Deepfake-generated fake faces,commonly utilized in identity-related activities such as political propaganda,celebrity impersonations,evidence forgery,and familiar fraud,pose new societal threats.Although current deepf...Deepfake-generated fake faces,commonly utilized in identity-related activities such as political propaganda,celebrity impersonations,evidence forgery,and familiar fraud,pose new societal threats.Although current deepfake generators strive for high realism in visual effects,they do not replicate biometric signals indicative of cardiac activity.Addressing this gap,many researchers have developed detection methods focusing on biometric characteristics.These methods utilize classification networks to analyze both temporal and spectral domain features of the remote photoplethysmography(rPPG)signal,resulting in high detection accuracy.However,in the spectral analysis,existing approaches often only consider the power spectral density and neglect the amplitude spectrum—both crucial for assessing cardiac activity.We introduce a novel method that extracts rPPG signals from multiple regions of interest through remote photoplethysmography and processes them using Fast Fourier Transform(FFT).The resultant time-frequency domain signal samples are organized into matrices to create Matrix Visualization Heatmaps(MVHM),which are then utilized to train an image classification network.Additionally,we explored various combinations of time-frequency domain representations of rPPG signals and the impact of attention mechanisms.Our experimental results show that our algorithm achieves a remarkable detection accuracy of 99.22%in identifying fake videos,significantly outperforming mainstream algorithms and demonstrating the effectiveness of Fourier Transform and attention mechanisms in detecting fake faces.展开更多
This study aims to improve control schemes for COVID-19 by a numerical model with estimation of parameters.We established a multi-level and multi-objective nonlinear SEIDR model to simulate the virus transmission.The ...This study aims to improve control schemes for COVID-19 by a numerical model with estimation of parameters.We established a multi-level and multi-objective nonlinear SEIDR model to simulate the virus transmission.The early spread in Japan was adopted as a case study.The first 96 days since the infection were divided into five stages with parameters estimated.Then,we analyzed the trend of the parameter value,age structure ratio,and the defined PCR test index(standardization of the scale of PCR tests).It was discovered that the self-healing rate and confirmed rate were linear with the age structure ratio and the PCR test index using the stepwise regression method.The transmission rates were related to the age structure ratio,PCR test index,and isolation efficiency.Both isolation measures and PCR test medical screening can effectively reduce the number of infected cases based on the simulation results.However,the strategy of increasing PCR test medical screening would encountered a bottleneck effect on the virus control when the index reached 0.3.The effectiveness of the policy would decrease and the basic reproduction number reached the extreme value at 0.6.This study gave a feasible combination for isolation and PCR test by simulation.The isolation intensity could be adjusted to compensate the insufficiency of PCR test to control the pandemic.展开更多
OBJECTIVE: To examine the influence that type of medical training and number of biopsies have on sensitivity of colposcopically guided biopsies. METHODS: Among 408 women with an adequate enrollment colposcopy and a di...OBJECTIVE: To examine the influence that type of medical training and number of biopsies have on sensitivity of colposcopically guided biopsies. METHODS: Among 408 women with an adequate enrollment colposcopy and a diagnosis of cervical intraepithelial neoplasia (CIN) 3 or cancer (CIN 3+ ) over 2 years in the Atypical Squamous Cells of Undetermined Significance/Low-Grade Squamous Intraepithelial Lesions (ASCUS-LSIL) Triage Study, we evaluated factors influencing the sensitivity of the enrollment colposcopic procedure. We used contingency table analysis to examine confounding variables and χ 2 tests to ascertain statistical significance. RESULTS: Overall, 69.9% of women with a cumulative diagnosis of CIN 3+ had a “ true-positive” enrollment colposcopically guided biopsy result of CIN 2 or worse (CIN 2+ ), the threshold that would trigger excisional therapy. The sensitivity of the procedure did not vary significantly by type of colposcopist. However, the sensitivity was significantly greater when the colposcopists took two or more biopsies instead of one (P < .01), a pattern observed across all types of colposcopists. Independent of the severity of the colposcopic impression, the frequency with which colposcopists took two or more biopsies instead of one varied (in descending order) from nurse practitioners to general gynecologists to gynecologic oncology fellows to gynecologic oncologists (P < .01). CONCLUSION: Colposcopy with guided biopsy or biopsies detects approximately two thirds of CIN 3+ . Although the sensitivity of the procedure does not differ significantly by type of medical training, it is greater when two or more biopsies are taken.展开更多
