Primate sanctuaries across Africa play a pivotal role in the rescue and rehabilitation of confiscated and rescued wild primates, many of whom have had extensive contact with humans prior to their arrival and throughou...Primate sanctuaries across Africa play a pivotal role in the rescue and rehabilitation of confiscated and rescued wild primates, many of whom have had extensive contact with humans prior to their arrival and throughout the rehabilitation process, heightening the risk of disease transmission. While tuberculosis is not naturally occurring in free-living chimpanzees, it has been extensively observed in captive primates that have been in close proximity to humans or other captive primates infected with Mycobacterium tuberculosis. This case report delves into an outbreak of extra-pulmonary tuberculosis among juvenile chimpanzees within a sanctuary, detailing the associated diagnostic challenges and treatment approaches. The five cases had close contact with a caregiver infected with tuberculosis, subsequently transmitting the infection to other in-contact chimpanzees. Prolonged treatment, employing the human protocol of quadri-therapy (rifampicin, isoniazid, pyrazinamide, and ethambutol), followed by bi-therapy (rifampicin and isoniazid), resulted in complete resolution for all five cases. These cases underscore the critical importance of maintaining high levels of biosecurity, implementing effective quarantine measures, and adhering to strict hygiene practices when working with non-human primates.展开更多
Spices and aromatic plants are products of plant origin used in food. They are used for the preparation of remedies, for seasoning dishes or for preserving food. This review takes stock of the diversity of spices and ...Spices and aromatic plants are products of plant origin used in food. They are used for the preparation of remedies, for seasoning dishes or for preserving food. This review takes stock of the diversity of spices and aromatic herbs, the chemical composition, the different properties and forms of use of six spices and aromatic herbs commonly used in Benin and around the world. These are Zingiber officinalis (ginger), Curcuma longa (curcuma), Syzygium aromaticum (clove) and three aromatic herbs Petroselinum crispum (parsley), Rosmarinus officinalis (rosemary), and Laurus nobilis (laurel). The methodology used is that of documentary research oriented towards the consultation of previous scientific documents that have highlighted the different pharmacological activities of the different species of spices and aromatic plants targeted. It is important to note that more than twenty plant species are used as spices and aromatic plants in Benin and around the world. Chemically, these different spices and aromatic herbs contain certain secondary metabolites such as flavonoids, tannins, coumarins, alkaloids, steroids, terpenes, saponins, and polyphenols. This diversity of secondary metabolites alone or in a possible synergy may be responsible for many beneficial properties attributed to spices and aromatic herbs.展开更多
This study has been conducted to analyze the chemical composition and antimicrobial, antioxidant and antiplasmodial properties of ethanol extracts of Pycnostachys erici-rosenii R.E.Fr and Leucas martinicensis (Jack.) ...This study has been conducted to analyze the chemical composition and antimicrobial, antioxidant and antiplasmodial properties of ethanol extracts of Pycnostachys erici-rosenii R.E.Fr and Leucas martinicensis (Jack.) R.Br that are not yet well known. The HPLC-DAD-MS-ESI<sup>+</sup> method was used to investigate the chemical profile, the disc diffusion and microdilution method for the antimicrobial activities, the DPPH and FRAP assays for antioxidant property and SYBR Green I-based growth inhibition assay on Pf 3D7 (CQ-sensitive) strain for antiplasmodial properties. The results indicated significant amounts of hydroxybenzoic acid, flavonol and hydroxycinnamic acid in the two species. The total polyphenols analysis gave 209.0 ± 16.1 mg GAE/100g DW for P. erici-rosenii and 175.3 ± 1.6 mg GAE/100g DW for L. martinicensis while for total flavonoids analysis 33.4 ± 1.8 mg TE/100g DW was found for P. erici-rosenii and 39.6 ± 0.6 mg TE/100g DW for L. martinicensis. Moreover, antimicrobial tests revealed 0.125 mg/mL on E. faecalis ATCC25922 for ethanol extracts P. erici-rosenii and L. martinicensis. The antioxidant assays revealed IC<sub>50</sub> 56.17 μg/mL and 78.43 μg/mL for DPPH assay, and 112.03 μM TE/100mL and 90.46 μM TE/100mL) for FRAP assay for ethanol extracts of P. erici-rosenii and L. martinicensis respectively. Finally, for Antiplasmodial activity, IC<sub>50</sub> was 4.07 μg/mL for P. erici-rosenii and 12.29 μg/mL for L. martinicensis. Therefore, the antimicrobial, the antioxidant, and the antiplasmodial results suggest that the ethanolic extracts of the aerial parts of P. erici-rosenii and L. martinicensis contain compounds with antimicrobial, antioxidant, and antiplasmodial properties, which can be searched in new drug discovery for treatment of infectious diseases.展开更多
