Stable and single-dispersed hydroxyapatite （HAP） nanoparticles were synthesized with ultrasonic-assisted method. HAP nanoparticles were characterized by dynamic light scattering, XRD （X-ray diffraction） and TEM （...Stable and single-dispersed hydroxyapatite （HAP） nanoparticles were synthesized with ultrasonic-assisted method. HAP nanoparticles were characterized by dynamic light scattering, XRD （X-ray diffraction） and TEM （Transmission Electron Microscopy）. The effect of HAP nanoparticles on the K562 human myelogenous leukemia cell line was investigated by MTT assay and cell count test, and the mechanism was studied through the changes of cell cycle and ultrastructure. The results showed that HAP nanoparticles inhibited the proliferation of K562 cells dramatically in vitro. HAP nanoparticles entered the cytoplasm of K562 cells and the cells were arrested at G/M phase, thus, the cells died directly.展开更多
Through the Agility of Agile Transportation key concept and the C^3I^2 mode of information fusion, the realization mechanism of Agile Transportation is to be discussed for analyzing influence of which the idea of mult...Through the Agility of Agile Transportation key concept and the C^3I^2 mode of information fusion, the realization mechanism of Agile Transportation is to be discussed for analyzing influence of which the idea of multilateral virtual alliance works on Agility of transportation enterprise. By research on the agile transportation based on network information fusion, middle layers axe reduced, and the pyramid management mode is transformed to fiat management mode. The modern logistic technology is used to enhance the ability of multilateral virtual alliance --cooperation ability, flexible dispatching ability of logistic node, speediness transportation ability, response ability of transportation market change , to become accessible , integrated , efficient, offers flexibility of choices, convenient transportation system.展开更多
基金the Science and Technique Foundation of Hubei Province(No.2004AA303B03)
文摘Stable and single-dispersed hydroxyapatite （HAP） nanoparticles were synthesized with ultrasonic-assisted method. HAP nanoparticles were characterized by dynamic light scattering, XRD （X-ray diffraction） and TEM （Transmission Electron Microscopy）. The effect of HAP nanoparticles on the K562 human myelogenous leukemia cell line was investigated by MTT assay and cell count test, and the mechanism was studied through the changes of cell cycle and ultrastructure. The results showed that HAP nanoparticles inhibited the proliferation of K562 cells dramatically in vitro. HAP nanoparticles entered the cytoplasm of K562 cells and the cells were arrested at G/M phase, thus, the cells died directly.
文摘Through the Agility of Agile Transportation key concept and the C^3I^2 mode of information fusion, the realization mechanism of Agile Transportation is to be discussed for analyzing influence of which the idea of multilateral virtual alliance works on Agility of transportation enterprise. By research on the agile transportation based on network information fusion, middle layers axe reduced, and the pyramid management mode is transformed to fiat management mode. The modern logistic technology is used to enhance the ability of multilateral virtual alliance --cooperation ability, flexible dispatching ability of logistic node, speediness transportation ability, response ability of transportation market change , to become accessible , integrated , efficient, offers flexibility of choices, convenient transportation system.
