Influenza virus is a continuous and severe global threat to mankind. The continuously re-emerging disease gives rise to thousands of deaths and enormous economic losses each year, which emphasizes the urgency and nece...Influenza virus is a continuous and severe global threat to mankind. The continuously re-emerging disease gives rise to thousands of deaths and enormous economic losses each year, which emphasizes the urgency and necessity to develop high-quality influenza vaccines in a safer, more efficient and economic way. The influenza subunit and VLP vaccines, taking the advantage of recombinant DNA technologies and expression system platforms, can be produced in such an ideal way. This review summarized the recent advancements in the research and development of influenza subunit and VLP vaccines based on the recombinant expression of hemagglutinin antigen (HA), neuraminidase antigen (NA), Matrix 2 protein (M2) and nucleocapsid protein (NP). It would help to get insight into the current stage of influenza vaccines, and suggest the future design and development of novel influenza vaccines.展开更多
Background The YIGSR is a pentapeptide, from the laminin-1 of the β1 chain, which can mediate cell adhesion and bind the 67 kD laminin receptor. The purpose is to evaluate the usefulness of 99m Tc-YIGSR, a novel ...Background The YIGSR is a pentapeptide, from the laminin-1 of the β1 chain, which can mediate cell adhesion and bind the 67 kD laminin receptor. The purpose is to evaluate the usefulness of 99m Tc-YIGSR, a novel tumour radiotracer, in the receptor imaging of Ehrlich ascites tumour. Methods Using S-Acetly-NH_3-MAG_3 as chelate, YIGSR, a pentapeptide from laminin, was tagged with 99m Tc. 99m Tc-YIGSR was detected in the tumour group bearing Ehrlich ascites tumour and blocked group. Tumour, normal, inflammatory and blocked groups were imaged. Results Through reverse phase Sep-Pak C_ 18 chromatogram, it was revealed that YIGSR could conjugate with S-Acetly -NH_3-MAG_3, and be radiolabelled at room temperature and neutral pH with a radiolabelling yield of 62%, and of 4% without chelate. 99m Tc-YIGSR was rapidly cleared from kidney, then liver. The imaging findings showed tumour tissue accumulated initial radioactivity at fifteen minutes after injection in the tumour group, and the uptake increased to peak at three hours with a tumour/muscle ratio (T/M) of 11.36, then cleared slowly to a T/M of 7.50 at eight hours. The tumour uptake of radiotracer in blocked group was significantly lower with T/M of 4.61 at three hours and 0.89 at eight hours. The T/M was only 3.72 at three hours and 1.29 at eight hours after injection in inflammatory group. Compared with inflammatory group and control obstructive group, the ratio of T/M in tumour group was significantly different ( P <0.001). Conclusions Using S-Acetly-NH_3-MAG_3, we radiolabelled YIGSR with 99m Tc. 99m Tc-YIGSR possesses many merits of tumour imaging: rapid visualization, high sensitivity and specificity, and satisfactory target/nontarget ratio. Our data suggest 99m Tc-YIGSR is a promising tumour radiotracer.展开更多
A large quantity of SARS-CoV virus was pro-liferated in Vero cells, inactivated with b-propiolactone, then purified by Sepharose 4FF column chromatography to pre-pare inactivated vaccine. The vaccine was identified by...A large quantity of SARS-CoV virus was pro-liferated in Vero cells, inactivated with b-propiolactone, then purified by Sepharose 4FF column chromatography to pre-pare inactivated vaccine. The vaccine was identified by Western blot, mass spectrographic analysis, ELISA and elec-tron microscopy. The vaccine with or without aluminum hydroxide adjuvant was inoculated into female BALB/c mice at different dosages. The result showed that the antibodies to SARS-CoV were induced in the mice. The antibody levels induced by the vaccine with aluminum hydroxide were higher than those without aluminum hydroxide.展开更多
基金The Knowledge Innovation Program of the Chinese Academy of Sciences (Grant No. KSCX2-EW-G-8)
文摘Influenza virus is a continuous and severe global threat to mankind. The continuously re-emerging disease gives rise to thousands of deaths and enormous economic losses each year, which emphasizes the urgency and necessity to develop high-quality influenza vaccines in a safer, more efficient and economic way. The influenza subunit and VLP vaccines, taking the advantage of recombinant DNA technologies and expression system platforms, can be produced in such an ideal way. This review summarized the recent advancements in the research and development of influenza subunit and VLP vaccines based on the recombinant expression of hemagglutinin antigen (HA), neuraminidase antigen (NA), Matrix 2 protein (M2) and nucleocapsid protein (NP). It would help to get insight into the current stage of influenza vaccines, and suggest the future design and development of novel influenza vaccines.
文摘Background The YIGSR is a pentapeptide, from the laminin-1 of the β1 chain, which can mediate cell adhesion and bind the 67 kD laminin receptor. The purpose is to evaluate the usefulness of 99m Tc-YIGSR, a novel tumour radiotracer, in the receptor imaging of Ehrlich ascites tumour. Methods Using S-Acetly-NH_3-MAG_3 as chelate, YIGSR, a pentapeptide from laminin, was tagged with 99m Tc. 99m Tc-YIGSR was detected in the tumour group bearing Ehrlich ascites tumour and blocked group. Tumour, normal, inflammatory and blocked groups were imaged. Results Through reverse phase Sep-Pak C_ 18 chromatogram, it was revealed that YIGSR could conjugate with S-Acetly -NH_3-MAG_3, and be radiolabelled at room temperature and neutral pH with a radiolabelling yield of 62%, and of 4% without chelate. 99m Tc-YIGSR was rapidly cleared from kidney, then liver. The imaging findings showed tumour tissue accumulated initial radioactivity at fifteen minutes after injection in the tumour group, and the uptake increased to peak at three hours with a tumour/muscle ratio (T/M) of 11.36, then cleared slowly to a T/M of 7.50 at eight hours. The tumour uptake of radiotracer in blocked group was significantly lower with T/M of 4.61 at three hours and 0.89 at eight hours. The T/M was only 3.72 at three hours and 1.29 at eight hours after injection in inflammatory group. Compared with inflammatory group and control obstructive group, the ratio of T/M in tumour group was significantly different ( P <0.001). Conclusions Using S-Acetly-NH_3-MAG_3, we radiolabelled YIGSR with 99m Tc. 99m Tc-YIGSR possesses many merits of tumour imaging: rapid visualization, high sensitivity and specificity, and satisfactory target/nontarget ratio. Our data suggest 99m Tc-YIGSR is a promising tumour radiotracer.
文摘A large quantity of SARS-CoV virus was pro-liferated in Vero cells, inactivated with b-propiolactone, then purified by Sepharose 4FF column chromatography to pre-pare inactivated vaccine. The vaccine was identified by Western blot, mass spectrographic analysis, ELISA and elec-tron microscopy. The vaccine with or without aluminum hydroxide adjuvant was inoculated into female BALB/c mice at different dosages. The result showed that the antibodies to SARS-CoV were induced in the mice. The antibody levels induced by the vaccine with aluminum hydroxide were higher than those without aluminum hydroxide.