BACKGROUND Cachexia is responsible for the low quality of life in pancreatic adenocarcinoma(PDAC).The rapid disease progression and patient deterioration seems related to perineural invasion,but the relationship betwe...BACKGROUND Cachexia is responsible for the low quality of life in pancreatic adenocarcinoma(PDAC).The rapid disease progression and patient deterioration seems related to perineural invasion,but the relationship between cachexia and perineural invasion for the evolution of the disease has been rarely studied.As perineural invasion is difficult to be highlighted,a biomarker such as the neurotrophic factor Midkine(MK)which promotes the neuronal differentiation and the cell migration could be helpful.Also,Activin(ACV)has been described as cachexia related to PDAC.However,their role for assessing and predicting the disease course in daily practice is not known.AIM To assess the relationship between perineural invasion and cachexia and their biomarkers,MK and ACV,respectively,and their prognostic value.METHODS This study included prospectively enrolled patients with proven adenocarcinoma and a matched group of controls without any malignancies.Patients with other causes of malnutrition were excluded.The plasma levels of ACV and MK were analyzed using western blotting and were correlated with the clinicopathological features and survival data.These results were validated by immunohistochemical analyses of the pancreatic tumor tissue of the patients included in the study and a supplementary group of surgically resected specimens from patients with a benign disease.RESULTS The study comprised 114 patients with PDAC,125 controls and a supplementary group of 14 benign pancreatic tissue samples.ACV and MK were both overexpressed more frequently in the plasma of patients with PDAC than in the controls(63% vs 32% for ACV,P<0.001;47%vs 16%for MK,P<0.001),with similar levels in pancreatic tissue the MK protein expression was closely related to the advanced clinical stage(P=0.006),the presence of metastasis(P=0.04),perineural invasion(P=0.03)and diabetes(P=0.002),but with no influence on survival.No correlation between clinicopathological factors and ACV expression was noted.Cachexia,present in 19%of patients,was unrelated to ACV or MK level.Higher ACV expression was associated with a shorter survival(P=0.008).CONCLUSION The MK was a biomarker of perineural invasion,associated with tumor stage and diabetes,but without prognostic value as ACV.Cachexia was unrelated to perineural invasion,ACV level or survival.展开更多
Human action upon soil by tillage determines important morphological, physical-chemical and biological changes, with different intensities and evaluative directions. Nowadays, it is internationally accepted the fact t...Human action upon soil by tillage determines important morphological, physical-chemical and biological changes, with different intensities and evaluative directions. Nowadays, it is internationally accepted the fact that global climatic changes are the results of human intervention in the bio-geo-chemical water and material cycle, and the sequestration of carbon in soil is considered an important intervention to limit these changes. Carbon sequestration in soil is net advantageous, improving the productivity and sustainability. The more the organic content in soil is higher the better soil aggregation is. The soil without organic content is compact. This reduces its capacity to infiltrate water, nutrients solubility and productivity, and that way it reduces the soil capacity for carbon sequestration. Organic matter is an extremely important constituent of soils and is vital to many of the hydrological, biological and chemical reactions required for sustaining plant life. We present the influence of conventional plough tillage system on soil, water and organic matter conservation in comparison with an alternative minimum tillage system (paraplow, chisel plow and rotary harrow). The application of minimum tillage systems increased the organic matter content 0.8% to 22.1% and water stabile aggregate content from 1.3% to 13.6%, in the 0 - 30 cm depth, as compared to the classical system. For the organic matter content and the wet aggregate stability, the statistical analysis of the data showed, increasing positive significance of minimum systems. While the soil fertility and the wet aggregate stability were initially low, the effect of conservation practices on the soil features resulted in a positive impact on the water permeability of the soil. Availability of soil moisture during the crop growth resulted in better plant water status. Subsequent release of conserved soil water regulated proper plant water status, soil structure, and lowered soil pene-trometer resistance.展开更多
