Evaluation of direct intramural injection to the bladder wall as a method for developing orthotopic tumor models

Background:Bladder cancer poses a great burden on society and its high rate of recurrence and treatment failure necessitates use of appropriate animal models to study its pathogenesis and test novel treatments.Orthotopic models are superior to other types since they provide a normal microenvironment.Four methods are described for developing bladder cancer models inside the animal’s bladder.Direct intramural injection is one of these methods and is widely used.However,its efficacy in model development has not yet been studied.We aimed to evaluate the efficacy and success rate of the direct intramural injection method of developing an orthotopic model for the study of bladder cancer.Method:Tumor cell lines were prepared in four microtubes.Aliquots of 200×10^(3) cells were injected through a 27 gauge needle into the ventral wall of the bladders of 4male and 4 female BALB/c mice following a midline 1 cm laparotomy incision.In addition,1 million cells from each microtube were injected into the flanks of control mice.To prevent infection and alleviate pain,5 mg/kg enrofloxacin and 2.5 mg/kg flunixin meglumine,respectively,were injected subcutaneously.Results:Tumors formed in all mice,resulting in 100% take rate and zero post-operation mortality.Surgery time was≤15 min per mouse.In two mice,tumors were found in the peritoneal space as well.Conclusion:Direct intramural injection is a rapid,reliable,and reproducible method for developing orthotopic models of bladder cancer.It can be done on both male and female mice and only requires readily available surgical tools.However,needle track can result in cell spillage and peritoneal tumors.

The Role and Function of Ras-association domain family in Cancer:A Review

Ras gene mutation has been observed in more than 30%of cancers,and 90%of pancreatic,lung and colon cancers.Ras proteins(K-Ras,H-Ras,N-Ras)act as molecular switches which are activated by binding to GTP.They play a role in the cascade of cell process control(proliferation and cell division).In the inactive state,transforming GTP to GDP leads to the activation of GTpase in Ras gene.However,the mutation in Ras leads to the loss of internal GTPase activity and permanent activation of the protein.The activated Ras can promote the cell death or stop cell growth,which are facilitated by Ras-association domain family.Various studies have been conducted to determine the importance of losing RASSF proteins in Rasinduced tumors.This paper examines the role of Ras and RASSF proteins.In general,RASSF proteins can be used as a suitable means for targeting a large group of Ras-induced tumors.

Human microbiome and prostate cancer development:current insights into the prevention and treatment

The huge communities of microorganisms that symbiotically colonize humans are recognized as significant players in health and disease.The human microbiome may influence prostate cancer development.To date,several studies have focused on the effect of prostate infections as well as the composition of the human microbiome in relation to prostate cancer risk.Current studies suggest that the microbiota of men with prostate cancer significantly differs from that of healthy men,demonstrating that certain bacteria could be associated with cancer development as well as altered responses to treatment.In healthy individuals,the microbiome plays a crucial role in the maintenance of homeostasis of body metabolism.Dysbiosis may contribute to the emergence of health problems,including malignancy through affecting systemic immune responses and creating systemic inflammation,and changing serum hormone levels.In this review,we discuss recent data about how the microbes colonizing different parts of the human body including urinary tract,gastrointestinal tract,oral cavity,and skin might affect the risk of developing prostate cancer.Furthermore,we discuss strategies to target the microbiome for risk assessment,prevention,and treatment of prostate cancer.