.The median time to achieve CCyR and MMR was 3 months.During follow-up time,the PFS rate was 94.0%(79/83)and the EFS rate was 77.4%(65/83).The most common non-hematological AEs of dasatinib were edema(32.5%),rash itching(18.1%)and fatigue(13.3%).The common hematological AEs of dasatinib were thrombocytopenia(31.3%),leukopenia(19.3%)and anemia(6.0%).Conclusion Domestic dasatinib was effective and safe as the second-line treatment of CML-CP patients and it can be used as an option for CML-CP patients.

Infectious complications following chimeric antigen receptor T-cell therapy for a hematologic malignancy within 28 days

Objective To explore the incidence,clinical and microbiological characteristics and risk factors of infection in patients with acute lymphoblastic(ALL),nonHodgkin lymphoma(NHL),and multiple myeloma(MM)within 28 days after CAR-T cell infusion,and to provide data support for early identification of infection and the rational use of antibacterial drugs in these patients.

Pretargeted immunoPET imaging with epidermal growth factor receptor monoclonal antibody in mouse models

Objective To explore the feasibility of pretargeting technique for immunoPET with epidermal growth factor receptor(EGFR)monoclonal antibody in EGFR positive/negative tumor bearing mice.Methods CetuximabTrans-cyclooctene(TCO)was obtained by modifying Cetuximab with TCON-hydroxysuccinimide(NHS).2,2′-((6-amino-1-(4,7-bis-(carboxymethyl)-1,4,7-triazonan-1-yl)hexan-2-yl)azanediyl)-diacetic acid(LNETA)was used as a chelating agent to prepare the radioligandGa-L-NETA-tetrazine(Tz).