Xerostomia is a severe side effect of radiation therapy in head and neck cancer patients. To date, no satisfactory treatment option has been established. Because mesenchymal stem cells（MSCs） have been identified as ...Xerostomia is a severe side effect of radiation therapy in head and neck cancer patients. To date, no satisfactory treatment option has been established. Because mesenchymal stem cells（MSCs） have been identified as a potential treatment modality, we aimed to evaluate stem cell distribution following intravenous and intraglandular injections using a surgical model of salivary gland damage and to analyse the effects of MSC injections on the recruitment of immune cells. The submandibular gland ducts of rats were surgically ligated. Syngeneic adult MSCs were isolated, immortalised by simian virus 40（SV40） large T antigen and characterized by flow cytometry. MSCs were injected intravenously and intraglandularly. After 1, 3 and 7 days, the organs of interest were analysed for stem cell recruitment. Inflammation was analysed by immunohistochemical staining. We were able to demonstrate that, after intravenous injection, MSCs were recruited to normal and damaged submandibular glands on days 1, 3 and 7. Unexpectedly, stem cells were recruited to ligated and non-ligated glands in a comparable manner. After intraglandular injection of MSCs into ligated glands, the presence of MSCs, leucocytes and macrophages was enhanced, compared to intravenous injection of stem cells. Our data suggest that injected MSCs were retained within the inflamed glands, could become activated and subsequently recruited leucocytes to the sites of tissue damage.展开更多
Bivariate map visualizations use different encodings to visualize two variables but comparison across multiple encodings is challenging.Compared to a univariate visualization,it is significantly harder to read regiona...Bivariate map visualizations use different encodings to visualize two variables but comparison across multiple encodings is challenging.Compared to a univariate visualization,it is significantly harder to read regional differences and spot geographical outliers.Especially targeting inexperienced users of visualizations,we advocate the use of natural language text for augmenting map visualizations and understanding the relationship between two geo-statistical variables.We propose an approach that selects interesting findings from data analysis,generates a respective text and visualization,and integrates both into a single document.The generated reports interactively link the visualization with the textual narrative.Users can get additional explanations and have the ability to compare different regions.The text generation process is flexible and adapts to various geographical and contextual settings based on small sets of parameters.We showcase this flexibility through a number of application examples.展开更多
基金supported by the Novartis Foundation for Therapeutic Research
文摘Xerostomia is a severe side effect of radiation therapy in head and neck cancer patients. To date, no satisfactory treatment option has been established. Because mesenchymal stem cells（MSCs） have been identified as a potential treatment modality, we aimed to evaluate stem cell distribution following intravenous and intraglandular injections using a surgical model of salivary gland damage and to analyse the effects of MSC injections on the recruitment of immune cells. The submandibular gland ducts of rats were surgically ligated. Syngeneic adult MSCs were isolated, immortalised by simian virus 40（SV40） large T antigen and characterized by flow cytometry. MSCs were injected intravenously and intraglandularly. After 1, 3 and 7 days, the organs of interest were analysed for stem cell recruitment. Inflammation was analysed by immunohistochemical staining. We were able to demonstrate that, after intravenous injection, MSCs were recruited to normal and damaged submandibular glands on days 1, 3 and 7. Unexpectedly, stem cells were recruited to ligated and non-ligated glands in a comparable manner. After intraglandular injection of MSCs into ligated glands, the presence of MSCs, leucocytes and macrophages was enhanced, compared to intravenous injection of stem cells. Our data suggest that injected MSCs were retained within the inflamed glands, could become activated and subsequently recruited leucocytes to the sites of tissue damage.
文摘Bivariate map visualizations use different encodings to visualize two variables but comparison across multiple encodings is challenging.Compared to a univariate visualization,it is significantly harder to read regional differences and spot geographical outliers.Especially targeting inexperienced users of visualizations,we advocate the use of natural language text for augmenting map visualizations and understanding the relationship between two geo-statistical variables.We propose an approach that selects interesting findings from data analysis,generates a respective text and visualization,and integrates both into a single document.The generated reports interactively link the visualization with the textual narrative.Users can get additional explanations and have the ability to compare different regions.The text generation process is flexible and adapts to various geographical and contextual settings based on small sets of parameters.We showcase this flexibility through a number of application examples.