The objective of this research was to determine the capacity of a soil tillage system in soil conservation,in productivity and in energy efficiency.The minimum tillage and no-tillage systems represent good alternative...The objective of this research was to determine the capacity of a soil tillage system in soil conservation,in productivity and in energy efficiency.The minimum tillage and no-tillage systems represent good alternatives to the conventional(plough)system of soil tillage,due to their conservation effects on soil and to the good production of crops(Maize,96%-98%of conventional tillage for minimum tillage,and 99.8%of conventional tillage for no till;Soybeans,103%-112%of conventional tillage for minimum tillage and 117%of conventional tillage for no till;Wheat,93%-97%of conventional tillage for minimum tillage and 117%of conventional tillage for no till.The choice of the right soil tillage system for crops in rotation help reduce energy consumption,thus for maize:97%-98%energy consumption of conventional tillage when using minimum tillage and 91%when using no-tillage;for soybeans:98%energy consumption of conventional tillage when using minimum tillage and 93 when using no-tillage;for wheat:97%-98%energy consumption of conventional tillage when using minimum tillage and 92%when using no-tillage.Energy efficiency is in relation to reductions in energy use,but also might include the efficiency and impact of the tillage system on the cultivated plant.For all crops in rotation,energy efficiency(energy produced from 1 MJ consumed)was the best in no-tillage-10.44 MJ ha-1 for maize,6.49 MJ ha-1 for soybean,and 5.66 MJ ha-1 for wheat.An analysis of energy-efficiency in agricultural systems includes the energy consumed-energy produced-energy yield comparisons,but must be supplemented by soil energy efficiency,based on the conservative effect of the agricultural system.Only then will the agricultural system be sustainable,durable in agronomic,economic and ecological terms.The implementation of minimum and no-tillage soil systems has increased the organic matter content from 2%to 7.6%and water stable aggregate content from 5.6%to 9.6%,at 0-30 cm depth,as compared to the conventional system.Accumulated water supply was higher(with 12.4%-15%)for all minimum and no-tillage systems and increased bulk density values by 0.01%-0.03%(no significant difference)While the soil fertility and the wet aggregate stability have initially been low,the effect of conservation practices on the soil characteristics led to a positive impact on the water permeability in the soil.Availability of soil moisture during the crop growth period led to a better plant watering condition.Subsequent release of conserved soil water regulated the plant water condition and soil structure.展开更多
文摘Primate sanctuaries across Africa play a pivotal role in the rescue and rehabilitation of confiscated and rescued wild primates, many of whom have had extensive contact with humans prior to their arrival and throughout the rehabilitation process, heightening the risk of disease transmission. While tuberculosis is not naturally occurring in free-living chimpanzees, it has been extensively observed in captive primates that have been in close proximity to humans or other captive primates infected with Mycobacterium tuberculosis. This case report delves into an outbreak of extra-pulmonary tuberculosis among juvenile chimpanzees within a sanctuary, detailing the associated diagnostic challenges and treatment approaches. The five cases had close contact with a caregiver infected with tuberculosis, subsequently transmitting the infection to other in-contact chimpanzees. Prolonged treatment, employing the human protocol of quadri-therapy (rifampicin, isoniazid, pyrazinamide, and ethambutol), followed by bi-therapy (rifampicin and isoniazid), resulted in complete resolution for all five cases. These cases underscore the critical importance of maintaining high levels of biosecurity, implementing effective quarantine measures, and adhering to strict hygiene practices when working with non-human primates.
文摘Spices and aromatic plants are products of plant origin used in food. They are used for the preparation of remedies, for seasoning dishes or for preserving food. This review takes stock of the diversity of spices and aromatic herbs, the chemical composition, the different properties and forms of use of six spices and aromatic herbs commonly used in Benin and around the world. These are Zingiber officinalis (ginger), Curcuma longa (curcuma), Syzygium aromaticum (clove) and three aromatic herbs Petroselinum crispum (parsley), Rosmarinus officinalis (rosemary), and Laurus nobilis (laurel). The methodology used is that of documentary research oriented towards the consultation of previous scientific documents that have highlighted the different pharmacological activities of the different species of spices and aromatic plants targeted. It is important to note that more than twenty plant species are used as spices and aromatic plants in Benin and around the world. Chemically, these different spices and aromatic herbs contain certain secondary metabolites such as flavonoids, tannins, coumarins, alkaloids, steroids, terpenes, saponins, and polyphenols. This diversity of secondary metabolites alone or in a possible synergy may be responsible for many beneficial properties attributed to spices and aromatic herbs.