Sexual lifestyles are closely related to overall human health and well-being.Few studies have focused on sexual lifestyles among older adults living with HIV(OALHIV),especially in low-and middle-income countries.This ...Sexual lifestyles are closely related to overall human health and well-being.Few studies have focused on sexual lifestyles among older adults living with HIV(OALHIV),especially in low-and middle-income countries.This study is a part of the sexual well-being among older adults in China(SWELL)study,which is a multicenter cross-sectional study focused on sexual health among older adults aged 50 years and older.Participants were 680 OALHIV(≥50 years old)from the SWELL study conducted from June 2020 to December 2022.Data were collected through one-on-one interviews.We used logistic regression to assess the correlates of sexual activity and sexual satis-faction.Among all participants,37.1%were sexually active.Being older,male,in a steady relationship and employed were associated with being sexually active.The prevalence of sexual satisfaction was 69.8%among sexually active OALHIV.Being homosexual and reporting a better general health status were associated with sexual satisfaction.The OALHIV who have depressive symptoms were less likely to report sexual satisfaction.To support holistic healthy aging among OALHIV,it is important for healthcare providers to be educated about the importance of enquiring about sexual activity,satisfaction and problems and addressing concerns while conveying sex-positive attitudes during clinical reviews,as these are still critical aspects of their health and well-being.展开更多
Burn injuries and their treatments result in severe pain.Unlike traumatic injuries that are characterized by a discrete episode of pain followed by recovery,burn-injured patients endure pain for a prolonged period tha...Burn injuries and their treatments result in severe pain.Unlike traumatic injuries that are characterized by a discrete episode of pain followed by recovery,burn-injured patients endure pain for a prolonged period that lasts through wound closure(e.g.background pain,procedural pain,breakthrough pain,neuropathic pain and itch).Regional anesthesia,including peripheral nerve blocks and neuraxial/epidural anesthesia,offers significant benefits to a multimodal approach in pain treatment.A‘regional-first’approach to pain management can be incorporated into the workflow of burn centers through engaging regional anesthesiologists and pain medicine practitioners in the care of burn patients.A detailed understanding of peripheral nerve anatomy frames the burn clinician’s perspective when considering a peripheral nerve block/catheter.The infra/supraclavicular nerve block provides excellent coverage for the upper extremity,while the trunk can be covered with a variety of blocks including erector spinae plane and quadratus lumborum plane blocks.The lower extremity is targeted with fascia iliaca plane and sciatic nerve blocks for both donor and recipient sites.Burn centers that adopt regional anesthesia should be aware of potential complications and contraindications to prevent adverse events,including management of local anesthetic toxicity and epidural infections.Management of anticoagulation around regional anesthesia placement is crucial to prevent hematoma and nerve damage.Ultimately,regional anesthesia can facilitate a better patient experience and allow for early therapy and mobility goals that are hallmarks of burn care and rehabilitation.展开更多
A salient feature of neurons is their intrinsic ability to grow and extend neurites, even in the absence of external cues. Compared to the later stages of neuronal development, such as neuronal polarization and dendri...A salient feature of neurons is their intrinsic ability to grow and extend neurites, even in the absence of external cues. Compared to the later stages of neuronal development, such as neuronal polarization and dendrite morphogenesis, the early steps of neuritogenesis remain relatively unexplored. Here we showed that redistribution of cortical actin into large aggregates preceded neuritoge- nesis and determined the site of neurite initiation. Enhancing actin polymerization by jasplakinolide treat- ment effectively blocked actin redistribution and neurite initiation, while treatment with the actin depolymerizing agents latrunculin A or cytochalasin D accelerated neurite formation. Together, these results demonstrate a critical role of actin dynamics and reorganization in neurite initi- ation. Further experiments showed that microtubule dynamics and protein synthesis are not required for neurite initiation, but are required for later neurite stabilization. The redistribution of actin during early neuronal develop- ment was also observed in the cerebral cortex and hip- pocampus in vivo.展开更多
基金supported by the National Nature Science Foundation of China(Grant Number:61962010).
