Taxus chinensis var.mairei is an endangered and economically important tree species in China.Four polymorphic microsatellite markers were developed and characterized using the fast isolation by amplified fragment leng...Taxus chinensis var.mairei is an endangered and economically important tree species in China.Four polymorphic microsatellite markers were developed and characterized using the fast isolation by amplified fragment length polymorphism(AFLP)of sequences containing repeats(FIASCO)protocol.Polymorphism of each locus was quantified for five natural populations from Hubei Province,China.The number of alleles per locus varied from 6 to 24.The ranges of observed and expected heterozygosity were 0.493–1.000 and 0.440–0.845,respectively.These are the first microsatellites reported for the family of Taxaceae and will be helpful for the ongoing population and conservation genetics research of Taxus chinensis var.mairei.展开更多
Cymbidium goeringii is a diploid and non-rewarding,bumblebee-pollinated species,which is distribut-ed in China,Japan and Korea Peninsula.This species is now highly endangered due to the mass collection and forest clea...Cymbidium goeringii is a diploid and non-rewarding,bumblebee-pollinated species,which is distribut-ed in China,Japan and Korea Peninsula.This species is now highly endangered due to the mass collection and forest clearance in China.In the present study,we investigated the distribution of genetic variation within and between eleven populations of Cymbidium goeringii in central China by using Inter-simple sequence repeats(ISSR)markers.Eleven primers produced a total of 127 clear and reproducible bands of which 112 were polymorphic.High genetic diversity was detected in Cymbidium goeringii for both population level(P=63.1%;He=0.1945)and species level(P=88.2%;He=0.2628).A higher level of genetic differentiation was detected among populations(G_(ST)=0.2440,F_(ST)=0.2207)with Nei’s GST analysis and analysis of molecular variance(AMOVA),and no correlation was found between geograph-ical and genetic distance.Genetic drift rather than gene flow played an important role in forming the present population structure of Cymbidium goeringii.Limited gene flow among populations and gene drift increase the extinction risk of local populations.Some conservation concerns are therefore dis-cussed together with possible strategies for implementing in situ and ex situ conservation.展开更多
基金This project was supported by the National Natural Science Foundation of China(Grant No.30771763)the“100 Talent Project”of the Chinese Academy of Sciences(No.:0729281F02)the“Outstanding Young Scientists Project”of the Natural Science Foundation of Hubei Province,China(No.O631061H01).
文摘Taxus chinensis var.mairei is an endangered and economically important tree species in China.Four polymorphic microsatellite markers were developed and characterized using the fast isolation by amplified fragment length polymorphism(AFLP)of sequences containing repeats(FIASCO)protocol.Polymorphism of each locus was quantified for five natural populations from Hubei Province,China.The number of alleles per locus varied from 6 to 24.The ranges of observed and expected heterozygosity were 0.493–1.000 and 0.440–0.845,respectively.These are the first microsatellites reported for the family of Taxaceae and will be helpful for the ongoing population and conservation genetics research of Taxus chinensis var.mairei.
基金We are grateful to Drs Li Zuozhou,Chen Lei and Liu Yifei for their assistance and advice and Mrs Zhang Chunxia provided assistance in the field observation and collecting samples.The work was supported by the Natural Scientific Foundation of Hubei Province,China(2004ABA119)for Yang Bo.
文摘Cymbidium goeringii is a diploid and non-rewarding,bumblebee-pollinated species,which is distribut-ed in China,Japan and Korea Peninsula.This species is now highly endangered due to the mass collection and forest clearance in China.In the present study,we investigated the distribution of genetic variation within and between eleven populations of Cymbidium goeringii in central China by using Inter-simple sequence repeats(ISSR)markers.Eleven primers produced a total of 127 clear and reproducible bands of which 112 were polymorphic.High genetic diversity was detected in Cymbidium goeringii for both population level(P=63.1%;He=0.1945)and species level(P=88.2%;He=0.2628).A higher level of genetic differentiation was detected among populations(G_(ST)=0.2440,F_(ST)=0.2207)with Nei’s GST analysis and analysis of molecular variance(AMOVA),and no correlation was found between geograph-ical and genetic distance.Genetic drift rather than gene flow played an important role in forming the present population structure of Cymbidium goeringii.Limited gene flow among populations and gene drift increase the extinction risk of local populations.Some conservation concerns are therefore dis-cussed together with possible strategies for implementing in situ and ex situ conservation.