文摘This study has been conducted to analyze the chemical composition and antimicrobial, antioxidant and antiplasmodial properties of ethanol extracts of Pycnostachys erici-rosenii R.E.Fr and Leucas martinicensis (Jack.) R.Br that are not yet well known. The HPLC-DAD-MS-ESI<sup>+</sup> method was used to investigate the chemical profile, the disc diffusion and microdilution method for the antimicrobial activities, the DPPH and FRAP assays for antioxidant property and SYBR Green I-based growth inhibition assay on Pf 3D7 (CQ-sensitive) strain for antiplasmodial properties. The results indicated significant amounts of hydroxybenzoic acid, flavonol and hydroxycinnamic acid in the two species. The total polyphenols analysis gave 209.0 ± 16.1 mg GAE/100g DW for P. erici-rosenii and 175.3 ± 1.6 mg GAE/100g DW for L. martinicensis while for total flavonoids analysis 33.4 ± 1.8 mg TE/100g DW was found for P. erici-rosenii and 39.6 ± 0.6 mg TE/100g DW for L. martinicensis. Moreover, antimicrobial tests revealed 0.125 mg/mL on E. faecalis ATCC25922 for ethanol extracts P. erici-rosenii and L. martinicensis. The antioxidant assays revealed IC<sub>50</sub> 56.17 μg/mL and 78.43 μg/mL for DPPH assay, and 112.03 μM TE/100mL and 90.46 μM TE/100mL) for FRAP assay for ethanol extracts of P. erici-rosenii and L. martinicensis respectively. Finally, for Antiplasmodial activity, IC<sub>50</sub> was 4.07 μg/mL for P. erici-rosenii and 12.29 μg/mL for L. martinicensis. Therefore, the antimicrobial, the antioxidant, and the antiplasmodial results suggest that the ethanolic extracts of the aerial parts of P. erici-rosenii and L. martinicensis contain compounds with antimicrobial, antioxidant, and antiplasmodial properties, which can be searched in new drug discovery for treatment of infectious diseases.
基金partially funded by the“Iuliu Hatieganu”University of Medicine and Pharmacy,Cluj-Napoca,through the Doctoral Research Project-2015(No.7690/36/15.04.2016)
文摘BACKGROUND Cachexia is responsible for the low quality of life in pancreatic adenocarcinoma(PDAC).The rapid disease progression and patient deterioration seems related to perineural invasion,but the relationship between cachexia and perineural invasion for the evolution of the disease has been rarely studied.As perineural invasion is difficult to be highlighted,a biomarker such as the neurotrophic factor Midkine(MK)which promotes the neuronal differentiation and the cell migration could be helpful.Also,Activin(ACV)has been described as cachexia related to PDAC.However,their role for assessing and predicting the disease course in daily practice is not known.AIM To assess the relationship between perineural invasion and cachexia and their biomarkers,MK and ACV,respectively,and their prognostic value.METHODS This study included prospectively enrolled patients with proven adenocarcinoma and a matched group of controls without any malignancies.Patients with other causes of malnutrition were excluded.The plasma levels of ACV and MK were analyzed using western blotting and were correlated with the clinicopathological features and survival data.These results were validated by immunohistochemical analyses of the pancreatic tumor tissue of the patients included in the study and a supplementary group of surgically resected specimens from patients with a benign disease.RESULTS The study comprised 114 patients with PDAC,125 controls and a supplementary group of 14 benign pancreatic tissue samples.ACV and MK were both overexpressed more frequently in the plasma of patients with PDAC than in the controls(63% vs 32% for ACV,P<0.001;47%vs 16%for MK,P<0.001),with similar levels in pancreatic tissue the MK protein expression was closely related to the advanced clinical stage(P=0.006),the presence of metastasis(P=0.04),perineural invasion(P=0.03)and diabetes(P=0.002),but with no influence on survival.No correlation between clinicopathological factors and ACV expression was noted.Cachexia,present in 19%of patients,was unrelated to ACV or MK level.Higher ACV expression was associated with a shorter survival(P=0.008).CONCLUSION The MK was a biomarker of perineural invasion,associated with tumor stage and diabetes,but without prognostic value as ACV.Cachexia was unrelated to perineural invasion,ACV level or survival.