文摘Deepfake-generated fake faces,commonly utilized in identity-related activities such as political propaganda,celebrity impersonations,evidence forgery,and familiar fraud,pose new societal threats.Although current deepfake generators strive for high realism in visual effects,they do not replicate biometric signals indicative of cardiac activity.Addressing this gap,many researchers have developed detection methods focusing on biometric characteristics.These methods utilize classification networks to analyze both temporal and spectral domain features of the remote photoplethysmography(rPPG)signal,resulting in high detection accuracy.However,in the spectral analysis,existing approaches often only consider the power spectral density and neglect the amplitude spectrum—both crucial for assessing cardiac activity.We introduce a novel method that extracts rPPG signals from multiple regions of interest through remote photoplethysmography and processes them using Fast Fourier Transform(FFT).The resultant time-frequency domain signal samples are organized into matrices to create Matrix Visualization Heatmaps(MVHM),which are then utilized to train an image classification network.Additionally,we explored various combinations of time-frequency domain representations of rPPG signals and the impact of attention mechanisms.Our experimental results show that our algorithm achieves a remarkable detection accuracy of 99.22%in identifying fake videos,significantly outperforming mainstream algorithms and demonstrating the effectiveness of Fourier Transform and attention mechanisms in detecting fake faces.
基金National Natural Science Foundation of China under Grant Nos.61803152,31920103016,and 11871475Doctoral Start-Up Foundation of Hunan Normal University under Grant No.0531120-3827Hunan Provincial Education Department under Grant No.HNKCSZ-2020-0813.
文摘This study aims to improve control schemes for COVID-19 by a numerical model with estimation of parameters.We established a multi-level and multi-objective nonlinear SEIDR model to simulate the virus transmission.The early spread in Japan was adopted as a case study.The first 96 days since the infection were divided into five stages with parameters estimated.Then,we analyzed the trend of the parameter value,age structure ratio,and the defined PCR test index(standardization of the scale of PCR tests).It was discovered that the self-healing rate and confirmed rate were linear with the age structure ratio and the PCR test index using the stepwise regression method.The transmission rates were related to the age structure ratio,PCR test index,and isolation efficiency.Both isolation measures and PCR test medical screening can effectively reduce the number of infected cases based on the simulation results.However,the strategy of increasing PCR test medical screening would encountered a bottleneck effect on the virus control when the index reached 0.3.The effectiveness of the policy would decrease and the basic reproduction number reached the extreme value at 0.6.This study gave a feasible combination for isolation and PCR test by simulation.The isolation intensity could be adjusted to compensate the insufficiency of PCR test to control the pandemic.
文摘OBJECTIVE: To examine the influence that type of medical training and number of biopsies have on sensitivity of colposcopically guided biopsies. METHODS: Among 408 women with an adequate enrollment colposcopy and a diagnosis of cervical intraepithelial neoplasia (CIN) 3 or cancer (CIN 3+ ) over 2 years in the Atypical Squamous Cells of Undetermined Significance/Low-Grade Squamous Intraepithelial Lesions (ASCUS-LSIL) Triage Study, we evaluated factors influencing the sensitivity of the enrollment colposcopic procedure. We used contingency table analysis to examine confounding variables and χ 2 tests to ascertain statistical significance. RESULTS: Overall, 69.9% of women with a cumulative diagnosis of CIN 3+ had a “ true-positive” enrollment colposcopically guided biopsy result of CIN 2 or worse (CIN 2+ ), the threshold that would trigger excisional therapy. The sensitivity of the procedure did not vary significantly by type of colposcopist. However, the sensitivity was significantly greater when the colposcopists took two or more biopsies instead of one (P < .01), a pattern observed across all types of colposcopists. Independent of the severity of the colposcopic impression, the frequency with which colposcopists took two or more biopsies instead of one varied (in descending order) from nurse practitioners to general gynecologists to gynecologic oncology fellows to gynecologic oncologists (P < .01). CONCLUSION: Colposcopy with guided biopsy or biopsies detects approximately two thirds of CIN 3+ . Although the sensitivity of the procedure does not differ significantly by type of medical training, it is greater when two or more biopsies are taken.
