DNA studies of endangered or extinct species often rely on ancient or degraded remains.The majority of ancient DNA(aDNA)extraction protocols focus on skeletal elements,with skin and hair samples rarely explored.Simila...DNA studies of endangered or extinct species often rely on ancient or degraded remains.The majority of ancient DNA(aDNA)extraction protocols focus on skeletal elements,with skin and hair samples rarely explored.Similar to that found in bones and teeth,DNA extracted from historical or ancient skin and fur samples is also extremely fragmented with low endogenous content due to natural degradation processes.Thus,the development of effective DNA extraction methods is required for these materials.Here,we compared the performance of two DNA extraction protocols(commercial and custom laboratory aDNA methods)on hair and skin samples from decades-old museum specimens to Iron Age archaeological material.We found that apart from the impact sample-specific taphonomic and handling history has on the quantity and quality of DNA preservation,skin yielded more endogenous DNA than hair of the samples and protocols tested.While both methods recovered DNA from ancient soft tissue,the laboratory method performed better overall in terms of DNA yield and quality,which was primarily due to the poorer performance of the commercial binding buffer in recovering aDNA.展开更多
In line with the"ecosystem approach"of the Convention on Biological Diversity(CBD)(Secretariat of the Convention on Biological Diversity,2004)toward recognizing the service value in maintaining the essential...In line with the"ecosystem approach"of the Convention on Biological Diversity(CBD)(Secretariat of the Convention on Biological Diversity,2004)toward recognizing the service value in maintaining the essential balance of biodiversity,there has been a conceptual shift toward conservation frameworks that integrate a wider range of dedicated conservationists(Pearson,2016).In this paper,we explore the saker falcon Falco cherrug(hereafter saker)to illustrate how intrinsic service and cultural values within this conceptual paradigm enhance its conservation.展开更多
基金This work was supported by the Chinese Academy of Sciences(CAS,XDB26000000)National Natural Science Foundation of China(41925009,41630102,41672021)+1 种基金CAS(XDA1905010,QYZDB-SSW-DQC003),“Research on the Roots of Chinese Civilization”of Zhengzhou University(XKZDJC202006)Tencent Foundation through the XPLORER PRIZE,and Howard Hughes Medical Institute(55008731)。
文摘DNA studies of endangered or extinct species often rely on ancient or degraded remains.The majority of ancient DNA(aDNA)extraction protocols focus on skeletal elements,with skin and hair samples rarely explored.Similar to that found in bones and teeth,DNA extracted from historical or ancient skin and fur samples is also extremely fragmented with low endogenous content due to natural degradation processes.Thus,the development of effective DNA extraction methods is required for these materials.Here,we compared the performance of two DNA extraction protocols(commercial and custom laboratory aDNA methods)on hair and skin samples from decades-old museum specimens to Iron Age archaeological material.We found that apart from the impact sample-specific taphonomic and handling history has on the quantity and quality of DNA preservation,skin yielded more endogenous DNA than hair of the samples and protocols tested.While both methods recovered DNA from ancient soft tissue,the laboratory method performed better overall in terms of DNA yield and quality,which was primarily due to the poorer performance of the commercial binding buffer in recovering aDNA.
基金the National Key Program of Research and Development,Ministry of Science and Technology(2016YFC0503200)the National Natural Science Foundation of China(31930013,31900340)+1 种基金Biodiversity Survey,Monitoring and Assessment Project(2019-2023)of Ministry of Ecology and Environment,Chinathe Second Tibetan Plateau Scientific Expedition and Research Program(STEP)(2019QZKK0501)。
文摘In line with the"ecosystem approach"of the Convention on Biological Diversity(CBD)(Secretariat of the Convention on Biological Diversity,2004)toward recognizing the service value in maintaining the essential balance of biodiversity,there has been a conceptual shift toward conservation frameworks that integrate a wider range of dedicated conservationists(Pearson,2016).In this paper,we explore the saker falcon Falco cherrug(hereafter saker)to illustrate how intrinsic service and cultural values within this conceptual paradigm enhance its conservation.
