目的探索血液筛查结果为HBsAg+&HBV DNA NR的HBV感染的血清学和分子生物学特性。方法通过重复核酸检测、PEG沉降病毒富集联合in-house的巢式PCR和实时荧光定量PCR,对HBsAg+&HBV DNA NR标本进行HBV DNA的确认、抗-HBc和HBsAg定...目的探索血液筛查结果为HBsAg+&HBV DNA NR的HBV感染的血清学和分子生物学特性。方法通过重复核酸检测、PEG沉降病毒富集联合in-house的巢式PCR和实时荧光定量PCR,对HBsAg+&HBV DNA NR标本进行HBV DNA的确认、抗-HBc和HBsAg定量检测,并将HBV序列与对照组HBV慢性感染和隐匿性感染序列进行比对分析。结果2011年1月~2020年12月,共检测标本792195份,筛选出HBsAg+&HBV DNA-标本53份(1∶14947)。获得S序列3份、Pre Core/Core序列4份,确认含有HBV DNA的标本有5份。Core区域发现独特氨基酸替换(P130T、P135Q/S、R151Q、G153S、S155F),可能对病毒包装、复制产生影响。结论血液筛查结果为HBsAg+&HBV DNA NR的血液存在极低水平的HBV DNA;低水平HBV DNA可能与Pre Core/Core区域的某些突变影响病毒复制有关。选择灵敏度更好的HBsAg和HBV DNA检测试剂能够进一步降低HBV经血传播的潜在风险。展开更多
BACKGROUND Oncocytic carcinoma of the thyroid is a rare disease,characterized by a poor prognosis and low response rate to radioiodine therapy.Crizotinib is a specific anaplastic lymphoma kinase(ALK)inhibitor,which wa...BACKGROUND Oncocytic carcinoma of the thyroid is a rare disease,characterized by a poor prognosis and low response rate to radioiodine therapy.Crizotinib is a specific anaplastic lymphoma kinase(ALK)inhibitor,which was initially developed in non-small cell lung cancer.Other solid tumors harboring a translocation in ALK have been described,such as renal carcinoma,thyroid,colorectal,ovarian cancers,and spitzoid melanoma.The research of ALK rearrangements in thyroid tumor is a promising therapeutic track,and treatments need to be explored.CASE SUMMARY We report the case of a 76-year-old woman with a history of multinodular goiter,who was hospitalized for impairment of her general condition.She was diagnosed with metastatic oncocytic thyroid cancer.Synchrone metastases were found:Multiple mediastinal lymphadenopathies,lytic bone lesions and bilateral mammary lumps.Fluorescence in situ hybridization analysis revealed an ALK rearrangement in 61%of cells.No other mutation was found.A tumor board discussion based on molecular characteristics of the tumor suggested initiating a daily treatment by crizotinib,a specific ALK inhibitor.A positron emission tomography scan performed 4 mo after the initiation of crizotinib showed a complete metabolic response.CONCLUSION This case highlights an unexpected efficacy of crizotinib in an ALK-rearranged thyroid tumor,and the need of further assessments.展开更多
In radar SAR interferometry (InSAR/DinSAR), a Tandem pair (ERS-1/2) is used for the realization of a DEM or the detection of deformations of a major event (e.g. earthquake) occurring between two acquisitions. The adva...In radar SAR interferometry (InSAR/DinSAR), a Tandem pair (ERS-1/2) is used for the realization of a DEM or the detection of deformations of a major event (e.g. earthquake) occurring between two acquisitions. The advantage of the Tandem pair is the time interval of 24 hours between the two captures. Generally, there is no temporal decorrelation considering the small difference in time between the acquisitions, which results in a good coherence. The coherence image is used indirectly in radar SAR interferometry as an indicator of the levels of confidence of the interferograms. It can be used in a direct way to identify and delimit the various movement zones which disturb the phase of the radar wave. The objective of this work is to show the utility and the effectiveness of the coherence image in the cartography of lakes and vegetation in a region at the Curvature of the Romanian Carpathians1. Between the two acquisitions dates of May 28 and 29, 1995, there was no geological process involving displacements in direction of the radar. This pair represents the only one among the other pairs which gave a good coherence for the whole scene. In the flat part, one notes a good coherence except for the lakes. They are well represented by very dark colours with zero coherence. In the part of high altitude, especially where there is vegetation, a bad coherence can be clearly seen. Using this image (coherence), one could distinguish the parts with vegetation in great movements due to the effects of the wind, therefore characterised by great heights. The coherence image thus makes it possible to delimit two natural elements in this area, the lakes and the vegetation. The lakes are well delimited, in particular when they are full as in the rain seasons. To show this utility and this effectiveness of the coherence image, we measured the surfaces and dimensions of the lakes based on the Tandem pair and to make a comparison with the existing data and to interpret the results obtained. The three lakes, located in the plain to the East and North-East of the Buza;u town, clearly appear in the coherence image.展开更多
In this article, the authors address the problem of optimal guidance of road network users. In the literature, several routing algorithms have been proposed under different approaches to solve this problem. Most adapt...In this article, the authors address the problem of optimal guidance of road network users. In the literature, several routing algorithms have been proposed under different approaches to solve this problem. Most adaptive algorithms for optimal path are based on the least expected travel time. Another approach has emerged named the SOTA （stochastic on time arrival）. This approach based on the idea of Frank （1969）, which aims to maximize the probability of arriving to a destination node parting from a given node in the network and with a given time budget. The authors＇ contribution consists here in extending this approach in order to introduce robustness towards path failure, in the guidance optimization. The authors propose a model that includes the existence as well as the performance of detours for selected paths, in the calculus of the travel time reliability. This new way of calculating travel time reliability guarantees a kind of robustness of the optimal guidance strategy.展开更多