基金supported by the Natural Science Foundation of China International/Regional Research Collaboration Project(72061137001)Natural Science Foundation of China Excellent Young Scientists Fund(82022064)+2 种基金Chongqing Talents Program for Innovative and Entrepreneurial Pioneers(cstc2021ycjh-bgzxm0097)Chongqing Natural Science Foundation Project(cstc2021jcyj-msxmX1171)Chinese State Key Laboratory of Infectious Disease Prevention and Control(2021SKLID303).
文摘Sexual lifestyles are closely related to overall human health and well-being.Few studies have focused on sexual lifestyles among older adults living with HIV(OALHIV),especially in low-and middle-income countries.This study is a part of the sexual well-being among older adults in China(SWELL)study,which is a multicenter cross-sectional study focused on sexual health among older adults aged 50 years and older.Participants were 680 OALHIV(≥50 years old)from the SWELL study conducted from June 2020 to December 2022.Data were collected through one-on-one interviews.We used logistic regression to assess the correlates of sexual activity and sexual satis-faction.Among all participants,37.1%were sexually active.Being older,male,in a steady relationship and employed were associated with being sexually active.The prevalence of sexual satisfaction was 69.8%among sexually active OALHIV.Being homosexual and reporting a better general health status were associated with sexual satisfaction.The OALHIV who have depressive symptoms were less likely to report sexual satisfaction.To support holistic healthy aging among OALHIV,it is important for healthcare providers to be educated about the importance of enquiring about sexual activity,satisfaction and problems and addressing concerns while conveying sex-positive attitudes during clinical reviews,as these are still critical aspects of their health and well-being.
文摘Burn injuries and their treatments result in severe pain.Unlike traumatic injuries that are characterized by a discrete episode of pain followed by recovery,burn-injured patients endure pain for a prolonged period that lasts through wound closure(e.g.background pain,procedural pain,breakthrough pain,neuropathic pain and itch).Regional anesthesia,including peripheral nerve blocks and neuraxial/epidural anesthesia,offers significant benefits to a multimodal approach in pain treatment.A‘regional-first’approach to pain management can be incorporated into the workflow of burn centers through engaging regional anesthesiologists and pain medicine practitioners in the care of burn patients.A detailed understanding of peripheral nerve anatomy frames the burn clinician’s perspective when considering a peripheral nerve block/catheter.The infra/supraclavicular nerve block provides excellent coverage for the upper extremity,while the trunk can be covered with a variety of blocks including erector spinae plane and quadratus lumborum plane blocks.The lower extremity is targeted with fascia iliaca plane and sciatic nerve blocks for both donor and recipient sites.Burn centers that adopt regional anesthesia should be aware of potential complications and contraindications to prevent adverse events,including management of local anesthetic toxicity and epidural infections.Management of anticoagulation around regional anesthesia placement is crucial to prevent hematoma and nerve damage.Ultimately,regional anesthesia can facilitate a better patient experience and allow for early therapy and mobility goals that are hallmarks of burn care and rehabilitation.
基金the ION Optical Imaging Core Facility for technical supportthe IOBS-Nikon Biological Imaging Center for use of the N-SIM microscopesupported by grants from the National Natural Science Foundation of China(31125015 and31321091)
文摘A salient feature of neurons is their intrinsic ability to grow and extend neurites, even in the absence of external cues. Compared to the later stages of neuronal development, such as neuronal polarization and dendrite morphogenesis, the early steps of neuritogenesis remain relatively unexplored. Here we showed that redistribution of cortical actin into large aggregates preceded neuritoge- nesis and determined the site of neurite initiation. Enhancing actin polymerization by jasplakinolide treat- ment effectively blocked actin redistribution and neurite initiation, while treatment with the actin depolymerizing agents latrunculin A or cytochalasin D accelerated neurite formation. Together, these results demonstrate a critical role of actin dynamics and reorganization in neurite initi- ation. Further experiments showed that microtubule dynamics and protein synthesis are not required for neurite initiation, but are required for later neurite stabilization. The redistribution of actin during early neuronal develop- ment was also observed in the cerebral cortex and hip- pocampus in vivo.
