To optimize the preparation process of chitosan microspheres and study its loading capacity, chitosan microsphere was prepared by crosslinking with glutaraldehyde, and bovine serum albumin （BSA） was absorbed onto ch...To optimize the preparation process of chitosan microspheres and study its loading capacity, chitosan microsphere was prepared by crosslinking with glutaraldehyde, and bovine serum albumin （BSA） was absorbed onto chitosan microsphere. Scanning electron microscope （SEM）, Fourier transform infrared spectroscopy （FITR）, TA instruments and zeta potentiometer analyzer were used to characterize the parameters with respect to size, thermal characters, morphology, and zeta potential of the microspheres. The loading capability and in vitro release tests were carried out. The results showed that chitosan microsphere with particle size less than 10 μm and positively charged （＋25.97±0.56 mV） can be obtained under the aldehyde group to amino group ratio at 1：1. A loading capacity of BSA at 28.63±0.15 g/100 g with corresponding loading efficiency at 72.01±1.44% was obtained for chitosan microsphere. In vitro test revealed a burst release followed by sustained-release profile.展开更多
基金Funded by the National Natural Science Foundation of China (Nos.31000773 and 31072119)Key Scientific and Technological Planning Project of Harbin (No.2009AA6CN125)Innovation Foundation of Harbin (No.2010RFQXN091)
文摘To optimize the preparation process of chitosan microspheres and study its loading capacity, chitosan microsphere was prepared by crosslinking with glutaraldehyde, and bovine serum albumin （BSA） was absorbed onto chitosan microsphere. Scanning electron microscope （SEM）, Fourier transform infrared spectroscopy （FITR）, TA instruments and zeta potentiometer analyzer were used to characterize the parameters with respect to size, thermal characters, morphology, and zeta potential of the microspheres. The loading capability and in vitro release tests were carried out. The results showed that chitosan microsphere with particle size less than 10 μm and positively charged （＋25.97±0.56 mV） can be obtained under the aldehyde group to amino group ratio at 1：1. A loading capacity of BSA at 28.63±0.15 g/100 g with corresponding loading efficiency at 72.01±1.44% was obtained for chitosan microsphere. In vitro test revealed a burst release followed by sustained-release profile.
