Neuromuscular junction is the main target for snakebites, which venoms act depending on the snake genus for paralyzing the prey （neurotoxicity） or for facilitating the digestion processes of the victim （myotoxicity...Neuromuscular junction is the main target for snakebites, which venoms act depending on the snake genus for paralyzing the prey （neurotoxicity） or for facilitating the digestion processes of the victim （myotoxicity）, and also as a defense mechanism against predators. In the present study, a mouse neuromuscular apparatus was used for testing the hexane fraction of Casearia gossypiosperma plant, that showed the better antiophidian ability than dichloromethane, ethyl acetate and methanol fractions, against the irreversible paralysis induced by two, Bothrops jararacussu （Bjssu, 40 μg/mL） and Crotalus durissus terrificus （Cdt, 10 μg/mL） snake venoms. All fractions were obtained by liquid-liquid partition from the C. gossypiosperma hydroalcoholic lyophilized extract. The preliminary chromatographic profile of this plant showed phenols and flavonols as active constituents, whereas hexane fraction expressed mainly 13-sitosterol and quercetin. In spite of hexane fraction protection （≌95% and 48% against Bjssu and Cdt, respectively）, isolately, only quercetin protected against the blockade-induced by Bjssu venom （65.5%）. This study showed that hexane fraction acts against these snake venoms by a synergistic phytocomplex mechanism.展开更多
This article was carried out to gather a collection of data extracted from several studies about the snake Lachesis muta muta(surucucu),encompassing in this information from the effects and properties of its venom,cas...This article was carried out to gather a collection of data extracted from several studies about the snake Lachesis muta muta(surucucu),encompassing in this information from the effects and properties of its venom,case report,treatment and even diagnosis.L.m.muta was addressed throughout the study by referring mainly to their venom,which in isolated fractions,as LHFI,LHFII,LmTX-I,among others,present several activities,among which stand out are:coagulant,hemorrhagic,proteolytic and neurotoxic.Besides,other studies involving its diagnosis,treatment,cytotoxicity,edema,inflammation,genotoxicity and mutagenicity have also been carried out.The bibliographic survey initially made,followed by the selection of collected data,was properly organized in the text using the narrative method,so that the findings could be chronologically distributed in a timeline.Therefore,it was possible to conclude that all these studies were and are of paramount importance to help understand the pathophysiology of envenoming by this snake,in addition to pointing out the Bothrops-Lachesis antivenom,opening a wide therapeutic window for numerous applications.展开更多
文摘Neuromuscular junction is the main target for snakebites, which venoms act depending on the snake genus for paralyzing the prey （neurotoxicity） or for facilitating the digestion processes of the victim （myotoxicity）, and also as a defense mechanism against predators. In the present study, a mouse neuromuscular apparatus was used for testing the hexane fraction of Casearia gossypiosperma plant, that showed the better antiophidian ability than dichloromethane, ethyl acetate and methanol fractions, against the irreversible paralysis induced by two, Bothrops jararacussu （Bjssu, 40 μg/mL） and Crotalus durissus terrificus （Cdt, 10 μg/mL） snake venoms. All fractions were obtained by liquid-liquid partition from the C. gossypiosperma hydroalcoholic lyophilized extract. The preliminary chromatographic profile of this plant showed phenols and flavonols as active constituents, whereas hexane fraction expressed mainly 13-sitosterol and quercetin. In spite of hexane fraction protection （≌95% and 48% against Bjssu and Cdt, respectively）, isolately, only quercetin protected against the blockade-induced by Bjssu venom （65.5%）. This study showed that hexane fraction acts against these snake venoms by a synergistic phytocomplex mechanism.
基金thank Fundação de AmparoàPesquisa do Estado de São Paulo(FAPESP,Grant No.2019/21147-6)for fellowship.
文摘This article was carried out to gather a collection of data extracted from several studies about the snake Lachesis muta muta(surucucu),encompassing in this information from the effects and properties of its venom,case report,treatment and even diagnosis.L.m.muta was addressed throughout the study by referring mainly to their venom,which in isolated fractions,as LHFI,LHFII,LmTX-I,among others,present several activities,among which stand out are:coagulant,hemorrhagic,proteolytic and neurotoxic.Besides,other studies involving its diagnosis,treatment,cytotoxicity,edema,inflammation,genotoxicity and mutagenicity have also been carried out.The bibliographic survey initially made,followed by the selection of collected data,was properly organized in the text using the narrative method,so that the findings could be chronologically distributed in a timeline.Therefore,it was possible to conclude that all these studies were and are of paramount importance to help understand the pathophysiology of envenoming by this snake,in addition to pointing out the Bothrops-Lachesis antivenom,opening a wide therapeutic window for numerous applications.
