Antibacterial extract-coated catheters are promising alternatives to their conventional counterparts,but their hemocompatibility and thermal stability must be studied.Nosocomial bacteria have developed resistance to c...Antibacterial extract-coated catheters are promising alternatives to their conventional counterparts,but their hemocompatibility and thermal stability must be studied.Nosocomial bacteria have developed resistance to conventional antibiotics.Herein,the minimum inhibitory but non-hemolytic concentration(MIC-NH)and the thermal stability of Larrea tridentata(L.tridentata)and Origanum vulgare(O.vulgare)extract-coated catheters were studied.Besides,plasma pretreatment was performed to enhance the extract adhesion.Briefly,the extract-coated catheters prevent Staphylococcus aureus colonization without causing hemolysis by using L.tridentata and O.vulgare extracts at MIC-NH(5000 and 2500μg ml~(-1),respectively).Moreover,it has been discovered that the extract coating and plasma treatment improved the thermal stability and the extract adhesion,respectively.Thus,this study provides evidence of alternative antibacterial but non-hemolytic extract-coated catheters.展开更多
Introduction: Thymus vulgaris is used in traditional medicine to treat gastrointestinal diseases because of its antifungal, antibacterial, and antispasmodic activity. Objective: To verify whether Thymus vulgaris also ...Introduction: Thymus vulgaris is used in traditional medicine to treat gastrointestinal diseases because of its antifungal, antibacterial, and antispasmodic activity. Objective: To verify whether Thymus vulgaris also has antiprotozoal activity against Trichomonas vaginalis, Giardia lamblia and Entamoeba histolytica trophozoites. Materials and methods: Conventional cultures of parasites were measured on the third day during the logarithmic growth phase. The antiprotozoal activity of the methanol extract and its fractions were evaluated comparing growth in cultures with and without extracts. Next, the extract was fractionated by polarity-based partitioning. Then, the purity of each fraction was determined by thin layer chromatography (TLC). The percentage of growth inhibition was calculated with respect to untreated controls. The 50% inhibitory concentration (IC50) of each extract was calculated by PROBIT analysis. Results: We found that a methanol extract of the aerial parts of Thymus vulgaris, at 300 μg/mL, inhibited the in vitro growth of G. lamblia and T. vaginalis, while E. histolytica growth was poorly inhibited. The methanol extract was further separated into mixtures of ursolic, oleanolic, and betulinic acids. The IC50 values of ursolic acid against G. lamblia and T. vaginalis were 8.12 μg/mL and 5.51 μg/mL, respectively. Conclusions: The methanol extract fraction containing ursolic acid obtained from Thymus vulgaris has antiprotozoal activity against G. lamblia and T. vaginalis trophozoites.展开更多
基金the grant(No.CVU 859503)given to the first author to pursue his doctorate in Materials Science and Technology at the Autonomous University of Coahuila(UAdeC)FONCYT-Fund Destined to Promote the Development of Science and Technology in the State of Coahuila(No.COAH-2020-C14-C058)。
文摘Antibacterial extract-coated catheters are promising alternatives to their conventional counterparts,but their hemocompatibility and thermal stability must be studied.Nosocomial bacteria have developed resistance to conventional antibiotics.Herein,the minimum inhibitory but non-hemolytic concentration(MIC-NH)and the thermal stability of Larrea tridentata(L.tridentata)and Origanum vulgare(O.vulgare)extract-coated catheters were studied.Besides,plasma pretreatment was performed to enhance the extract adhesion.Briefly,the extract-coated catheters prevent Staphylococcus aureus colonization without causing hemolysis by using L.tridentata and O.vulgare extracts at MIC-NH(5000 and 2500μg ml~(-1),respectively).Moreover,it has been discovered that the extract coating and plasma treatment improved the thermal stability and the extract adhesion,respectively.Thus,this study provides evidence of alternative antibacterial but non-hemolytic extract-coated catheters.
文摘Introduction: Thymus vulgaris is used in traditional medicine to treat gastrointestinal diseases because of its antifungal, antibacterial, and antispasmodic activity. Objective: To verify whether Thymus vulgaris also has antiprotozoal activity against Trichomonas vaginalis, Giardia lamblia and Entamoeba histolytica trophozoites. Materials and methods: Conventional cultures of parasites were measured on the third day during the logarithmic growth phase. The antiprotozoal activity of the methanol extract and its fractions were evaluated comparing growth in cultures with and without extracts. Next, the extract was fractionated by polarity-based partitioning. Then, the purity of each fraction was determined by thin layer chromatography (TLC). The percentage of growth inhibition was calculated with respect to untreated controls. The 50% inhibitory concentration (IC50) of each extract was calculated by PROBIT analysis. Results: We found that a methanol extract of the aerial parts of Thymus vulgaris, at 300 μg/mL, inhibited the in vitro growth of G. lamblia and T. vaginalis, while E. histolytica growth was poorly inhibited. The methanol extract was further separated into mixtures of ursolic, oleanolic, and betulinic acids. The IC50 values of ursolic acid against G. lamblia and T. vaginalis were 8.12 μg/mL and 5.51 μg/mL, respectively. Conclusions: The methanol extract fraction containing ursolic acid obtained from Thymus vulgaris has antiprotozoal activity against G. lamblia and T. vaginalis trophozoites.