文摘Human action upon soil by tillage determines important morphological, physical-chemical and biological changes, with different intensities and evaluative directions. Nowadays, it is internationally accepted the fact that global climatic changes are the results of human intervention in the bio-geo-chemical water and material cycle, and the sequestration of carbon in soil is considered an important intervention to limit these changes. Carbon sequestration in soil is net advantageous, improving the productivity and sustainability. The more the organic content in soil is higher the better soil aggregation is. The soil without organic content is compact. This reduces its capacity to infiltrate water, nutrients solubility and productivity, and that way it reduces the soil capacity for carbon sequestration. Organic matter is an extremely important constituent of soils and is vital to many of the hydrological, biological and chemical reactions required for sustaining plant life. We present the influence of conventional plough tillage system on soil, water and organic matter conservation in comparison with an alternative minimum tillage system (paraplow, chisel plow and rotary harrow). The application of minimum tillage systems increased the organic matter content 0.8% to 22.1% and water stabile aggregate content from 1.3% to 13.6%, in the 0 - 30 cm depth, as compared to the classical system. For the organic matter content and the wet aggregate stability, the statistical analysis of the data showed, increasing positive significance of minimum systems. While the soil fertility and the wet aggregate stability were initially low, the effect of conservation practices on the soil features resulted in a positive impact on the water permeability of the soil. Availability of soil moisture during the crop growth resulted in better plant water status. Subsequent release of conserved soil water regulated proper plant water status, soil structure, and lowered soil pene-trometer resistance.
基金performed under the frame of the Partnership in priority domains-PNII,developed with the support of MEN-UEFISCDI,project no.PN-II-PT-PCCA-2013-4-0015:Expert System for Risk Monitoring in Agriculture and Adaptation of Conservative Agricultural Technologies to Climate Change.
文摘The objective of this research was to determine the capacity of a soil tillage system in soil conservation,in productivity and in energy efficiency.The minimum tillage and no-tillage systems represent good alternatives to the conventional(plough)system of soil tillage,due to their conservation effects on soil and to the good production of crops(Maize,96%-98%of conventional tillage for minimum tillage,and 99.8%of conventional tillage for no till;Soybeans,103%-112%of conventional tillage for minimum tillage and 117%of conventional tillage for no till;Wheat,93%-97%of conventional tillage for minimum tillage and 117%of conventional tillage for no till.The choice of the right soil tillage system for crops in rotation help reduce energy consumption,thus for maize:97%-98%energy consumption of conventional tillage when using minimum tillage and 91%when using no-tillage;for soybeans:98%energy consumption of conventional tillage when using minimum tillage and 93 when using no-tillage;for wheat:97%-98%energy consumption of conventional tillage when using minimum tillage and 92%when using no-tillage.Energy efficiency is in relation to reductions in energy use,but also might include the efficiency and impact of the tillage system on the cultivated plant.For all crops in rotation,energy efficiency(energy produced from 1 MJ consumed)was the best in no-tillage-10.44 MJ ha-1 for maize,6.49 MJ ha-1 for soybean,and 5.66 MJ ha-1 for wheat.An analysis of energy-efficiency in agricultural systems includes the energy consumed-energy produced-energy yield comparisons,but must be supplemented by soil energy efficiency,based on the conservative effect of the agricultural system.Only then will the agricultural system be sustainable,durable in agronomic,economic and ecological terms.The implementation of minimum and no-tillage soil systems has increased the organic matter content from 2%to 7.6%and water stable aggregate content from 5.6%to 9.6%,at 0-30 cm depth,as compared to the conventional system.Accumulated water supply was higher(with 12.4%-15%)for all minimum and no-tillage systems and increased bulk density values by 0.01%-0.03%(no significant difference)While the soil fertility and the wet aggregate stability have initially been low,the effect of conservation practices on the soil characteristics led to a positive impact on the water permeability in the soil.Availability of soil moisture during the crop growth period led to a better plant watering condition.Subsequent release of conserved soil water regulated the plant water condition and soil structure.