文摘目的探索血液筛查结果为HBsAg+&HBV DNA NR的HBV感染的血清学和分子生物学特性。方法通过重复核酸检测、PEG沉降病毒富集联合in-house的巢式PCR和实时荧光定量PCR,对HBsAg+&HBV DNA NR标本进行HBV DNA的确认、抗-HBc和HBsAg定量检测,并将HBV序列与对照组HBV慢性感染和隐匿性感染序列进行比对分析。结果2011年1月~2020年12月,共检测标本792195份,筛选出HBsAg+&HBV DNA-标本53份(1∶14947)。获得S序列3份、Pre Core/Core序列4份,确认含有HBV DNA的标本有5份。Core区域发现独特氨基酸替换(P130T、P135Q/S、R151Q、G153S、S155F),可能对病毒包装、复制产生影响。结论血液筛查结果为HBsAg+&HBV DNA NR的血液存在极低水平的HBV DNA;低水平HBV DNA可能与Pre Core/Core区域的某些突变影响病毒复制有关。选择灵敏度更好的HBsAg和HBV DNA检测试剂能够进一步降低HBV经血传播的潜在风险。
文摘BACKGROUND Oncocytic carcinoma of the thyroid is a rare disease,characterized by a poor prognosis and low response rate to radioiodine therapy.Crizotinib is a specific anaplastic lymphoma kinase(ALK)inhibitor,which was initially developed in non-small cell lung cancer.Other solid tumors harboring a translocation in ALK have been described,such as renal carcinoma,thyroid,colorectal,ovarian cancers,and spitzoid melanoma.The research of ALK rearrangements in thyroid tumor is a promising therapeutic track,and treatments need to be explored.CASE SUMMARY We report the case of a 76-year-old woman with a history of multinodular goiter,who was hospitalized for impairment of her general condition.She was diagnosed with metastatic oncocytic thyroid cancer.Synchrone metastases were found:Multiple mediastinal lymphadenopathies,lytic bone lesions and bilateral mammary lumps.Fluorescence in situ hybridization analysis revealed an ALK rearrangement in 61%of cells.No other mutation was found.A tumor board discussion based on molecular characteristics of the tumor suggested initiating a daily treatment by crizotinib,a specific ALK inhibitor.A positron emission tomography scan performed 4 mo after the initiation of crizotinib showed a complete metabolic response.CONCLUSION This case highlights an unexpected efficacy of crizotinib in an ALK-rearranged thyroid tumor,and the need of further assessments.
文摘In radar SAR interferometry (InSAR/DinSAR), a Tandem pair (ERS-1/2) is used for the realization of a DEM or the detection of deformations of a major event (e.g. earthquake) occurring between two acquisitions. The advantage of the Tandem pair is the time interval of 24 hours between the two captures. Generally, there is no temporal decorrelation considering the small difference in time between the acquisitions, which results in a good coherence. The coherence image is used indirectly in radar SAR interferometry as an indicator of the levels of confidence of the interferograms. It can be used in a direct way to identify and delimit the various movement zones which disturb the phase of the radar wave. The objective of this work is to show the utility and the effectiveness of the coherence image in the cartography of lakes and vegetation in a region at the Curvature of the Romanian Carpathians1. Between the two acquisitions dates of May 28 and 29, 1995, there was no geological process involving displacements in direction of the radar. This pair represents the only one among the other pairs which gave a good coherence for the whole scene. In the flat part, one notes a good coherence except for the lakes. They are well represented by very dark colours with zero coherence. In the part of high altitude, especially where there is vegetation, a bad coherence can be clearly seen. Using this image (coherence), one could distinguish the parts with vegetation in great movements due to the effects of the wind, therefore characterised by great heights. The coherence image thus makes it possible to delimit two natural elements in this area, the lakes and the vegetation. The lakes are well delimited, in particular when they are full as in the rain seasons. To show this utility and this effectiveness of the coherence image, we measured the surfaces and dimensions of the lakes based on the Tandem pair and to make a comparison with the existing data and to interpret the results obtained. The three lakes, located in the plain to the East and North-East of the Buza;u town, clearly appear in the coherence image.
文摘In this article, the authors address the problem of optimal guidance of road network users. In the literature, several routing algorithms have been proposed under different approaches to solve this problem. Most adaptive algorithms for optimal path are based on the least expected travel time. Another approach has emerged named the SOTA （stochastic on time arrival）. This approach based on the idea of Frank （1969）, which aims to maximize the probability of arriving to a destination node parting from a given node in the network and with a given time budget. The authors＇ contribution consists here in extending this approach in order to introduce robustness towards path failure, in the guidance optimization. The authors propose a model that includes the existence as well as the performance of detours for selected paths, in the calculus of the travel time reliability. This new way of calculating travel time reliability guarantees a kind of robustness of the optimal guidance strategy.
